Oestrogen receptors and protein synthesis in caruncular and intercaruncular endometrium of sheep before implantation

Findlay, J. K.; Clarke, I. J.; Swaney, John B.; Colvin, Noelene; Doughton, B.

doi:10.1530/jrf.0.0640329

Cited by 31 publications

(17 citation statements)

References 34 publications

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“…The affinity for cytosolic PR was similar in all groups (0.65 ± 0.07 nM, n = 20). The binding affinities of both ER and PR were similar to those found in different physiological situations in sheep [6,7,31,32,37]. Nuclear ER Kd (0.47 ± 0.08 nM, n = 8) had an affinity similar to that of the cytosolic receptor.…”

Section: Enzyme Immunoassay (Eia) Of Er Proteinsupporting

confidence: 76%

“…The ER and PR receptor determinations were performed in the cytosolic fractions by a binding assay described previously [7,37]. Briefly, the cytosolic fraction was incubated with 5 to 6 increasing concentrations of [2,4,6,7- [6, H]pregn-4en-3,20-dione 40 Ci . mmol -1 (0.5-30 nM) for 18 h with or without 200-fold molar excess of either unlabeled diethylstilbestrol or unlabeled ORG-2058, respectively.…”

Section: Extraction and Assays Of Steroid Receptorsmentioning

confidence: 99%

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A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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-Regulation of the uterine expression of estrogen and progesterone receptors was studied in 20 three-month-old lambs that were not treated or treated with estradiol-17β. Determinations of receptors were performed by binding assays in the nuclear and cytosolic fractions, receptor mRNAs by solution hybridization, and estrogen receptor protein by an enzyme-immunoassay. Estradiol treatment decreased the receptor binding capacity of both receptors and the levels of immunoreactive estrogen receptor 12 h after injection in the absence of decreased receptor mRNAs, suggesting that the initial decrease is due to degradation of the proteins or that mRNAs are translated into new receptor proteins at a reduced rate. The mRNA levels increased after estradiol treatment suggesting that the replenishment phase consists of synthesis of new receptors rather than recycling of inactivated receptors.estrogen / progesterone / receptor / uterus / lamb Résumé -Action biphasique de l'oestradiol sur l'expression des récepteurs aux oestrogènes et à la progestérone dans l'utérus de l'agnelle. La régulation de l'expression des récepteurs aux oestrogènes et à la progestérone au niveau de l'utérus a été étudiée chez des agnelles immatures âgées de 3 mois (n = 20), traitées et non traitées avec oestradiol-17β. Les déterminations des récepteurs ont été faites par des essais de liaison dans les fractions nucléaires et cytosoliques, les ARNm des récepteurs par la méthode d'hybridation en solution et la protéine du récepteur aux oestrogènes par un essai immuno-enzymatique. Le traitement à l'oestradiol réduit la capacité de liaison des deux récepteurs et les niveaux des récepteurs aux oestrogènes immunoréactifs 12 h après l'injection en absence d'une diminution des ARNm des récepteurs, suggèrent que la réduction initiale est due à la dégradation des Reprod. Nutr. Dev. 40 (2000) 283-293 283

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Section: Enzyme Immunoassay (Eia) Of Er Proteinsupporting

confidence: 76%

Section: Extraction and Assays Of Steroid Receptorsmentioning

confidence: 99%

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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“…Up to now, none of these proteins have been isolated and purified. Segerson (1988) (Findlay et al, 1982). In the same way there were no differences in the secretory activity of the endometrium between pregnant or non-pregnant endometrial explants (Eyestone and French, 1981 ).…”

Section: Introductionmentioning

confidence: 90%

Embryo-uterine interactions during early stages of pregnancy in domestic mammals

Guillomot¹,

Reinaud²,

Charpigny³

et al. 1988

Reprod. Nutr. Dévelop.

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Summary. The We also studied the effects of co-culture with uterine cells on blastocyst DNA and protein synthesis. In no cases we obtained stimulation of blastocyst development during the co-culture period, and DNA or protein synthesis decreased in the presence of uterine cells.In conclusion, the presence of specific uterine proteins has been established in some domestic mammals (pig, rabbit) but not in all of them. Although local modifications of uterine protein synthesis are induced by the embryo or its secretory products, the nature and the role of the proteins which are affected need to be determined by further studies.Introduction.

