Atmospheric profiling is a requirement for controlling wide-field Adaptive Optics (AO) instruments, analyzing the AO performance with respect to the observing conditions and predicting the Point Spread Function (PSF) spatial variations. We present PEPITO, a new concept for profiling atmospheric turbulence from post facto tip-tilt (TT) corrected short-exposure images. PEPITO utilizes the anisokinetism effect in the images between several stars separated from a reference star, and then produces the profile estimation using a model-fitting methodology, by fitting to the long exposure TT-corrected PSF. PEPITO has a high sensitivity to both C 2 n (h) and L 0 (h) by relying on the full telescope aperture and a large field of view. It then obtains a high vertical resolution (1 m-400 m) configurable by the camera pixel scale, taking advantage of fast statistical convergence (of order of tens of seconds). With only a short exposure-capable large format detector and a numerical complexity independent of the telescope diameter, PEPITO perfectly suits accurate profiling for night optical turbulence site characterization or adaptive optics instruments operations. We demonstrate, in simulation, that the C 2 n (h) and L 0 (h) can be estimated to better than 1% accuracy, from fitted PSFs of magnitude V=11 on a D=0.5 m telescope with a 10 arcmin field of view.