Ochrobactrum cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress

Burygin, Gennady L.; Kargapolova, K.Yu.; Kryuchkova, Yelena V.; Avdeeva, Elena; Gogoleva, Natalia; Ponomaryova, Тatiana S.; Tkachenko, Oksana V.

doi:10.1007/s11274-019-2633-x

Cited by 28 publications

(26 citation statements)

References 60 publications

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“…The gANI analysis also indicated that O. cytisi IPA7.2 can be clearly separated from O. anthropi genomes (gANI in the range 95.47-95.99) despite a very close phylogenetic relationship (Figure 2). Although the sequenced strain is not the type strain of the species, a multilocus analysis showed previously that IPA7.2 is phylogenetically closer to the O. cytisi type strain than to any O. anthropi strain (Burygin et al, 2019). These results support the status of O. cytisi as a separate species, a status which have been recently questioned based on 16S rRNA, MLSA and DDH analyses (Gazolla Volpiano et al, 2019).…”

Section: Delimitation Of Ochrobactrum Genomic Speciesmentioning

confidence: 55%

“…It should also be noted that all Brucella species form a monophyletic cluster inside Clade 1 with maximum support. The inclusion of Brucella among Ochrobactrum species was recurrently observed within previous studies, but the exact positioning have never been conclusively resolved (Zurdo-Pineiro et al, 2007;Huber et al, 2009;Burygin et al, 2019). Specifically, the species O. intermedium was named after its supposed closest relationship to Brucella spp.…”

Section: Phylogenomic Relationships Among Brucellaceaementioning

confidence: 94%

“…could be drawn because of the underlying lack of phylogenetic resolution. Limited multilocus sequence typing (MLST) or Internal Transcribed Spacer (ITS) region-based analyses were also performed but were hampered by a small set of genes, a small set of species, or poorly supported branching, and still resulted in conflicting results (Zurdo-Pineiro et al, 2007;Aujoulat et al, 2014;Burygin et al, 2019;Gazolla Volpiano et al, 2019;Krzyżanowska et al, 2019). More recently, whole genome phylogenetic studies started to emerge but generally focused on a limited number of isolates of few species and could not provide a comprehensive view of the phylogenetic relationships at the family level or even at the Ochrobactrum genus level (Gazolla Volpiano et al, 2019;Krzyżanowska et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Taxonomic Organization of the Family Brucellaceae Based on a Phylogenomic Approach

2020

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Deciphering the evolutionary history of pathogenic bacteria and their near neighbors may help to understand the genetic or ecological bases which led to their pathogenic behavior. The Brucellaceae family comprises zoonotic pathogenic species belonging to the genus Brucella as well as the environmental genus Ochrobactrum for which some species are considered as opportunistic pathogens. Here, we used a phylogenomic approach including a set of 145 Brucellaceae genomes representative of the family diversity and more than 40 genomes of the order Rhizobiales to infer the taxonomic relationships between the family's species. Our results clarified some unresolved phylogenetic ambiguities, conducting to the exclusion of Mycoplana spp. out of the family Brucellaceae and the positioning of all Brucella spp. as a single genomic species within the current Ochrobactrum species diversity. Additional analyses also revealed that Ochrobactrum spp. separate into two clades, one comprising mostly environmental species while the other one includes the species considered as pathogens (Brucella spp.) or opportunistic pathogens (mainly O. anthropi, O. intermedium, and O. pseudintermedium). Finally, we show that O. intermedium is undergoing a beginning of genome reduction suggestive of an ongoing ecological niche specialization, and that some lineages of O. intermedium and O. anthropi may shift toward an adaption to the human host.

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Section: Delimitation Of Ochrobactrum Genomic Speciesmentioning

confidence: 55%

Section: Phylogenomic Relationships Among Brucellaceaementioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Taxonomic Organization of the Family Brucellaceae Based on a Phylogenomic Approach

2020

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“…O. cytisi IPA7.2 promotes potato growth and is promising for association with plants. The strain is halotolerant: bacterial growth stops at 750 mM NaCl [ 25 ]. Bacteria were grown in liquid synthetic malate salt medium [ 26 ] at 35 °C (optimal growth temperature for both strains) in an ES-20/60 incubator shaker (Biosan, Latvia) at 150 rpm.…”

Section: Methodsmentioning

confidence: 99%

Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress

et al. 2020

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Water deficits inhibit plant growth and decrease crop productivity. Remedies are needed to counter this increasingly urgent problem in practical farming. One possible approach is to utilize rhizobacteria known to increase plant resistance to abiotic and other stresses. We therefore studied the effects of inoculating the culture medium of potato microplants grown in vitro with Azospirillum brasilense Sp245 or Ochrobactrum cytisi IPA7.2. Growth and hormone content of the plants were evaluated under stress-free conditions and under a water deficit imposed with polyethylene glycol (PEG 6000). Inoculation with either bacterium promoted the growth in terms of leaf mass accumulation. The effects were associated with increased concentrations of auxin and cytokinin hormones in the leaves and stems and with suppression of an increase in the leaf abscisic acid that PEG treatment otherwise promoted in the potato microplants. O. cytisi IPA7.2 had a greater growth-stimulating effect than A. brasilense Sp245 on stressed plants, while A. brasilense Sp245 was more effective in unstressed plants. The effects were likely to be the result of changes to the plant’s hormonal balance brought about by the bacteria.

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“…The object of research was a spring durum wheat cultivar Nikolasha. The seeds before sowing and plants in the tillering phase were inoculated with a suspension of the bacterial strains Azospirillum brasilense Sp245, Sp7, Cd, SR80 and SR88 [17], Ochrobactrum cytisi IPA7.2 [18], as well as Enterobacter cloacae K7 [19], derived from pure bacterial cultures in the logarithmic growth phase. The cell concentration in suspension was 10 8 in 1 ml.…”

Section: Methodsmentioning

confidence: 99%

Study of the effect of associative rhizobacterial strains on the formation of spring durum wheat productivity

et al. 2020

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The article considers the influence of strains of associative rhizospheric growth-promoting bacteria on the growth and productivity of spring durum wheat. Seeds before sowing and plants in the tillering phase were inoculated with a suspension of rhizospheric bacteria Azospirillum brasilense of strains Sp245, Sp7, Cd, SR80 and SR88, Ochrobactrum cytisi IPA7.2, Enterobacter cloacae K7. Significant differences were observed in terms of productivity of inoculated wheat plants in comparison with the control. Two strains of bacteria A brasilense SR80, SR88 promoted plant growth. Strains of rhizospheric bacteria A. brasilense Sp245, Sp7, SR80, SR88, and E. cloacae K7 under arid conditions of the Volga’s Left Bank of the Saratov Region contributed to an increase in the photo synthetic surface of leaves and the productivity of photosynthesis, which affected the formation of spring durum wheat productivity. Strains of rhizospheric bacteria A brasilense Sp245 and Sp7 positively affected the grain quality. Agrobiotechnology based on associative microsymbionts can be used to optimize environmentally sound technologies for the production of durum wheat (Triticum durum Desf.) in the organic farming system.

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Ochrobactrum cytisi IPA7.2 promotes growth of potato microplants and is resistant to abiotic stress

Cited by 28 publications

References 60 publications

Taxonomic Organization of the Family Brucellaceae Based on a Phylogenomic Approach

Taxonomic Organization of the Family Brucellaceae Based on a Phylogenomic Approach

Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress

Study of the effect of associative rhizobacterial strains on the formation of spring durum wheat productivity

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