Odontogenic effects of two calcium silicate-based biomaterials
in human dental pulp cells

Onay, Emel Olga; Yurtçu, Erkan; Terzi, Yunus Kasım; Üngör, Mete; Oğuz, Yener; Şahin, Feride İffet

doi:10.17219/acem/74197

Cited by 9 publications

(11 citation statements)

References 25 publications

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“…This could suggest a reaction to the implantation of BD, indicating its mineralizing potential, as in cytokine measurements in the present study, this material significantly expressed IL‐8 at 30 days and also expressed mRNA of BMP2 in the same period. As for MTA and BMP2, our study confirmed the findings of Önay et al, 27 but Silva et al 28 showed that MTA stimulates BMP2 expression. This suggests that these affinities could result from the close relationship of BMPs with osteoblasts, where both BMP2 and BMP4 have effects on osteoblasts, 73 making it difficult to elucidate the specific roles during osteogenesis.…”

Section: Discussionsupporting

confidence: 91%

“…In addition, only one study to date has assessed the relationship between BMP4 and MTA, thus confirming its osteogenic‐odontogenic potential 26 . As for BMP2, a study has demonstrated no relationship between BMP2 and MTA, only in a special formulation known as MM‐MTA 27 ; however, another study has shown that MTA stimulates BMP2 expression 28 …”

Section: Introductionmentioning

confidence: 99%

“…24,25 In addition, only one study to date has assessed the relationship between BMP4 and MTA, thus confirming its osteogenic-odontogenic potential. 26 As for BMP2, a study has demonstrated no relationship between BMP2 and MTA, only in a special formulation known as MM-MTA 27 ; however, another study has shown that MTA stimulates BMP2 expression. 28 Accordingly, the aim of the present study is to assess the role of MTA and BD in BMPs and inflammatory cytokines to elucidate the mechanisms whereby these materials induce mineralization and to confirm their biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Bone morphogenetic proteins in biomineralization of two endodontic restorative cements

et al. 2020

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To assess the effect of biodentine (BD) and MTA‐angelus (MTA) on biocompatibility, BMP2, BMP4, and osteocalcin (OC) expression. Subcutaneously implanted tubes of four groups (MTA, BD, Control, and Sham) were kept over 15, 30, and 60 days; histological analyses were performed using H&E and Von Kossa; ELISA quantified IL‐1β and IL‐8 expression; and qRT‐PCR verified gene expression of BMPs and OC. Sham showed slight changes in profile/intensity of inflammatory infiltrate in all periods. Control had an inflammatory score significantly higher than Sham at 15 days (p < .05). BD revealed a similar inflammatory response to Sham, without significant changes over periods. MTA group exhibited an increase in chronic inflammatory profile at 30 days, with significant reduction at 60 days, when compared to Sham (p < .05). At 30/60 days, experimental groups presented birefringent areas. At 30/60 days, BD and MTA significantly increase IL‐1β compared to Control, whereas an increase in IL‐8 was observed only in BD. At 30/60 days, BD produces an expression of BMP2 whereas MTA influenced BMP4 and OC. Materials tested are biocompatible and they have osteoinductive activity; the materials influenced the expression of the tested mediators differently, suggesting different affinities with the substrate and the dental substrates.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bone morphogenetic proteins in biomineralization of two endodontic restorative cements

et al. 2020

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“…A similar inflammatory effect was also observed for all materials, in the protein expression of inducible NO synthase and cyclooxygenase‐2 (COX‐2) as well as the expressions of pro‐inflammatory cytokines tumour necrosis factor alpha, interleukin (IL)‐1b, IL‐6 and IL‐8 (Chang et al, 2015). In another study, HDPCs were in indirect contact with MM‐MTA or ProRoot MTA extracts at 1, 7 and 14 days (Önay et al, 2018). Both materials exhibited similar expression levels of odontogenic markers for instance alkaline phosphatase liver/bone/kidney (ALPL), DSPP, runx‐related transcription factor 2 (RUNX2) and distal‐less homeobox 3 (DLX3; Önay et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Present status and future directions: Hydraulic materials for endodontic use

