Ocorrência das biovares 1 e 2 de Ralstonia solanacearum em lavouras de batata no estado do Rio Grande do Sul

Silveira, José Ricardo Pfeifer; Duarte, Valmir; Moraes, Marcelo Gravina de

doi:10.1590/s0100-41582002000500002

Cited by 7 publications

(12 citation statements)

References 3 publications

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“…Trials done in Rio Grande do Sul (RS), for instance, between 1997 and 1999, characterized 94% and 6% of R. solanacearum isolates as R3Bv2 and R1Bv1, respectively, in different potato cultivars and planting seasons (MACIEL et al, 2001;MORAES, 2002). High frequency of R3Bv2 was expected and confirms the hypothesis of prevalence of this biovar in that State, probably due to milder temperatures (14 to 22 o C) during the major growing season.…”

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 66%

“…In turn, the occurrence of R1Bv1 in spring crops indicates that late plantings are less favorable to that biovar. However, in tomato, eggplant, and tobacco grown in the same State, the predominance of R1Bv1 was identified (SILVEIRA et al, 2006). These results demonstrate that climate and soil conditions in RS allow the occurrence of both biovars, with the predominance of one of them determined by the host plant and planting season.…”

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 98%

“…Subsequently, its occurrence was reported in potato in South of Brazil, although in lower frequency than R3Bv2 (LOPES; NAZARENO;FURIATTI, 1993;MACIEL et al, 2001;MORAES, 2002;SILVEIRA et al, 2005). Moreover, under special conditions of moisture and temperature, it has caused significant losses in commercial eucalypt nurseries in the States of Espírito Santo, Santa Catarina, Maranhão, Minas Gerais, Bahia, Pará, and Goiás (ALFENAS et al, 2006;AUER;.…”

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 99%

“…It is most commonly found, with no exclusivity, in crops in the South and Southeast, where most of the potato is grown in country (FRENCH et al, 1993;NAZARENO;FURIATTI, 1993;MACIEL et al, 2001;MORAES, 2002). Besides affecting potato crops, this race was found associated to geranium (Pelargonium zonale) in the State of São Paulo (ALMEIDA et al, 2003).…”

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 99%

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Occurrence and diversity of Ralstonia solanacearum populations in Brazil

et al. 2015

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ABSTRACT:Ralstonia solanacearum is a gram-negative soil-borne bacterium capable of infection of hundreds of vegetable species over more than 50 botanical families, causing bacterial wilt, except for bananas, when the disease is called Moko. It deserves special attention, from all other plant pathogenic bacteria, for its high phenotypic and genotypic plasticity, a characteristic that makes disease control extremely difficult. In this context, frequent and necessary surveys are conduct in the attempt of characterizing the prevailing strains of R. solanacearum in each region where the disease has been reported. However, knowledge about occurrence and diversity of R. solanacearum in Brazil is fragmented and in some cases, based on inconclusive studies with few strains, little representative of a given region. The need to obtain a greater picture guided this review. The occurrence of this bacterium in Brazilian States and the possible causes for its dissemination are presented, with emphasis on studies of genetic variability of populations of R. solanacearum in the country. The compiled results report a wide distribution of the bacterium in Brazil and great variability of its populations among locations. Partly due to the difficulty of detecting small titer of bacteria in samples, paucity of information about the origin of inoculum in certain regions is observed, as well as the need for detecting the presence of the pathogen in asymptomatic plants, potato tubers with latent infections, soil, and water, which are the major causes of bacterial dissemination into areas without any disease history.

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Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 66%

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 98%

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 99%

Section: Ralstonia Solanacearum Races and Biovars In Brazilmentioning

confidence: 99%

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Occurrence and diversity of Ralstonia solanacearum populations in Brazil

et al. 2015

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“…Os isolados obtidos foram identificados através dos seguintes testes: gram, atividade pectolítica em batata, oxidase, catalase, fermentação de glicose (oxidação/ fermentação), crescimento a 37°C, utilização de α-metil glicosídeo (estirpes positivas formam colônias com centro vermelho), sensibilidade à eritromicina, produção de substâncias redutoras de sacarose, produção de ácidos a partir de maltose D(+), lactose e sorbitol (Hyman et al, 1998;De Boer & Kelman, 2001 A capacidade dos isolados em utilizar diferentes açúcares foi testada em placas de microtitulação (96 amostras/ placa) (Silveira et al, 2002;Oliveira et al, 2003) contendo 150 µl de meio de cultura (peptona, 10 g; púrpura de bromocresol 1,5%, 0,7 ml; ágar, 0,3 g; açúcar 10%, 100ml/l, pH 6,8) (Dickey & Kelman, 1988). Culturas com 24-48 h de crescimento foram transferidas para o meio com o auxílio de um palito de madeira esterilizado, e incubadas a 28 °C por 48 h. A mudança de cor de púrpura para amarela foi considerada positiva.…”

