Ocular Burn: Rinsing and Healing with Ionic Marine Solutions and Vegetable Oils

Abstract

Purpose: We investigated the effects of various rinsing and healing protocols on corneal wound repair and inflammation following alkali burn in rabbits. Methods: We conducted in vitro, in vivo and ex vivo studies. First, different rinse solutions were tested in vitro after incubation of ocular cells with methanol or NaOH. Cell viability was then assessed using the neutral red test (cytofluorometry). Second, NaOH was applied to rabbit corneas and associations of rinse solutions (NaCl 0.9% or controlled ionization marine solutions) with N-acetylcysteine or vegetable oils (from Calophyllum inophyllum and Aleurites moluccana) were tested in vivo. The regeneration of the corneal epithelium and the infiltration of inflammatory cells were evaluated using in vivo confocal microscopy and ex vivo histological cuts. Results: The association of a controlled ionization marine solution with 10% C. inophyllum oil and 90% A. moluccana oil induced regeneration of the corneal epithelium and a decrease in inflammatory cells. Conclusions: Irrigation with marine solution followed by treatment with a mixture of C. inophyllum and A. moluccana oils is a promising treatment for ocular burns.

Ocular cytotoxicity evaluation of medical devices such as contact lens solutions and benefits of a rinse step in cleaning procedure

“…As it induced cell death as soon as 30 minutes, we also evaluated the benefits of a rinse step after use of this solution and observed that unpreserved saline solutions again avoided cytotoxicity. In each case, the controlled ionization controlled marine solution was more efficient than NaCl 0.9% to rinse contact lenses, which is in accordance with previous results (Dutot et al, 2010;Said et al, 2009). Oligoelements and ions are already known to exert beneficial ocular effects (Dutot et al, 2008b).…”

Multipurpose Solutions and Contact Lens: Modulation of Cytotoxicity and Apoptosis on the Ocular Surface

“…This cationic marine solution was chosen because of its effectiveness in rinsing chemicals away in a model of ocular burn and in modulating P2X7 cell death receptor activation. 8,9 The rise in the use of MPS has been accompanied by substantially more reports of solution-related complications, in particular corneal staining and inflammatory infiltrates. Most MPS manufacturers recommend rinsing the lenses with the multipurpose solution before insertion of the lens.…”

“…The marine solution was chosen according to our previous findings that showed its ability to decrease P2X7 receptor activation (which is induced by MPS) and to rinse ocular cells after chemical burn in a better way than NaCl 0.9%. 8,9 In a previous study, hyaluronic acid showed cytoprotective effects against preservative cytotoxicity. 10 Because active principles in MPS are preservatives, we studied the protective effects of hyaluronic acid against MPS cytotoxicity.…”

Antinociceptive, anti-inflammatory and wound healing features in animal models treated with a semisolid herbal medicine based on Aleurites moluccana L. Willd. Euforbiaceae standardized leaf extract

“…This activity, together with the anti-inflammatory effects presented above, may represent primordial factors for the wound contraction and healing process acceleration evidenced in this study. Furthermore, Said and co-authors (Said et al, 2007(Said et al, , , 2009) recently demonstrated that A. moluccana essential oil was capable of conferring ocular cytoprotection, with corneal epithelium regeneration and reduction of inflammatory cell infiltration.…”