Camilleri

Atmeh

et al. 2022

Int Endodontic J

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Background Hydraulic materials are used in Endodontics due to their hydration characteristics namely the formation of calcium hydroxide when mixing with water and also because of their hydraulic properties. These materials are presented in various consistencies and delivery methods. They are composed primarily of tricalcium and dicalcium silicate, and also include a radiopacifier, additives and an aqueous or a non‐aqueous vehicle. Only materials whose primary reaction is with water can be classified as hydraulic. Objectives Review of the classification of hydraulic materials by Camilleri and the literature pertaining to specific uses of hydraulic cements in endodontics namely intra‐coronal, intra‐radicular and extra‐radicular. Review of the literature on the material properties linked to specific uses providing the current status of these materials after which future trends and gaps in knowledge could be identified. Methods The literature was reviewed using PUBMED, and for each clinical use, the in vitro properties such as physical, chemical, biological and antimicrobial characteristics and clinical data were extracted and evaluated. Results A large number of publications were retrieved for each clinical use and these were grouped depending on the property type being investigated. Conclusions The hydraulic cements have made a difference in clinical outcomes. The main shortcoming is the poor testing methodologies employed which provide very limited information and also inhibits adequate clinical translation. Furthermore, the clinical protocols need to be updated to enable the materials to be employed effectively.

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“…e osteodifferentiation of MM-MTA related to increase in alkaline phosphatase activity [77] and the expression of bone morphogenetic protein 2 (BMP2) more than other generations of MTA, and this may be associated with higher concentration of magnesium and sodium [78]. e presence of higher silicon ions in BD stimulates young bone formation by stimulating osteoblasts and promoting mineralization [11].…”

Section: Parametersmentioning

confidence: 99%

The Response of the Pulp-Dentine Complex, PDL, and Bone to Three Calcium Silicate-Based Cements: A Histological Study in an Animal Rat Model

Talabani

Garib

Masaeli

2020

Bioinorganic Chemistry and Applications

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Objective. The aim of this study was to histologically examine the tissue reaction of three different calcium silicate cements in the closure of perforations in rat incisor teeth. Material and Methods. An experimental lateral root perforation with pulp exposure was performed in 32 lower incisors of 16 male Wistar albino rats. They were randomly assigned into three test groups (each including eight teeth) that were filled either by Biodentine (BD) or MicroMega mineral trioxide aggregate (MM-MTA) or EndoSequence root repair material putty (ESRRM putty), besides eight unperforated incisors from the other four rats (control group). The inflammatory response and healing process were evaluated histologically and scored after one and four weeks. Differences among groups were tested by Kruskal–Wallis tests at P≤0.05. Results. In the first week, BD produced more inflammatory response in the pulpal (score 3) than other materials (score 2). Only ESRRM putty showed odontoblast-like cells in 50%, 25% dentine-like deposit, 25% evidence of bone deposition in the drilling site (score 2), and minimum periodontal ligament (PDL) necrosis and disorganization (25%, score 2). After one month, all groups had healthy pulpal tissue, but 25% of ESRRM putty retained score 1 inflammatory response, and 50% of the BD case had an incomplete palisading odontoblast layer (score 3). A thick and regular dentine bridge deposition was seen in the ESRRM putty group in comparison with MM-MTA and BD cases. The cortical plate healing in all ESRRM putty samples was complete (score 3), while an incomplete closure was seen in MM-MTA and BD groups (score 2). Both the MM-MTA and ESRRM putty groups had fully organized PDL (score 2), while in 50% of BD cases, a necrotizing area and disorganized PDL with inflammatory cells infiltration were still present. Statistically significant differences in the scores of any histologic parameters among the three tested materials were observed neither in the 1st nor in the 4th weeks of the experimental period. Conclusion. Better tissue compatibility and repair of pulpal and periodontal tissue have been detected after lateral perforation in the root of rat incisors when treated with ESRRM putty than MM-MTA and BD. However, the difference was not significant.

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Odontogenic effects of two calcium silicate-based biomaterials in human dental pulp cells

Abstract: Based on the cell responses observed in this study, MM-MTA might be used efficiently in dental pulp therapy as a potential alternative to MTA.

Cited by 9 publications

References 25 publications

Bone morphogenetic proteins in biomineralization of two endodontic restorative cements

Bone morphogenetic proteins in biomineralization of two endodontic restorative cements

Present status and future directions: Hydraulic materials for endodontic use

The Response of the Pulp-Dentine Complex, PDL, and Bone to Three Calcium Silicate-Based Cements: A Histological Study in an Animal Rat Model

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