OEM—A new medium for rapid isolation of onion-pathogenic and onion-associated bacteria

Zaid, A. M.; Bonasera, Jean M.; Beer, Steven V.

doi:10.1016/j.mimet.2012.09.031

Cited by 22 publications

(7 citation statements)

References 30 publications

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“…For onion plants from the field, roots were trimmed and plants were rinsed with distilled water to remove soil particles. Symptomatic tissues or disease margins were probed with sterile wooden applicators and streaked onto onion extract medium (OEM) (Zaid et al ) directly. When dealing with bulbs, they were bisected longitudinally, photographed and assessed for symptoms, and bacterial isolations were made from each bulb.…”

Section: Methodsmentioning

confidence: 99%

Rahnella spp. are commonly isolated from onion ( Allium cepa ) bulbs and are weakly pathogenic

et al. 2019

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Aims: Bacterial decays of onion bulbs have serious economic consequences for growers, but the aetiologies of these diseases are often unclear. We aimed to determine the role of Rahnella, which we commonly isolated from bulbs in the United States and Norway, in onion disease. Methods and Results: Isolated bacteria were identified by sequencing of housekeeping genes and/or fatty acid methyl ester analysis. A subset of Rahnella spp. strains was also assessed by multilocus sequence analysis (MLSA); most onion strains belonged to two clades that appear closely related to R. aquatilis. All tested strains from both countries caused mild symptoms in onion bulbs but not leaves. Polymerase chain reaction primers were designed and tested against strains from known species of Rahnella. Amplicons were produced from strains of R. aquatilis, R. victoriana, R. variigena, R. inusitata and R. bruchi, and from one of the two strains of R. woolbedingensis. Conclusions: Based on binational testing, strains of Rahnella are commonly associated with onions, and they are capable of causing mild symptoms in bulbs. Significance and Impact of the Study: While Rahnella strains are commonly found within field-grown onions and they are able to cause mild symptoms, the economic impact of Rahnella-associated symptoms remains unclear.

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Section: Methodsmentioning

confidence: 99%

Rahnella spp. are commonly isolated from onion ( Allium cepa ) bulbs and are weakly pathogenic

et al. 2019

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“…Sampel dibilas dengan air steril dan dikeringkan dengan tissue steril. Air bilasan terakhir diinokulasikan kedalam media Potato Dextrose Agar (M096 Himedia) yang digunakan sebagai kontrol untuk meyakinkan bahwa sterilisasi permukaan tanaman sudah benar dan bakteri yang diisolasi adalah bakteri endofit (Zaid et al 2012).…”

Section: Metode Isolasi Bakteri Endofitunclassified

Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

Wandita

Pujiyanto

Suprihadi

et al. 2018

Bioma

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Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

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“…To the best of our knowledge, there is only one study in which onion medium was used for selective isolation of onion-pathogenic and onion-associated bacteria (Zaid et al 2012). Although these authors used onion extract as the main source of nutrients for their medium, they autoclaved it for 35 min at 120 °C twice.…”

Section: Growth In Culture Medium Prepared With Onion Juice Extractmentioning

confidence: 99%

Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina

Rinland

Gómez

2015

World J Microbiol Biotechnol

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Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.

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OEM—A new medium for rapid isolation of onion-pathogenic and onion-associated bacteria

Cited by 22 publications

References 30 publications

Rahnella spp. are commonly isolated from onion ( Allium cepa ) bulbs and are weakly pathogenic

Rahnella spp. are commonly isolated from onion ( Allium cepa ) bulbs and are weakly pathogenic

Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina

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