Oil-in-Water fL Droplets by Interfacial Spontaneous Fragmentation and Their Electrical Characterization

Arrabito, Giuseppe; Errico, Vito; Ninno, Adele De; Cavaleri, Felicia; Ferrara, Vittorio; Pignataro, Bruno; Caselli, Federica

doi:10.1021/acs.langmuir.8b04316

Cited by 8 publications

(8 citation statements)

References 72 publications

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“…In turn, this can lead to the nonspecific adsorption of biomolecules at the oil–water interface which can potentially affect the biological activity . Several reports have shown the possibility to produce fL‐droplets following printing approaches as different as pin‐printing, ultrafine nozzles printing, hydrodynamic droplet dispensing under electrical field guiding, droplets production within liquid environments, satellite droplets printing, liquid meniscus break‐up in a double‐orifice system, or spontaneous interfacial droplet fragmentation . However, these printing methods suffer from complex experimental set‐ups and require specialized hardware, making difficult their rapid implementation in chemistry laboratories.…”

Section: Introductionmentioning

confidence: 99%

Printing Life‐Inspired Subcellular Scale Compartments with Autonomous Molecularly Crowded Confinement

et al. 2019

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A simple, rapid, and highly controlled platform to prepare life‐inspired subcellular scale compartments by inkjet printing has been developed. These compartments consist of fL‐scale aqueous droplets (few µm in diameter) incorporating biologically relevant molecular entities with programmed composition and concentration. These droplets are ink‐jetted in nL mineral oil drop arrays allowing for lab‐on‐chip studies by fluorescence microscopy and fluorescence life time imaging. Once formed, fL‐droplets are stable for several hours, thus giving the possibility of readily analyze molecular reactions and their kinetics and to verify molecular behavior and intermolecular interactions. Here, this platform is exploited to unravel the behavior of different molecular probes and biomolecular systems (DNA hairpins, enzymatic cascades, protein‐ligand couples) within the compartments. The fL‐scale size induces the formation of molecularly crowded confined shell structures (hundreds of nanometers in thickness) at the droplet surface, allowing discovery of specific features (e.g., heterogeneity, responsivity to molecular triggers) that are mediated by the intermolecular interactions in these peculiar environments. The presented results indicate the possibility of using this platform for designing nature‐inspired confined reactors allowing for a deepened understanding of molecular confinement effects in living subcellular compartments.

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Section: Introductionmentioning

confidence: 99%

Printing Life‐Inspired Subcellular Scale Compartments with Autonomous Molecularly Crowded Confinement

et al. 2019

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“…Furthermore, they successfully loaded them with DOX, permitting a pH-responsive delivery to tumor cells (B16F10 and 4T1) at similar levels compared to free administrated DOX [155] (Figure 4H). As in the case of microfluidic systems, in 2018, the inkjet printing technologies [156] were employed for emulsion preparations, [157][158][159] and implemented for vesicle assembly, with high reproducibility and size control.…”

Section: Microfluidics and Printing Approachesmentioning

confidence: 99%

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Leggio

Arrabito

Ferrara

et al. 2020

Adv Healthcare Materials

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Naturally occurring extracellular vesicles and artificially made vesicles represent important tools in nanomedicine for the efficient delivery of biomolecules and drugs. Since its first appearance in the literature 50 years ago, the research on vesicles is progressing at a fast pace, with the main goal of developing carriers able to protect cargoes from degradation, as well as to deliver them in a time‐ and space‐controlled fashion. While natural occurring vesicles have the advantage of being fully compatible with their host, artificial vesicles can be easily synthetized and functionalized according to the target to reach. Research is striving to merge the advantages of natural and artificial vesicles, in order to provide a new generation of highly performing vesicles, which would improve the therapeutic index of transported molecules. This progress report summarizes current manufacturing techniques used to produce both natural and artificial vesicles, exploring the promises and pitfalls of the different production processes. Finally, pros and cons of natural versus artificial vesicles are discussed and compared, with special regard toward the current applications of both kinds of vesicles in the healthcare field.

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“…Our group has recently demonstrated the possibility to produce fL-scale oil-in-water droplets by piezoelectric IJP after spontaneous interfacial droplet fragmentation. [216] In particular, pL-scale fluorinated oil drops were printed onto a surfactant-laden water surface at moderately high We number (~ 10 1 ), then they spread, and fragment at the water/air interface.…”

Section: Primitive Autonomous Objectsmentioning

confidence: 99%

Artificial Biosystems by Printing Biology

Arrabito

Ferrara

Bonasera

et al. 2020

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The continuous progress of printing technologies over the past 20 years has fueled the development of a wide plethora of applications in materials sciences, flexible electronics and biotechnologies. More recently, printing methodologies have started up to explore the world of Artificial Biology, offering new paradigms in the direct assembly of Artificial Biosystems (small condensates, compartments, networks, tissues and organs) by mimicking the result of the evolution of living systems and also by redesigning natural biological systems, taking inspiration from them. This recent progress is reported in terms of a new field here defined as Printing Biology, resulting from the intersection between the field of printing and the bottom up Synthetic Biology. Printing Biology explores new approaches for the reconfigurable assembly of designed lifelike or life-inspired structures. This review presents this emerging field, highlighting its main features, i.e. printing methodologies (from 2D to 3D), molecular ink properties, deposition mechanisms, and finally the applications and future challenges. Printing Biology is expected to show a growing impact on the development of biotechnology and lifeinspired fabrication. Printing Biology employs different technologies dispensing molecular inks with tunable composition (molecules, polymers, biomolecules) and drop sizes (from nano-up to macroscale) onto solids or into liquids to develop lifelike or life-inspired artificial biosystems (from small condensates, to compartments up to networks, tissues and organs). This work reviews the extraordinary potential of this emerging research field.

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Oil-in-Water fL Droplets by Interfacial Spontaneous Fragmentation and Their Electrical Characterization

Cited by 8 publications

References 72 publications

Printing Life‐Inspired Subcellular Scale Compartments with Autonomous Molecularly Crowded Confinement

Printing Life‐Inspired Subcellular Scale Compartments with Autonomous Molecularly Crowded Confinement

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Artificial Biosystems by Printing Biology

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