Ocular imaging of Carassius auratus eyes using photoacoustic microscopy

Samanta, Deepayan; Mathew, Arunima; Paul, Souradip; Paramanick, Arijit; Aiswarya, K S; Singh, Mayanglambam Suheshkumar

doi:10.1117/12.2650325

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…It provides images with sufficient depth, good resolution, and high optical contrast. [7][8][9][10][11][12][13] PAI relies on the photoacoustic (PA) effect, and the combined ultrasound and optical properties offer insights into the structural, functional, and molecular information of tissue. [14][15][16][17] A conventional PAI system is relatively expensive and mechanically bulky.…”

Section: Introductionmentioning

confidence: 99%

Simulation of photoacoustic signal detection using fiber Bragg gratings

Paramanick,

Shirin,

Samanta

et al. 2024

Photons Plus Ultrasound: Imaging and Sensing 2024

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Photoacoustic imaging (PAI) is now a very promising medical imaging technology that provides structural, functional, and molecular information based on the optical absorption of endogenous or exogenous contrast agents. A conventional PAI system using an array of ultrasonic transducers to detect photoacoustic (PA) signals. Each element of the transducer requires an amplifier to enhance the detected signal which makes it bulky. To overcome this problem, we introduced optical sensor alternative to piezoelectric ultrasound detector in PA signal detection. This study aims to illustrate the entire process of PA signal generation and its detection using an optical sensor using Finite Element software (COMSOL). Fiber Bragg grating (FBG) is one of the optical sensors that may be utilized in some applications in place of acoustic ones. FBG provides the change in acoustic pressure as a function of the shift of Bragg wavelength. Our study demonstrated that FBG achieves to detect photoacoustic (PA) signals as an alternative of US transducer, with superior performance in terms of sensitivity, and being more light-weight, flexible, and cost-effective. This implies that these technologically novel qualities hold promise for the use of FBG (either single or multiple units on a single fiber) as an acoustic sensor in PAI systems in place of the conventional piezoelectric-based bulk array transducer. An intensity-based demodulation was performed to extract the US signal. The preliminary study demonstrates that the integration of FBG in PA imaging modality offers potential as a future (imaging) technology both for biological studies and clinical applications.

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Section: Introductionmentioning

confidence: 99%

Simulation of photoacoustic signal detection using fiber Bragg gratings

Paramanick,

Shirin,

Samanta

et al. 2024

Photons Plus Ultrasound: Imaging and Sensing 2024

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“…The NO production by microglia was captured by novel inhouse probe which turn on fluorescent signal after reacting with NO. The efficacy of NO detection and specificity of probe was characterized in microglia treated with LPS, IFNγ and SARS-CoV-2 RNA[27,28].…”

mentioning

confidence: 99%

G6PD deficiency mediated impairment of iNOS and lysosomal acidification affecting phagocytotic clearance in microglia in response to SARS-CoV-2

Mondal,

Munan,

Saxena

et al. 2023

Preprint

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The glucose-6-phosphate dehydrogenase (G6PD) deficiency is X-linked and is the most common enzymatic deficiency disorder globally. It is a crucial enzyme for the pentose phosphate pathway and produces NADPH, which plays a vital role in the regulation of oxidative stress of many cell types. The deficiency of G6PD causes hemolytic anemia, diabetes, cardiovascular and neurological disorders. Notably, the patient with G6PD deficiency was severely affected by SARS-CoV-2 and showed prolonged COVID-19 symptoms, neurological impacts, and high mortality. However, the mechanism of COVID-19 severity in G6PD deficient patients is still ambiguous. Here, using a CRISPR-edited G6PD deficient human microglia cell culture model, we observed a significant reduction in NADPH and an increase in basal reactive oxygen species (ROS) in microglia. Interestingly, the deficiency of the G6PD-NAPDH axis impairs induced nitric oxide synthase (iNOS) mediated nitric oxide (NO) production which plays a fundamental role in inhibiting viral replication. Surprisingly, we also observed that the deficiency of the G6PD-NADPH axis reduced lysosomal acidification, which further abrogates the lysosomal clearance of viral particles. Thus, impairment of NO production and lysosomal acidification as well as redox dysregulation in G6PD deficient microglia altered innate immune response, promoting the severity of SARS-CoV-2 pathogenesis.

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Ocular imaging of Carassius auratus eyes using photoacoustic microscopy

Cited by 2 publications

References 11 publications

Simulation of photoacoustic signal detection using fiber Bragg gratings

Simulation of photoacoustic signal detection using fiber Bragg gratings

G6PD deficiency mediated impairment of iNOS and lysosomal acidification affecting phagocytotic clearance in microglia in response to SARS-CoV-2

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