Oestrogen and Progesterone Levels in the Blood Plasma of Cows With Normal Parturition or With a Retained Placenta

Agthe, O.; Kolm, H.P.

doi:10.1530/jrf.0.0430163

Cited by 45 publications

(16 citation statements)

References 11 publications

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“…In pregnant cows, the profiles and the levels of both estrogens were almost the same as those described in other reports [2,4,18,19,24]. Furthermore, E1 peaked at the time of parturition and thereafter drastically decreased.…”

Section: Discussionsupporting

confidence: 85%

The Development of a Sensitive Enzymeimmunoassay for the Determination of Estrone and Estradiol-17.BETA. in Bovine Blood Plasma based on the Same Homologous Combination with Antiserum and Steroid-Enzyme Conjugate

Takenouchi

Oshima

Shimada

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The development of a sensitive enzymeimmunoassay (EIA) for the determination of estrone (E1) and estradiol-17β (E2β) in bovine plasma is described. The assay is a homologous double-antibody EIA with E2β 17hemisuccinate (HS) as hapten for the immunoreactive reagent. The antiserum was raised against E2β 17HS bovine serum albumin conjugate in the rabbit, and E2β 17HS-horseradish peroxidase was used as steroid-enzyme conjugate. Each estrogen EIA was distinguished only by using the each working stan dard and sample for the EIA. Bovine plasma E1 and E2β were extracted and purified before EIA. The antiserum was used at 1:1,750,000 dilutions for EIA. Estrone and E2β showed high cross-reactivity with the antiserum (E1: 350.7%, E2β:100%). The sensitivities were <0.03 pg/well for E1 and <0.12 pg/well for E2 β. Recovery rates of E1 and E2β added to bovine blood plasma were 94.5% and 93.9%, respectively. The precision for EIA of estrogens was below 9.7%. The profiles of either estrogen as determined by EIA corresponded closely well with follicle dynamics in the cow during the estrous cycles and with placental function in pregnant animals. In co nclusion, our new EIA can be applied with sufficient sensitivities, recovery and precision for the routine analysis of E1 and E2β concentrations in bovine plasma. KEY WORDS: cattle, enzymeimmunoassay, estrone, estradiol-17β.

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Section: Discussionsupporting

confidence: 85%

The Development of a Sensitive Enzymeimmunoassay for the Determination of Estrone and Estradiol-17.BETA. in Bovine Blood Plasma based on the Same Homologous Combination with Antiserum and Steroid-Enzyme Conjugate

Takenouchi

Oshima

Shimada

et al. 2004

The Journal of Veterinary Medical Science

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“…Chew et al [35] reported lower E2β concentrations before induction of calving but higher E 2 β on the day before calving in cows with RFM than in those without RFM. Agthe and Kolm [23] reported a marked increase in plasma E 2 β concentrations on the day before calving in cows with RFM. In this study, the concentrations of E 2 β from day 10 to day 3 prepartum were significantly lower in the RFM-singleton group compared with the normal-singleton group; however, within days 2 and 1 prepartum, there were no significant differences in the E2β concentrations, but the increments in E 2 β from the concentrations on the preceding day were significantly higher in the RFM-singleton group than the normal-singleton group.…”

Section: Discussionmentioning

confidence: 96%

“…Agthe and Kolm [23] reported a marked increase in the plasma E 2 β concentration within 12 h before parturition in cows with RFM compared with those without RFM. Some authors h a v e r e p o r t e d h i g he r b l o od p r o g e s t e r on e concentrations but lower estrogen concentrations in peripartum cows with RFM [8,22].…”

mentioning

confidence: 96%

Peripartum Changes in Plasma Estrone Sulfate and Estradiol-17.BETA. Profiles Associated With and Without the Retention of Fetal Membranes in Holstein-Friesian Cattle

et al. 2007

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Abstract. The aim of this study was to evaluate the changes in plasma concentrations of estrone sulfate (E1S) and estradiol-17β (E2β) during the peripartum period (from day 10 prepartum to day 1 postpartum) associated with and without retention of fetal membranes (RFM) in Holstein-Friesian cattle (n=42). Plasma samples were analyzed for E1S and E2β by ELISA. All parturitions were spontaneous and normal. Of 38 cattle delivering singletons, 29 had no RFM (singleton-normal group) and nine had RFM for more than 12 h (singleton-RFM group). Four cows gave birth to twins, and each twin had its own fetal membrane (FM). Two twinning cows expelled both FMs normally within 12 h (twin-normal group). In the remaining 2 twinning cows (twin-RFM group), the FM was expelled normally for one twin (first), while the FM of the other (second) was retained. There were no significant differences in the E1S concentrations or their increments from the concentrations on the preceding day between the normal and RFM groups of singleton cows on any peripartum day. The mean plasma E2β concentrations on each day from day 10 to day 3 prepartum were significantly lower (P<0.05) in the singleton-RFM group compared with the singleton-normal group; however, on days 2 and 1 prepartum, the increments in the E2β concentrations from the concentrations on the preceding days were significantly higher (P<0.05) in the singleton-RFM group than in the singleton-normal group. Thus, the plasma E1S concentrations just before parturition may not be associated with RFM. In the cows with RFM, the lower plasma E2β concentrations that were found prior to day 2 prepartum may have been associated with immature placentomes, and the rapid rise in plasma E2β within 1 to 2 days prior to calving may have produced asynchrony of placental and/or fetal maturation in relation to calving, thus resulting in RFM.