Section: Identificação Bioquímica E Fisiológicaunclassified

Ocorrência de pectobactérias em tubérculos de batata-semente no estado do Rio Grande do Sul

Tassa

Duarte

2004

Fitopatol. bras.

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Tubérculos de batata (Solanum tuberosum)-semente, pré-básica, básica, registrada e certificada, de oito cultivares, oriundos de 21 lavouras localizadas nos municípios de Vacaria, Canguçu, Piratini e Ibiraiaras, no Rio Grande do Sul, foram coletados nos meses de maio a agosto de 2002. Cada tubérculo foi lavado em água corrente, deixado secar à temperatura ambiente, perfurado com palitos em dez lenticelas, coberto com fina camada de óleo de soja, colocado individualmente em cima de folha de papel toalha umedecida dentro de saco plástico transparente e incubado a 23 ºC por quatro dias. A incidência de podridão mole a partir das lenticelas variou de 20_100% entre as cultivares. Pectobacterium sp. foi constatada em tubérculos das 21 lavouras. Duzentos e vinte e três isolados de Pectobacterium sp. foram obtidos em meio CPG, a partir das lenticelas com podridão mole, e identificados por testes bioquímicos, fisiológicos e PCR em nível de subespécie. Cento e dezenove isolados foram identificados como P. carotovorum subsp. brasiliensis e e 96 com o P. carotovorum subsp. carotovorum. Oito isolados não se enquadraram na classificação bioquímica. Pectobacterium carotovorum subspp. estavam presentes em tubérculos de batata-semente, independente da cultivar, classe ou município de origem. Pectobacterium carotovorum subsp. atrosepticum, a principal responsável por causar canela preta em batata em outros países, não foi detectada.

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Genetic Diversity and Aggressiveness of Ralstonia solanacearum Strains Causing Bacterial Wilt of Potato in Uruguay

2011

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Bacterial wilt, caused by Ralstonia solanacearum, is a major disease affecting potato (Solanum tuberosum) production worldwide. Although local reports suggest that the disease is widespread in Uruguay, characterization of prevalent R. solanacearum strains in that country has not been done. In all, 28 strains of R. solanacearum isolated from major potato-growing areas in Uruguay were evaluated, including 26 strains isolated from potato tubers and 2 from soil samples. All strains belonged to phylotype IIB, sequevar 1 (race 3, biovar 2). Genetic diversity of strains was assessed by repetitive-sequence polymerase chain reaction, which showed that the Uruguayan strains constituted a homogeneous group. In contrast, inoculation of the strains on tomato and potato plants showed, for the first time, different levels of aggressiveness among R. solanacearum strains belonging to phylotype IIB, sequevar 1. Aggressiveness assays were also performed on accessions of S. commersonii, a wild species native to Uruguay that is a source of resistance for potato breeding. No significant interactions were found between bacterial strains and potato and S. commersonii genotypes, and differences in aggressiveness among R. solanacearum strains were consistent with previously identified groups based on tomato and potato inoculations. Moreover, variation in responses to R. solanacearum was observed among the S. commersonii accessions tested.

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Ocorrência das biovares 1 e 2 de Ralstonia solanacearum em lavouras de batata no estado do Rio Grande do Sul

Cited by 7 publications

References 3 publications

Occurrence and diversity of Ralstonia solanacearum populations in Brazil

Occurrence and diversity of Ralstonia solanacearum populations in Brazil

Ocorrência de pectobactérias em tubérculos de batata-semente no estado do Rio Grande do Sul

Genetic Diversity and Aggressiveness of Ralstonia solanacearum Strains Causing Bacterial Wilt of Potato in Uruguay

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