Oestrogenic activity of parabens in MCF7 human breast cancer cells

Byford, J. R.; Shaw, L. E.; Drew, Michael G. B.; Pope, G. S.; Sauer, Maurice J.; Darbre, Philippa D.

doi:10.1016/s0960-0760(01)00174-1

Cited by 333 publications

(273 citation statements)

References 39 publications

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“…Concern in regard to the safety of parabens has been raised since the first report of the accumulation of MeP in human breast tumors (Darbre et al, 2004). Although controversy still surrounds the carcinogenic potential of parabens (Darbre et al, 2004;Golden et al, 2005), these compounds elicit weak estrogenic effects in animal studies (Byford et al, 2002;Darbre and Harvey, 2008;Lemini et al, 2003;Miller et al, 2001;Okubo et al, 2001;Pugazhendhi et al, 2005;Witorsch and Thomas, 2010). The estrogenic activity of parabens increases with the length of the alkyl chain; MeP, EtP, PrP, and BuP exhibit estrogenic potencies that are 2 500 000-, 150 000-, 30 000-, and 10 000-fold, respectively, less than that of 17β-estradiol (Routledge et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of parabens in foodstuffs from China and its implications for human dietary exposure

Liao

Chen

Kannan

2013

Environment International

173

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Parabens are alkyl esters of p-hydroxybenzoic acid and are used as antimicrobial preservatives in a range of consumer products, including cosmetics, pharmaceuticals, and foodstuffs. Despite their widespread use, prior to this study, paraben concentrations in foodstuffs from China and human dietary exposure to these chemicals have been unknown. In this study, concentrations of six parabens were determined in 13 categories of food samples (n = 282), including cereals and cereal products, meat, fish and seafood, eggs, dairy products, bean products, fruits, vegetables, cookies, beverages, cooking oils, condiments, and others, collected from nine cities in China. Almost all (detection rate: 99%) food samples contained at least one of the parabens analyzed, and the total concentrations (ΣParabens; sum of six parabens) ranged from below limit of quantification (LOQ) to 2530 ng/g fresh weight, with an overall mean value of 39.3 ng/g. Methyl paraben (MeP), ethyl paraben (EtP), and propyl paraben (PrP) were the major paraben analogs found in foodstuffs, and these compounds accounted for 59%, 24%, and 10%, respectively, of ΣParaben concentrations. Although the mean concentrations of ΣParabens varied among different categories of food items (from 0.839 ng/g in beverages to 100 ng/g in vegetables), the concentrations were not statistically significant among the 13 food categories, including canned foodstuffs. Estimated daily intake (EDI) of parabens was based on the measured concentrations in foods and the corresponding daily food ingestion rates. The mean and 95th percentile values for EDI were 1010 and 3040 ng/kg body weight (bw)/day for adult men and 1060 and 3170 ng/kg bw/day for adult women, respectively.

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Section: Introductionmentioning

confidence: 99%

Occurrence of parabens in foodstuffs from China and its implications for human dietary exposure

Liao

Chen

Kannan

2013

Environment International

173

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“…Many cosmetic products, including antiperspirants, contain parabens that can be dermally absorbed. Parabens have estrogen-like properties in cell cultures, causing proliferation of estrogen-responsive cells, although they are thousands of times less potent than naturally-occurring estrogen in this regard [164][165][166]. Parabens were found to activate both estrogen receptors, ER-α and ER-β, with similar or stronger effect versus ER-β receptors [165,167,168].…”

Section: Parabensmentioning

confidence: 99%

“…Competitive inhibition of 17β-estradiol binding to estrogen-dependent MCF7 cell ERs could be detected at 1,000,000-fold molar excess of butylparaben (BuP, 86%), propylparaben (PrP, 77%), ethylparaben (EtP, 54%) and methylparaben (MeP, 21%). Molecular modelling has indicated the mode by which paraben molecules can bind into the ligand binding pocket of the crystal structure of the ligand binding domain of ER-α in place of 17β-estradiol, and it has been shown that two paraben molecules can bind simultaneously in a mode, in which their phenolic hydroxyl groups bind similarly to those of the hexestrol molecule [164].…”

Section: Parabensmentioning

confidence: 99%

Impact of Environmental Contaminants on Breast Cancer / Wpływ Zanieczyszczenia Środowiska Na Raka Piersi

Kráľová

Jampílek

2015

Ecological Chemistry and Engineering S

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Breast cancer is the most frequent cancer in women. It is believed that among the causes of breast cancer, hereditary factors account for only 5-10% of risk and the environmental exposures to environmental contaminants account for an additional 30-50% of risk. This paper summarizes findings related to the risk of breast cancer due to exposure to following environmental contaminants: polycyclic aromatic hydrocarbons, polychlorinated biphenyls and dioxins, organochlorine pesticides, organophosphorous pesticides, bisphenol A, phthalates, parabens, organic solvents, atmospheric pollutants, alkylphenols, metals, ionizing radiation, electromagnetic field and light pollution. Results obtained in in vitro experiments with breast cancer cell lines and in vivo with model rodents as well as in population based case-control studies are presented and the mode of action of individual environmental contaminants on mammary gland is discussed. Attention is also devoted to the effects of the timing of exposure to environmental contaminants (mainly exposition during development of the mammary gland) on breast cancer risk. Outcomes of professional exposure to some environmental contaminants on breast cancer risk are analysed as well.

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“…Parabens can mimic estrogen action stimulating the growth of MCF7 cells, which are related to the development of breast cancer. 4,5 Parabens at trace levels have been determined in several biological matrices, such as urine, 2,6 breast milk, [7][8][9][10][11] plasma, 12 serum, 13 amniotic fluid, 14 and placental tissue. 15,16 As known, lipophilic substances are mainly excreted in breast milk, and consequently, that breast milk is the major way through which breastfed infants are exposed to environmental chemicals.…”

Section: Introductionmentioning

confidence: 99%

Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

Grecco¹,

Souza²,

Acquaro³

et al. 2018

J. Braz. Chem. Soc.

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A reliable method was developed and validated based on dispersive liquid-liquid microextraction (DLLME) and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) to determine simultaneously methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), and butylparaben (BuPB) in breast milk samples. A central composite design 2 4 with four independent variables and five levels of variance was used to optimize the DLLME conditions. The DLLME/UHPLC-MS/MS method was linear from 5 (lower limit of quantification, LLOQ) to 100 ng mL -1 (upper limit of quantification, ULOQ). The inter-assay and intra-assay precision values presented coefficients of variation (CVs) lower than 15%, and the relative standard error of the inter-and intra-assay accuracy values was lower than 10.4%. The DLLME/UHPLC-MS/MS method was successfully applied to determine parabens in human milk samples obtained from sixteen volunteer lactating mothers.

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Oestrogenic activity of parabens in MCF7 human breast cancer cells

Cited by 333 publications

References 39 publications

Occurrence of parabens in foodstuffs from China and its implications for human dietary exposure

Occurrence of parabens in foodstuffs from China and its implications for human dietary exposure

Impact of Environmental Contaminants on Breast Cancer / Wpływ Zanieczyszczenia Środowiska Na Raka Piersi

Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

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