In an exploration of biophilic cities, this study examines the integration of nature into urban environments, emphasizing its critical importance to human well-being. Biophilic cities are characterized by abundant green and blue spaces, facilitating human interaction within natural settings. These cities prioritize pedestrian spaces, cultural and historical sites linked to nature, and diverse usage ensuring habitat conservation. To ascertain the key factors enabling effective and sustainable incorporation of nature into urban spaces, two primary components were identified. Firstly, the block-level nature integration factor was investigated, represented by the Meso Index. This index is calculated by the green area's ratio within a designated block. Secondly, the citywide nature integration factor, which emphasizes the interconnectedness of green spaces throughout the city, was examined. This factor promotes direct interaction with nature across various urban functions, including housing, commerce, education, and recreation. Both the lawn afforestation index and the linear garden index serve as measures for this integration. Direct and indirect indicators affected by the establishment of biophilic cities were then applied to Kufa City, Iraq. Employing GIS for map production and analysis alongside mathematical models, indicators were analyzed through field surveys, observation, and feedback forms. Results revealed the innate human predisposition towards nature, highlighting its significance in daily life. Adverse effects on human life and behavior were observed in areas devoid of greenery. Kufa City exhibited potential for biophilic transformation, contingent upon accurate indicator application and improved green space planning.