Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. and Candida mattranensis sp. nov., three methylotrophic yeast species from phylloplane in Thailand

Abstract

Five strains (LN12, LN14(T), LN15(T), LN16 and LN17(T)) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14(T) and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14(T) (= BCC 42666(T) = NBRC 107780(T) = CBS 12095(T)). Strain LN15(T) was assigned to be Candida chumphonensis sp. nov. (type strain LN15(T) = BCC 42667(T) = NBRC 107781(T) = CBS 12096(T)). Strain LN17(T) represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17(T) = BCC 42668(T) = NBRC 107782(T) = CBS 12097(T)).

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

“…Moreover, the microbial communities of the phylloplane are dependent of several factors like environmental factors, leaf age, particular growing conditions of the plant, and on the type and density of competing microorganisms [3,6,7]. The phylloplane of plants is colonized by several yeasts of the phyla Ascomycota and Basidiomycota [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22].…”

“…The phylloplane yeast community has been studied by both culture-dependent and culture-independent approaches. Recent studies addressed the topic of yeast diversity on the phylloplane of economically relevant crops and both culture-dependent and culture-independent approaches have been employed [13][14][15][16][17][18][19][20][21][22]. As observed for other microbes, the species composition of the yeast communities is not identical when the two approaches are compared or when culture-dependent approaches employ different yeast isolation techniques [16][17][18][19][20][21]23].…”

Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves

“…In addition to the 31 known species of Ogataea listed by Kurtzman (2011), nine species belonging to this clade were described recently (Čadež et al , 2013). Most species of Ogataea are associated with rotting wood, the external surfaces of leaves or insect frass, or as minor components of yeast communities on bodies of water and olive oil (Kurtzman 2011; Koowadjanakul et al , 2011; Limtong et al , 2013; Čadež et al , 2013).…”

“…Several species of the Ogataea clade have been described as associated with the phylloplane, such as O . allantospora , O. kanchanaburienis , O. nitratoaversa , O. phyllophila , O. saltuana and O. wangdongensis (Koowadjanakul et al ., 2011; Péter et al , 2011; Čadež et al , 2013; Limtong et al , 2013). It is likely that these methylotrophic yeasts utilize methanol and other leaching carbon sources from the leaves, as suggested by Péter et al (2007).…”

Candida phyllophila sp. nov. and Candida vitiphila sp. nov., two novel yeast species from grape phylloplane in Thailand

“…Basidiomycetous species belonging to the genera Cryptococcus, Rho dotorula, Sporobolomyces and Trichosporon have been found to be the most common phylloplane yeasts (de Azeredo et al, 1998;Glushakova and Chernov, 2010;Slavikova et al, 2009); however, in recent years, more ascomycete yeast species have been detected on the phylloplane, such as Debaryomyces han senii, Hanseniaspora uvarum, Kazachstania barnetii, Metschnikowia pulcherrima, Metschnikowia sacchari cola, Metschnikowia lopburiensis, Pichia membranifa ciens, Saccharomyces cerevisiae and various species of Candida including C. aechmeae, C. chumphonensis, C. maesa, C. mattranensis, C. stauntonica and C. vries eae (Chang et al, 2012;Glushakova and Chernov, 2010;Kaewwichian et al, 2012;Koowadjanakul et al, 2011;Landell et al, 2010;Slavikova et al, 2009).…”