Of coiled bodies, gems, and salmon

Matera, A. Gregory

doi:10.1002/(sici)1097-4644(19980801)70:2<181::aid-jcb4>3.0.co;2-k

Cited by 63 publications

(31 citation statements)

References 78 publications

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“…A more basic question is whether or not CBs require a tether in the first place. In other words, do CBs require specific DNA or RNA sequences in order to nucleate, or can they form independently (Matera, 1998)? Previous studies can shed some light here.…”

Section: Assembly Of Nuclear Bodiesmentioning

confidence: 99%

Nuclear Bodies: Random Aggregates of Sticky Proteins or Crucibles of Macromolecular Assembly?

et al. 2009

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The principles of self-assembly and self-organization are major tenets of molecular and cellular biology. Governed by these principles, the eukaryotic nucleus is composed of numerous subdomains and compartments, collectively described as nuclear bodies. Emerging evidence reveals that associations within and between various nuclear bodies and genomic loci are dynamic and can change in response to cellular signals. This review will discuss recent progress in our understanding of how nuclear body components come together, what happens when they form, and what benefit these subcellular structures may provide to the tissues or organisms in which they are found.

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Section: Assembly Of Nuclear Bodiesmentioning

confidence: 99%

Nuclear Bodies: Random Aggregates of Sticky Proteins or Crucibles of Macromolecular Assembly?

et al. 2009

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“…Coilin, a major structural scaffolding protein necessary for CB formation, composition and activity, is predominantly found in CBs, but is also distributed throughout the nucleoplasm [2], [3], [4]. Coilin orthologues have been identified by sequence comparison and cloning in Xenopus [5], mouse [6], Arabidopsis [7] and Drosophila [8] and have been shown to localize in prominent nuclear bodies in these organisms.…”

Section: Introductionmentioning

confidence: 99%

Plant Coilin: Structural Characteristics and RNA-Binding Properties

et al. 2013

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Cajal bodies (CBs) are dynamic subnuclear compartments involved in the biogenesis of ribonucleoproteins. Coilin is a major structural scaffolding protein necessary for CB formation, composition and activity. The predicted secondary structure of Arabidopsis thaliana coilin (Atcoilin) suggests that the protein is composed of three main domains. Analysis of the physical properties of deletion mutants indicates that Atcoilin might consist of an N-terminal globular domain, a central highly disordered domain and a C-terminal domain containing a presumable Tudor-like structure adjacent to a disordered C terminus. Despite the low homology in amino acid sequences, a similar type of domain organization is likely shared by human and animal coilin proteins and coilin-like proteins of various plant species. Atcoilin is able to bind RNA effectively and in a non-specific manner. This activity is provided by three RNA-binding sites: two sets of basic amino acids in the N-terminal domain and one set in the central domain. Interaction with RNA induces the multimerization of the Atcoilin molecule, a consequence of the structural alterations in the N-terminal domain. The interaction with RNA and subsequent multimerization may facilitate coilin’s function as a scaffolding protein. A model of the N-terminal domain is also proposed.

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“…In metabolically active cells an intensification of the RNA synthesis and processing is usually observed and leads to higher levels of transcriptional/splicing machinery elements in these cells. The model of snRNP biogenesis, proposed by Matera [12], assumes that CBs play an essential role in both snRNAs transcription and the final steps of snRNPs maturation. It has been shown that several snRNA encoding genes, including U1 and U2 snRNAs, are spatially adjacent to the CBs periphery [46, 47].…”

Section: Discussionmentioning

confidence: 99%

“…However, because of the fact that CBs are especially rich in splicing machinery elements, including snRNPs and Sm proteins, it is suggested that they are an important element of the cellular splicing system. According to the hypothesis of Matera [12], transcription of snRNA encoding genes occurs at the periphery of CBs and newly formed snRNAs are targeted to the cytoplasm, where the Sm core domain is assembled and TMG cap is formed [3, 13]. Finally, snRNPs return into the nucleus and localize in Cajal bodies, where the methylation and pseudourydilation of these molecules take place [14].…”

Section: Introductionmentioning

confidence: 99%

snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

Zienkiewicz

Bednarska

2009

International Journal of Cell Biology

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The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells of Hyacinthus orientalis L.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identified as Cajal bodies. In all cases, a strong accumulation of snRNP-indicating molecules including TMG cap, Sm proteins and U2 snRNA, was observed in the examined nuclear bodies. In contrast to their number the size of the identified structures did not change significantly during pollen development. In the microspore and the vegetative cell of pollen grains CBs were more numerous than in the generative cell. At later stages of pollen development, a drastic decrease in CB number was observed and, just before anthesis, a complete lack of these structures was indicated in both pollen nuclei. On the basis of these results, as well as our previous studies, we postulate a strong relationship between Cajal body numbers and the levels of RNA synthesis and splicing machinery elements in microspore and developing pollen cells.

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Of coiled bodies, gems, and salmon

Cited by 63 publications

References 78 publications

Nuclear Bodies: Random Aggregates of Sticky Proteins or Crucibles of Macromolecular Assembly?

Nuclear Bodies: Random Aggregates of Sticky Proteins or Crucibles of Macromolecular Assembly?

Plant Coilin: Structural Characteristics and RNA-Binding Properties

snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

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