Ocular surface upregulation of intercellular adhesive molecule-1 (ICAM-1) by local interferon-gamma (IFN-γ) in the rat

Abstract

Inflammatory mediators such as interferon-gamma (IFN-gamma) are known to induce the expression of class II HLA (HLA-DR) and intercellular adhesion molecule 1 (ICAM-1) in a variety of cell types including epithelial cells. The coexpression of ICAM-1 and HLA-DR has been implicated in the pathogenesis of immune mediated ocular disease. We investigated the expression of ICAM-1 on the ocular surface of the rat eye following subconjunctival administration of IFN-gamma. A dose response study was performed using 100, 1,000 and 10,000 IU IFN-gamma/dose. The presence of ICAM-1 was determined using a standard immuno-peroxidase staining technique. The cornea, limbus, and conjunctiva were evaluated. The degree of reaction product in each area was graded by a masked observer. We found constitutive expression of ICAM-1 on the conjunctiva but not on the cornea. There was a significant relationship between the dose of IFN-gamma and the intensity of ICAM-1 staining on the conjunctiva (p < or = 0.05) and on the limbus (p < or = 0.04). Subconjunctival IFN-gamma had no effect on the expression of ICAM-1 in the cornea.

ICAM-1 Is Necessary for Epithelial Recruitment of γδ T Cells and Efficient Corneal Wound Healing

“…It appears that ICAM-1 is not critical to their migration from the vasculature into the stroma. Corneal epithelial cells and limbal vessel endothelial cells can express ICAM-1 14 -21 in response to inflammatory cytokines 35,36 such as tumor necrosis factor and interleukin-1␤ (both expressed in our model of epithelial abrasion). While ICAM-1 is known to contribute to lymphocyte adhesion and transendothelial migration, other adhesion molecules (eg, VCAM-1) also support lymphocyte transmigration.…”

Age-related Defects in Ocular and Nasal Mucosal Immune System and the Immunopathology of Dry Eye Disease

“…91,258,259 The integrated nature of OMIS and NALT systems may be important for ocular surface inflammation and inflammatory DED. 91,93,245,259–262 Topically administrated solutions to the ocular surface are conducted by NLDs into the inferior meatus of the nose, where it reaches the NALT system.…”

Nasolacrimal Duct Closure Modulates Ocular Mucosal and Systemic CD4+T-Cell Responses Induced following Topical Ocular or Intranasal Immunization

“…For immunization purposes, we used a cocktail of four peptides rather than a single peptide. Previously it was shown that in mice, immunization with a mixture of 4 gD peptides induced better T-cell-mediated protective immunity than any individual peptide (5,6). This information could be advantageous for testing of vaccine efficacy with outbred populations, such as rabbits and humans.…”

“…The vascular nature of the OMIS (both circulatory and lymphatic systems) is involved in the process of aqueous outflow and lacrimal gland/duct drainage. The anatomical connection between the OMIS and NALT suggests an immunological connection and interdependency (6,10,35). The integrated nature of the OMIS and NALT systems may be important for ocular immunoprophylactic and immunotherapeutic vaccine development (5,10,(34)(35)(36)(37)39).…”