Odd-even effects on hydration of natural polyelectrolyte multilayers: An in situ synchrotron FTIR microspectroscopy study

Benbow, Natalie L.; Webber, Jessie L.; Pawliszak, Piotr; Sebben, Damien A.; Karpiniec, Sam; Stringer, Damien N.; Tobin, Mark J.; Vongsvivut, Jitraporn; Krasowska, Marta; Beattie, David A.

doi:10.1016/j.jcis.2019.06.036

Cited by 14 publications

(11 citation statements)

References 80 publications

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“…Interestingly, the slopes of the ΔD−ΔF profiles in the second zone correspond to BNC adsorption. This was comparable in all cases, which confirms the adsorption mechanism of the BNC in PEI by in situ layer-by-layer built up was a proper method to form a monolayer film in the sensor [ 31 ]. It can be clearly observed that the intensity (changed from quantity) of adsorption (as determined by the maximum ΔF) was more limited for B12, followed by B2, B1, and B3 (ca.…”

Section: Resultssupporting

confidence: 63%

Surface Interactions between Bacterial Nanocellulose and B-Complex Vitamins

et al. 2020

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The interactions between films of bacterial nanocellulose (BNC) and B complex vitamins were studied using a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D). Thin films of BNC were generated in situ by QCM-D, followed by real-time measurements of the vitamin adsorption. The desorption of vitamins was induced by rinsing the system using phosphate buffers at a pH of 2 and 6.5, emulating gastric conditions. Changes in frequency (which are proportional to changes in adsorbed mass, ∆m) detected by QCM-D were used to determine the amounts of vitamin adsorbed and released from the BNC film. Additionally, changes in dissipation (∆D) were proven to be useful in identifying the effects of the pH in both pristine cellulose films and films with vitamin pre-adsorbed, following its changes during release. The effects of pH on the morphology of the vitamin-BNC surfaces were also monitored by changes in rugosity from images obtained by atomic force microscopy (AFM). Based on this data, we propose a model for the binding phenomena, with the contraction on the relaxation of the cellulose film depending on pH, resulting in an efficient vitamin delivery process.

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Section: Resultssupporting

confidence: 63%

Surface Interactions between Bacterial Nanocellulose and B-Complex Vitamins

et al. 2020

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“…Both films (with and without nisin Z) display supra-linear growth. This type of growth is commonly observed for natural polysaccharide polymers, which are more likely to experience greater polydispersity, as well as diffusion of adsorbing species into the bulk of the film 39,40 .…”

Section: Resultsmentioning

confidence: 69%

“…Its incorporation and the formation of the multilayers both with and without nisin Z were investigated using several techniques. These techniques include the in situ technique of attenuated total reflection Fourier transform infrared spectroscopy, ATR FTIR, used previously by our group to characterise PEMs 39,40 . In addition, the thickness of the formed layers was measured and the surface topography and wettability assessed, as these parameters can influence bacterial cell adhesion onto surfaces, and thus can play a role in how the films interact with bacterial cells 41 .…”

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confidence: 99%

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Webber

Namivandi-Zangeneh

Drozdek

et al. 2021

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An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm−2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.

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“…S3 †), similar to that observed in other LbL systems. [85][86][87][88] This behaviour is related to the reported effect of polysaccharides on protein-tannic acid interactions. [77][78][79][80] It may be attributed to the changing charge density at the surface as subsequent layers are deposited, and can be avoided by using very high ionic strength in the deposition solutions, something not wanted in the present experiments in view of the subsequent emulsion application.…”

Section: Layer-by-layer Work Uv-vis and Pm-irrasmentioning

confidence: 67%

“…It also shows that the deposition is not entirely linear, although for such a small number of layers it is impossible to tell if it is eventually becomes exponential as in other systems. 86,88,89 The results of Fig. 5 indicate that chitosan and pectin should be preferable for emulsion work over chondroitin sulfate, and sodium alginate.…”

Section: Layer-by-layer Work Uv-vis and Pm-irrasmentioning

confidence: 93%