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“…Endometrial steroid hormone receptor populations change in sheep during the estrous cycle and pregnancy [12,13,23,24]. These changes undoubtedly alter responsiveness of these target tissues to circulating hormones.…”

Section: Introductionmentioning

confidence: 99%

Temporal and Spatial Alterations in Uterine Estrogen Receptor and Progesterone Receptor Gene Expression During the Estrous Cycle and Early Pregnancy in the Ewe1

Spencer

Bazer

1995

Biology of Reproduction

290

208

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Mechanisms regulating responses of the ovine uterus to endocrine and paracrine signals during the estrous cycle and pregnancy are likely to require tissue- and cell-specific regulation of steroid hormone receptor gene expression. To determine effects of day and pregnancy status (cyclic or pregnant) on uterine estrogen receptor (ER) and progesterone receptor (PR) gene expression, ewes were hysterectomized either on Day 1 (Day 0 = estrus/mating), 6, 11, 13, or 15 of the estrous cycle (n = 3/day) or on Day 11, 13, 15, 17, or 25 of early pregnancy (n = 5/day). Steady state levels of ER and PR mRNA were determined in endometrial and myometrial tissues by slot-blot hybridization and ribonuclease protection assays, respectively, using homologous ovine ER and PR cRNA probes. Changes in spatial expression of ER and PR mRNA and protein in uterine tissue sections were determined by in situ hybridization and immunocytochemical analyses. In cyclic ewes, steady state levels of endometrial ER mRNA were highest on Day 1, declined between Days 1 and 6, and increased between Days 11 and 15. However in pregnant ewes, endometrial ER mRNA levels decreased between Days 11 and 15 and increased slightly between Days 15 and 25. In cyclic ewes, levels of myometrial ER mRNA were highest on Day 1, decreased to Day 6, and remained low thereafter. In cyclic ewes, endometrial PR mRNA levels were highest on Day 1, decreased between Days 1 and 11, and then increased between Days 13 and 15. In cyclic ewes, myometrial PR mRNA levels were highest on Day 1 and declined thereafter. Endometrial PR mRNA levels were not different between cyclic and pregnant ewes on Days 11, 13, and 15. In pregnant ewes, PR mRNA levels were low on Day 11, increased between Days 11 and 17, and decreased between Days 17 and 25. In pregnant ewes, myometrial PR mRNA levels were low and did not change between Days 11 and 25. In situ hybridization and immunocytochemical analyses revealed distinct tissue- and cell type-specific alterations in uterine ER and PR mRNA and protein expression during the estrous cycle and early pregnancy that generally paralleled overall changes in steady state levels of ER and PR mRNAs. In the endometrium, the most striking observation was that PR mRNA and protein expression disappeared from the luminal and shallow glandular epithelium between Days 6 and 13 of the estrous cycle, whereas ER mRNA and protein expression was low on Days 6 and 11 and increased between Days 11 and 15 in the luminal and shallow glandular epithelium. During early pregnancy, expression of ER and PR mRNAs, as well as ER and PR protein, was very low or absent in the luminal and shallow glandular epithelium between Days 13 and 25 of pregnancy. Moreover, ER and PR mRNA and protein were consistently present at low levels in the stroma and deep glandular epithelium in both cyclic (Days 11-15) and pregnant (Days 11-25) ewes. Collectively, results suggest that uterine ER and PR gene expression is regulated in a tissue- and cell type-specific manner during the estrous cycle and early...

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Oestrogen receptors and protein synthesis in caruncular and intercaruncular endometrium of sheep before implantation

Abstract: Summary. The rate of protein synthesis was higher in caruncular (P < 0\m=.\05) and intercaruncular endometrium (P < 0\m=.

Cited by 31 publications

References 34 publications

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

Embryo-uterine interactions during early stages of pregnancy in domestic mammals

Temporal and Spatial Alterations in Uterine Estrogen Receptor and Progesterone Receptor Gene Expression During the Estrous Cycle and Early Pregnancy in the Ewe1

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