Aims: To measure the orbital volume of normal Korean people in two different age groups (subjects were in their 20s or 40s), and analyze the differences of orbital volume with respect to age and gender. In addition, to analyze correlation between body parameters (height and weight) and the orbital volume. Methods: Magnetic resonance imaging (MRI) data were acquired for a total of 143 subjects, consisting of 71 subjects in their 20s (32 males and 39 females) and 72 subjects in their 40s (30 males and 42 females). Two-way ANOVA was used to analyze how orbital volume changes with respect to gender and age. A multiple regression analysis was performed to investigate the correlation between body parameters and the orbital volume. Results: The orbital volume of subjects in their 20s was larger than that of subjects in their 40s, and the volume was larger in men than in women. As age increased, the decrease in the orbital volume of women was greater than that of men. While weight and height showed positive correlations with orbital volume in male and female subjects in their 20s, respectively, weight showed a positive correlation with orbital volume in male and female subjects in their 40s. Conclusions: These results provide basic information about the effect of age, gender, and body parameters on orbital volume of Korean people in their 20s or 40s.