Ochratoxin A Detection on Antibody- Immobilized on BSA-Functionalized Gold Electrodes

Badea, Mihaela; Floroian, Laura; Restani, Patrizia; Cobzac, Simona Codruța Aurora; Moga, Marius

doi:10.1371/journal.pone.0160021

Cited by 31 publications

(11 citation statements)

References 36 publications

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“…The antibody solution (100 ng/mL) was then pipetted over the surface (1 h room temperature, humid atmosphere). For the deactivation of the remaining succinimide groups and in order to block unreacted active sites 1 M ethanolamine–HCl was added for 15 min in the dark [ 30 ]. The modified electrodes were stored dry overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

Mavrikou

Moschopoulou

Zafeirakis³

et al. 2018

Sensors

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Prostate-specific antigen (PSA) is the established routine screening tool for the detection of early-stage prostate cancer. Given the laboratory-centric nature of the process, the development of a portable, ultra rapid high-throughput system for PSA screening is highly desirable. In this study, an advancedpoint-of-care system for PSA detection in human serum was developed based on a cellular biosensor where the cell membrane was modified by electroinserting a specific antibody against PSA. Thirty nine human serum samples were used for validation of this biosensory system for PSA detection. Samples were analyzed in parallel with a standard immunoradiometric assay (IRMA) and an established electrochemical immunoassay was used for comparison purposes. They were classified in three different PSA concentration ranges (0, <4 and ≥4 ng/mL). Cells membrane-engineered with 0.25 μg/mL anti-PSA antibody demonstrated a statistically lower response against the upper (≥4 ng/mL) PSA concentration range. In addition, the cell-based biosensor performed better than the immunosensor in terms of sensitivity and resolution against positive samples containing <4 ng/mL PSA. In spite of its preliminary, proof-of-concept stage of development, the cell-based biosensor could be used as aninitiative for the development of a fast, low-cost, and high-throughput POC screening system for PSA.

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Section: Methodsmentioning

confidence: 99%

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

Mavrikou

Moschopoulou

Zafeirakis³

et al. 2018

Sensors

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“…The analytical performance of the electrochemical aptasensor was compared with published studies on the electrochemical detection of OTA, as shown in Table S4. Significantly, the current approach provides a lower LOD and wider working range compared to other silver metallization assays. − The LOD of our system is marginally higher than the platforms presented in refs , ; however, it is important to note that the linear range in the current system is much wider and the method far simpler to implement due to the use of disposable SPGEs.…”

Section: Resultsmentioning

confidence: 84%

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

et al. 2019

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Ochratoxin A (OTA)a mycotoxin produced by Aspergillus and Penicillium fungiis a carcinogen and common trace contaminant in agricultural and processed food products. As consumption is detrimental to human and animal health, regular product monitoring is vital, and highly sensitive and portable OTA sensors are necessary in many circumstances. Herein, we report an ultrasensitive, electroanalytical aptasensor for precise determination of OTA at trace levels. The sensor leverages a DNA aptamer to capture OTA and silver metallization as a signal enhancer. Exonuclease I is used to digest unbound aptamers, engendering excellent background signal suppression and sensitivity enhancements. Efficient optimization of assay conditions is achieved using central composite design (CCD), allowing rapid evaluation of both the electrode and square wave voltammetry parameter space. The sensor exhibits excellent analytical performance, with a concentration limit of detection of 0.7 pg mL −1 , a limit of quantitation of 2.48 pg mL −1 , and a linear dynamic range (R 2 = 0.968) of over 6 orders of magnitude (between 1 pg mL −1 and 0.1 μg mL −1 ). Direct comparison with ultraperformance liquid chromatography (UPLC) indicates excellent analytical performance for standard solutions (R 2 = 0.995) and spiked beer samples (R 2 = 0.993), with almost quantitative recovery and less than 5% relative standard deviation (RSD).

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“…From the results showed above is deducted that by the two types of electrochemical methods using screenprinted carbon electrodes (DPV and CV) and Jenco microelectrodes (for iodide detection), iodide can be easily determined qualitatively and quantitatively. Both methods are safe and easy to use, and can be thought of as a portable system with a wide range of applicability [34][35][36][37][38][39][40] (screen electrodes are already used to detect toxic and bioactive compounds in the life sciences), alongside other validated methods for the same purpose [41][42][43][44][45][46][47]. Redox microelectrodes correlate in a good manner the redox status of the solution with KI concentration.…”

Section: B2 Redox Microelectrodementioning

confidence: 99%

New Electrochemical Detection Strategies for Iodinated Compounds

Chelmea¹,

Modugno²,

Samota³

et al. 2019

Rev. Chim.

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Iodine is a micronutrient of high importance for the health and good development of individuals. It is contained in many foods, but it is also part of the chemical component of oceans and soil. Its deficiency is still a problem of humanity, which has a serious repercussion on our health. It is therefore important to have specific, fast and cost effective methods of iodine detection from different samples. This work aims to identify optimal parameters for potassium iodide (KI) detection from different media, to be further applied to real samples: plant extracts, water, biological fluids. Results showed a significant difference in electrochemical results, depending on the pH values of the mixture and also on the time which influences the compounds stability. Differential pulse voltammetry and cyclic voltammetry using carbon printed sensors, as well as microsensors for redox status or direct iodide detection are important analytical tools which have a wide range of applications in the food, medicine, toxicology and other domains.

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Ochratoxin A Detection on Antibody- Immobilized on BSA-Functionalized Gold Electrodes

Cited by 31 publications

References 36 publications

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

New Electrochemical Detection Strategies for Iodinated Compounds

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