Odontogenic differentiation of inflamed dental pulp stem cells (IDPSCs) on polycaprolactone (PCL) nanofiber blended with hydroxyapatite

Abstract

The objective of this study was to develop electrospun polycaprolactone (PCL) membranes blended with hydroxyapatite (HA) and evaluate its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts. Electrospun nanofibrous membrane consisting of PCL blended with 10 wt% and 15 wt% of HA were fabricated and the characterization was done by Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and contact angle analysis. Cytocompatibility, cell adhesion and odontogenic differentiation ability of the membranes were assessed by MTT, Live/ Dead, SEM/DAPI and qPCR studies. The mineral deposition ability of the membranes with IDPSCs was estimated by SEM-EDS. The SEM analysis revealed a nanofibrous texture with an average fiber diameter of 140 nm for PCL, 220 nm for PCL10%HA and 250 nm for PCL15%HA. Among the membranes tested, PCL10%HA favored positive cell attachments, upregulated expression of DSPP and ALP gene and higher Ca/P ratio compared to PCL and PCL15%HA.

Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold

“…The other is the biological functions of hydroxyapatite coated on PCL. A large number of studies have now demonstrated the intrinsic ability of hydroxyapatite to promote osteogenic differentiation of stem cells in vitro [ 21 , 40 , 41 , 42 , 43 ]. It has been suggested that the nanostructured hydroxyapatite can induce the osteogenic differentiation of stem cells and form the new bone through in vivo bio-mineralization by the release of ions (Ca 2+ and PO 4 3 − ) by degradation [ 44 ].…”

Electrospun nanofibers applications in caries lesions: prevention, treatment and regeneration

“…Several scholars have investigated DPSCs' odontoblastic differentiation on PCL or PCL/PLA electrospun scaffolds with the addition of different active substances. Their choices of dopants included nanohydroxyapatite (nHAP), [83][84][85] zeolite, 86 calcium hydroxide, and fibronectin. 87 On each of these composite scaffolds, DPSCs showed superior proliferation and odontoblastic differentiation behavior than on untreated scaffolds.…”

Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

“…Polycaprolactone/gelatin/hyaluronic acid nanofibers were developed to mimic a glioblastoma tumor extracellular matrix [ 152 ]. In another study, electrospun PCL membranes blended with hydroxyapatite (HA) were developed, and its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts was evaluated [ 180 ]. The results showed that fluorapatite coating on the electrospun PCL nanofiber surface facilitated adhesion, proliferation, and differentiation of DPSCs to odontoblasts and might be used as a tool in the therapy of bone damage [ 180 ].…”

“…In another study, electrospun PCL membranes blended with hydroxyapatite (HA) were developed, and its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts was evaluated [ 180 ]. The results showed that fluorapatite coating on the electrospun PCL nanofiber surface facilitated adhesion, proliferation, and differentiation of DPSCs to odontoblasts and might be used as a tool in the therapy of bone damage [ 180 ]. Another artificial biomaterial, polyhydroxybutyrate (PHB)/chitosan/nanobioglass (nBG) nanofiber scaffold with seeded human exfoliated deciduous stem cells (SHEDs), has been tested in regenerative dentistry [ 181 ].…”