Oct3/4 is potentially useful for the suppression of the proliferation and motility of hepatocellular carcinoma cells

Tomizawa, M.; Shinozaki, Fuminobu; Motoyoshi, Yasufumi; Sugiyama, Takao; Yamamoto, Shozo; Ishige, Naoki

doi:10.3892/ol.2018.9292

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…The regulation of cell proliferation in HLF cells is modulated by different factors like Oct3/4. Indeed, the down-regulation of Oct3 /4 was demonstrated also to regulate proliferation and motility in HLF cells, but not in other hepatocarcinoma cell lines [37], increasing β-catenin protein level with a consequent Wnt signaling amplification, finally enhancing the invasive cellular activity that is characteristic of epithelial-mesenchymal transition [38]. Such features can reasonably explain the behavior in terms of silencing response of HLF compared to the other primary tumor cell lines investigated in this study.…”

Section: Discussionmentioning

confidence: 99%

FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation

Scavo

Depalo

Rizzi

et al. 2019

Cells

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Extracellular vesicles (EVs) are involved in intercellular communication during carcinogenesis, and cancer cells are able to secrete EVs, in particular exosomes containing molecules, that can be transferred to recipient cells to induce pathological processes and significant modifications, as metastasis, increase of proliferation, and carcinogenesis evolution. FZD proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in carcinogenesis of the gastroenteric tract. Here, a still unknown role of Frizzled 10 (FZD10) protein was identified. In particular, the presence of FZD10 and FZD10-mRNA in exosomes extracted from culture medium of the untreated colorectal, gastric, hepatic, and cholangio cancer cell lines, was detected. A substantial reduction in the FZD10 and FZD10-mRNA level was achieved in FZD10-mRNA silenced cells and in their corresponding exosomes. Concomitantly, a significant decrease in viability of the silenced cells compared to their respective controls was observed. Notably, the incubation of silenced cells with the exosomes extracted from culture medium of the same untreated cells promoted the restoration of the cell viability and, also, of the FZD10 and FZD10-mRNA level, thus indicating that the FZD10 and FZD10-mRNA delivering exosomes may be potential messengers of cancer reactivation and play an active role in long-distance metastatization.

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Section: Discussionmentioning

confidence: 99%

FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation

Scavo

Depalo

Rizzi

et al. 2019

Cells

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“…The present study was performed using renal cell lines. Although it has been reported that Oct3/4 knockdown suppressed the proliferation of hepatocellular carcinoma cells in the condition of 3-day incubation (Tomizawa et al, 2018), whether suppressed proliferation restore by longer incubation is remained to be unknown. In fact, in the present study, 3-day incubation of ACHN cells with LXR inverse agonist caused a decrease in cell number, however, the cell number was restored after 4 days (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…Oct3/4 is also involved in cancer cell growth. For example, Oct3/4 knockdown suppresses the proliferation of hepatocellular carcinoma cells (Tomizawa et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Farnesoid X receptor and liver X receptors regulate Oct3/4 expression by multiple feedback regulating system in normal renal-derived cells and renal adenocarcinoma cells

et al. 2020

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In this study, we found that nuclear receptors FXR and LXR (originally characterized as regulatory factors involved in cholesterol/bile acid homeostasis) regulate the expression of Oct3/4, a marker for cell differentiation, in both normal renal-derived cell line HK-2 and renal adenocarcinoma cell line ACHN. Down-regulation of Oct3/4 expression by activating FXR and LXR occurs only in normal renal cell-derived HK-2 cells. We also found that the RNA-binding protein, ELAVL2, oppositely regulates Oct3/4 expressions in HK-2 and ACHN cells. Moreover, we revealed that LXR-alpha and LXR-beta regulate each other's expression. Although an LXR-beta-specific agonist is assumed to be the basis for an anti-arteriosclerotic drug that only stimulates reverse cholesterol transport, our findings show that the development of such an anti-arteriosclerotic drug would require further elucidation of the complex mechanism of LXR-alpha and LXR-beta regulation.

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The Haematopoietically-expressed homeobox transcription factor: roles in development, physiology and disease

2023

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The Haematopoietically expressed homeobox transcription factor (Hhex) is a transcriptional repressor that is of fundamental importance across species, as evident by its evolutionary conservation spanning fish, amphibians, birds, mice and humans. Indeed, Hhex maintains its vital functions throughout the lifespan of the organism, beginning in the oocyte, through fundamental stages of embryogenesis in the foregut endoderm. The endodermal development driven by Hhex gives rise to endocrine organs such as the pancreas in a process which is likely linked to its role as a risk factor in diabetes and pancreatic disorders. Hhex is also required for the normal development of the bile duct and liver, the latter also importantly being the initial site of haematopoiesis. These haematopoietic origins are governed by Hhex, leading to its crucial later roles in definitive haematopoietic stem cell (HSC) self-renewal, lymphopoiesis and haematological malignancy. Hhex is also necessary for the developing forebrain and thyroid gland, with this reliance on Hhex evident in its role in endocrine disorders later in life including a potential role in Alzheimer’s disease. Thus, the roles of Hhex in embryological development throughout evolution appear to be linked to its later roles in a variety of disease processes.

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Oct3/4 is potentially useful for the suppression of the proliferation and motility of hepatocellular carcinoma cells

Cited by 5 publications

References 23 publications

FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation

FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation

Farnesoid X receptor and liver X receptors regulate Oct3/4 expression by multiple feedback regulating system in normal renal-derived cells and renal adenocarcinoma cells

The Haematopoietically-expressed homeobox transcription factor: roles in development, physiology and disease

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