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“…Although retained placenta is experimentally induced by the removal of the corpus luteum [111], decreases in serum concentrations of E 2 and progesterone (P 4 ) near parturition are not directly related to the development [1]. The association of fatty liver [122] and hypocalcemia [32] has been reported.…”

Section: Fatty Liver-related Diseasesmentioning

confidence: 99%

Relevance of Apolipoproteins in the Development of Fatty Liver and Fatty Liver-Releted Peripartum Diseases in Dairy Cows.

2002

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ABSTRACT. Most metabolic diseases in dairy cows occur during the peripartum period and are suggested to be derived from fatty liver initially developed during the nonlactating stage. Fatty liver is induced by hepatic uptake of nonesterified fatty acids that are released in excess by adipose tissues attributable to negative energy balance. The fatty accumulation leads to impairment of lipoprotein metabolism in the liver, and the impairment in turn influences other metabolic pathways in extrahepatic tissues such as the steroid hormone production by the corpus luteum. Detailed understanding of the impaired lipoprotein metabolism is crucial for elucidation of the mechanistic bases of the development of fatty liver and fatty liver-related peripartum diseases. This review summarizes results on evaluation of lipoprotein lipid and protein concentrations and enzyme activity in cows with fatty liver and those with ketosis, left displacement of the abomasum, milk fever, downer syndrome and retained placenta. Obtained data strongly suggest that decreases in serum concentrations of apolipoprotein B-100, apolipoprotein A-I and apolipoprotein C-III, a reduction in activity of lecithin:cholesterol acyltransferase and induction of haptoglobin and serum amyloid A are intimately related to the development of fatty liver and fatty liver-related diseases. Moreover, determination of the apolipoprotein concentrations and enzyme activity during the peripartum period is useful for early diagnoses of these diseases. KEY WORDS: apolipoprotein, bovine, estrogen receptor, fatty liver, protein kinase C.J. Vet. Med. Sci. 64(4): 293-307, 2002 PREFACE AND SCOPE OF THIS REVIEW Control of metabolic diseases, including ketosis, left displacement of the abomasum (LDA), milk fever and downer cow syndrome are crucially important for modern dairy husbandry, because their occurrence is intimately related to extensive feeding and management of dairy cows and also because high-yielding cows are particularly susceptible to such diseases. Affected cows, even after recovery from those metabolic diseases, have high incidences of reproductive and infectious diseases. Most metabolic diseases occur during the peripartum period (2 to 4 weeks from parturition) and are suggested to be caused by fatty liver (hepatic lipidosis) initially developed during the nonlactating stage. Fatty liver is a condition in which triglycerides (TG) accumulate in the liver. As a logical consequence, the TG accumulation results in impaired metabolism of lipoproteins, most of which are produced by the liver. Lipoproteins have a role in the transport of lipids between the liver and extrahepatic tissues. Impairment of lipoprotein metabolism is linked to disturbances of other metabolic pathways in extrahepatic tissues, for example, the steroid hormone production in the corpus luteum. Studies on impaired lipoprotein metabolism are therefore prerequisite to elucidate the mechanistic bases of the development of fatty liver and fatty liver-related diseases. Lipoproteins consist of lipids a...

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Oestrogen and Progesterone Levels in the Blood Plasma of Cows With Normal Parturition or With a Retained Placenta

Cited by 45 publications

References 11 publications

The Development of a Sensitive Enzymeimmunoassay for the Determination of Estrone and Estradiol-17.BETA. in Bovine Blood Plasma based on the Same Homologous Combination with Antiserum and Steroid-Enzyme Conjugate

The Development of a Sensitive Enzymeimmunoassay for the Determination of Estrone and Estradiol-17.BETA. in Bovine Blood Plasma based on the Same Homologous Combination with Antiserum and Steroid-Enzyme Conjugate

Peripartum Changes in Plasma Estrone Sulfate and Estradiol-17.BETA. Profiles Associated With and Without the Retention of Fetal Membranes in Holstein-Friesian Cattle

Relevance of Apolipoproteins in the Development of Fatty Liver and Fatty Liver-Releted Peripartum Diseases in Dairy Cows.

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