Off-chip passivated-electrode, insulator-based dielectrophoresis (OπDEP)

Zellner, Phillip; Shake, Tayler; Sahari, Ali; Behkam, Bahareh; Agah, Masoud

doi:10.1007/s00216-013-7123-7

Cited by 29 publications

(30 citation statements)

References 30 publications

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“…It is an advancement of our previously reported 2D iDEP and an alternative to our 3D iDEP device. 17,20 As demonstrated in our results, 3D structures generate stronger DEP forces in comparison to 2D microstructures in iDEP devices, and the passivated electrodes are reusable. The stronger electric field gradient generated is further supported by the theory presented earlier in Eq.…”

Section: Discussionsupporting

confidence: 70%

“…This is two orders of magnitude greater than the peak gradients using the exact same input signal in our previous iDEP devices which only varied in two dimensions. 20 To obtain an estimation of the Clausius-Massotti factor (f CM ), the electrical parameters of S. aureus bacteria and the surrounding DI water medium shown in Table I were used. 12 Using Eqs.…”

Section: A Numerical Modelingmentioning

confidence: 99%

“…This 3D pDEP is comparable to our previously reported passivated-electrode insulatorbased dielectrophoresis (OpDEP) device. 20 OpDEP combines the benefits of a high throughput, low cost 2D iDEP, and the enhanced sensitivity of eDEP with electrodes located in the vicinity of the 2D insulating microstructures. A reusable set of electrodes are activated and the signal capacitively coupled through a thin glass slide into the microfluidic channel.…”

Section: Introductionmentioning

confidence: 99%

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Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D pDEP) is presented. This technology combines the benefits of electrodebased DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D pDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 lm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D pDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 ll/h and 70% at flow rates as high as 750 ll/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed at applied voltages as low as 50 Vpp. We demonstrate selective trapping of live and dead bacteria at frequencies ranging from 30 to 60 kHz at 400 Vpp with over 90% of the live bacteria trapped while most of the dead bacteria escape. V C 2015 AIP Publishing LLC.

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Section: Discussionsupporting

confidence: 70%

Section: A Numerical Modelingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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“…This translates to limited frequency ranges due to equipment restrictions and large electronics. Further limitations of the platform are discussed in Zellner et al [22]. In addition, as this study evaluated frequencies at 100 kHz intervals, the DEP profiles generated are only discrete values.…”

Section: Discussionmentioning

confidence: 99%

Breast cancer cell obatoclax response characterization using passivated‐electrode insulator‐based dielectrophoresis

et al. 2017

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Inherent electrical properties of cells can be beneficial to characterize different cell lines and their response to experimental drugs. This paper presents a novel method to characterize the response of breast cancer cells to drug stimuli through use of off-chip passivated-electrode insulator-based dielectrophoresis (OπDEP) and the application of AC electric fields. This work is the first to demonstrate the ability of OπDEP to differentiate between two closely related breast cancer cell lines, LCC1 and LCC9 while assessing their drug sensitivity to an experimental anti-cancer agent, Obatoclax. Although both cell lines are derivatives of estrogen-responsive MCF-7 breast cancer cells, growth of LCC1 is estrogen independent and anti-estrogen responsive, while LCC9 is both estrogen-independent and anti-estrogen resistant. Under the same operating conditions, LCC1 and LCC9 had different DEP profiles. LCC1 cells had a trapping onset (crossover) frequency of 700 kHz and trapping efficiencies between 30–40%, while LCC9 cells had a lower crossover frequency (100 kHz) and showed higher trapping efficiencies of 40–60%. When exposed to the Obatoclax, both cell lines exhibited dose-dependent shifts in DEP crossover frequency and trapping efficiency. Here, DEP results supplemented with cell morphology and proliferation assays help us to understand the response of these breast cancer cells to Obatoclax.

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“…21 Zellner et al fabricated contactless DEP device to separate cells and polystyrene beads using a glass substrate as the barrier. 22 The contactless DEP devices do not require metal electrodes, or the metal electrodes can be reused because they do not need to be bonded to PDMS in the device. Unfortunately, the contactless AC DEP devices require high voltage with high frequency in order to cross the insulating barrier and generate strong enough electric field gradient.…”

Section: Introductionmentioning

confidence: 99%

Continuous On-Chip Cell Separation Based on Conductivity-Induced Dielectrophoresis with 3D Self-Assembled Ionic Liquid Electrodes

et al. 2016

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Dielectrophoresis (DEP) has been widely explored to separate cells for various applications. However, existing DEP devices are limited by the high cost associated with the use of noble metal electrodes, the need of high-voltage electric field, and/or discontinuous separation (particularly for devices without metal electrodes). We developed a DEP device with liquid electrodes, which can be used to continuously separate different types of cells or particles based on positive DEP. The device is made of polydimethylsiloxane (PDMS) and ionic liquid is used to form the liquid electrodes, which has the advantages of low cost and easy fabrication. Moreover, the conductivity gradient is utilized to achieve the DEP-based on-chip cell separation. The device was used to separate polystyrene microbeads and PC-3 human prostate cancer cells with 94.7 and 1.2% of the cells and microbeads being deflected, respectively. This device is also capable of separating live and dead PC-3 cancer cells with 89.8 and 13.2% of the live and dead cells being deflected, respectively. Moreover, MDA-MB-231 human breast cancer cells could be separated from human adipose-derived stem cells (ADSCs) using this device with high purity (81.8 and 82.5% for the ADSC and MDA-MB-231 cells, respectively). Our data suggest the great potential of cell separation based on conductivity-induced DEP using affordable microfluidic devices with easy operation.

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Off-chip passivated-electrode, insulator-based dielectrophoresis (OπDEP)

Cited by 29 publications

References 30 publications

Three dimensional passivated-electrode insulator-based dielectrophoresis

Three dimensional passivated-electrode insulator-based dielectrophoresis

Breast cancer cell obatoclax response characterization using passivated‐electrode insulator‐based dielectrophoresis

Continuous On-Chip Cell Separation Based on Conductivity-Induced Dielectrophoresis with 3D Self-Assembled Ionic Liquid Electrodes

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