2011
DOI: 10.1080/19440049.2010.508796
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Ochratoxin A in cocoa and chocolate sampled in Canada

Abstract: In order to determine the levels of ochratoxin A (OTA) in cocoa and cocoa products available in Canada, a previously published analytical method, with minor modifications to the extraction and immunoaffinity clean-up and inclusion of an evaporation step, was initially used (Method I). To improve the low method recoveries (46–61%), 40% methanol was then included in the aqueous sodium bicarbonate extraction solvent (pH 7.8) (Method II). Clean-up was on an Ochratest™ immunoaffinity column and OTA was determined b… Show more

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Cited by 26 publications
(19 citation statements)
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“…Data shows presence of ochratoxin A in 92e100% of the products evaluated, with average levels ranging from 0.17 to 2.41 mg/kg (Bonvehi, 2004;Brera et al, 2011;Burdaspal & Legarda, 2003;Engel, 2000;Gilmour & Lindblom, 2008;Miraglia & Brera, 2002). The incidence of ochratoxin A reported for chocolate bars is also high, 60e100% in dark chocolate (0.14e0.38 mg/kg) (Brera et al, 2011;Burdaspal & Legarda, 2003;Engel, 2000;Gilmour & Lindblom, 2008;Kumagai et al, 2008;Turcotte & Scott, 2011), 23e100% in milk chocolate (0.08e0.16 mg/kg) (Brera et al, 2011;Burdaspal & Legarda, 2003;Engel, 2000;Turcotte & Scott, 2011), and 88e100% of contamination in white chocolate (0.03 mg/kg) (Burdaspal & Legarda, 2003).…”
Section: Resultsmentioning
confidence: 94%
See 1 more Smart Citation
“…Data shows presence of ochratoxin A in 92e100% of the products evaluated, with average levels ranging from 0.17 to 2.41 mg/kg (Bonvehi, 2004;Brera et al, 2011;Burdaspal & Legarda, 2003;Engel, 2000;Gilmour & Lindblom, 2008;Miraglia & Brera, 2002). The incidence of ochratoxin A reported for chocolate bars is also high, 60e100% in dark chocolate (0.14e0.38 mg/kg) (Brera et al, 2011;Burdaspal & Legarda, 2003;Engel, 2000;Gilmour & Lindblom, 2008;Kumagai et al, 2008;Turcotte & Scott, 2011), 23e100% in milk chocolate (0.08e0.16 mg/kg) (Brera et al, 2011;Burdaspal & Legarda, 2003;Engel, 2000;Turcotte & Scott, 2011), and 88e100% of contamination in white chocolate (0.03 mg/kg) (Burdaspal & Legarda, 2003).…”
Section: Resultsmentioning
confidence: 94%
“…In the last decade, reports on the occurrence of ochratoxin A in cocoa and chocolate have increased (Bonvehi, 2004;Brera et al, 2011;Burdaspal & Legarda, 2003;Copetti et al, 2010;Engel, 2000;Gilmour & Lindblom, 2008;Kumagai et al, 2008;Turcotte & Scott, 2011), although data on the presence of aflatoxins in this product are very limited (Copetti et al, 2011;Kumagai et al, 2008;Raters & Matissek, 2000). The increasing restriction on the presence of both toxins in foods together with the availability of improved equipment and more sensitive analytical techniques allows a better control of public exposure to these contaminants.…”
Section: Introductionmentioning
confidence: 96%
“…(1.8 mL) by syringe pressure through a 0.45 mm Teflon 4-mm filter for HPLC analysis under the same conditions as for OTA (Turcotte and Scott 2011).…”
Section: Determination Of Ochratoxin a By Hplc 1499mentioning
confidence: 99%
“…They are solid at room-temperature (RT) but converts into an apolar sol at $38-39°C, depending on the source of extraction [23]. These fats are being extensively used in various food, cosmetics, and pharmaceutical industries due to their beneficial effects [24,25].…”
Section: Introductionmentioning
confidence: 99%