Oleaginous yeasts: Biodiversity and cultivation

Poontawee, Rujiralai; Lorliam, Wanlapa; Polburee, Pirapan; Limtong, Savitree

doi:10.1016/j.fbr.2022.11.003

Cited by 23 publications

(18 citation statements)

References 208 publications

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“…The yeast was grown on yeast peptone dextrose (YPD) medium (20 g/L glucose, 10 g/L peptone, 10 g/L yeast extract, and 15 g/L agar) [4]. The inoculum for fermentation was prepared using 10 mL of minimal medium components: 70 g/L glucose, 0.4 g/L KH 2 PO 4 , 0.55 g/L (NH 4 ) 2 SO 4 , 2.0 g/L MgSO 4 •7H 2 O, and 0.75 g/L yeast extract [2]. It was then incubated at 30 • C for two days on rotary shaker at 150 rpm for 48 h [4].…”

Section: Microorganism and Culture Conditionsmentioning

confidence: 99%

“…The surge in energy demand in recent times has led to increasing interest in novel alternative feedstocks, such as bio-lipids, which are generated by microorganisms and have high energy density and eco-friendly traits [1]. Microorganisms such as bacteria, algae, yeast, and fungi are capable of accumulating intracellular lipids, with yields exceeding 20% of their dry weight [2]. In addition, microbial lipids have drawn much attention for many applications, including industrial biofuel and oleochemical applications.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Lipid and Carotenoid Production via Rhodotorula paludigena CM33 Using Crude Glycerol as the Main Substrate: Pilot-Scale Experiments

Sriphuttha,

Boontawan,

Boonyanan

et al. 2023

IJMS

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Rhodotorula paludigena CM33 is an oleaginous yeast that has been demonstrated to accumulate substantial quantities of intracellular lipids and carotenoids. In this study, crude glycerol, a by-product of biodiesel production, was used as a carbon source to enhance the accumulation of lipids and carotenoids in the cells. The culture conditions were first optimized using response surface methodology, which revealed that the carotenoid concentration and lipid content improved when the concentration of crude glycerol was 40 g/L. Different fermentation conditions were also investigated: batch, repeated-batch, and fed-batch conditions in a 500 L fermenter. For fed-batch fermentation, the maximum concentrations of biomass, lipids, and carotenoids obtained were 46.32 g/L, 37.65%, and 713.80 mg/L, respectively. A chemical-free carotenoid extraction method was also optimized using high-pressure homogenization and a microfluidizer device. The carotenoids were found to be mostly beta-carotene, which was confirmed by HPLC (high pressure liquid chromatography), LC-MS (liquid chromatography-mass spectrometry), and NMR (nuclear magnetic resonance). The results of this study indicate that crude glycerol can be used as a substrate to produce carotenoids, resulting in enhanced value of this biodiesel by-product.

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Section: Microorganism and Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Lipid and Carotenoid Production via Rhodotorula paludigena CM33 Using Crude Glycerol as the Main Substrate: Pilot-Scale Experiments

Sriphuttha,

Boontawan,

Boonyanan

et al. 2023

IJMS

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“…Different types of feedstocks have been used to grow oleaginous yeast. However, glucose remains the most used substrate to grow these microorganisms despite being an expensive carbon source, which increases the costs of the overall process [14]. Therefore, efforts must be made to find low-cost substrates for yeast lipid production and improve the conversion efficiently of these substrates into intracellular lipids.…”

Section: Low-cost Feedstock For Oleaginous Yeast Productionmentioning

confidence: 99%

Oleaginous Yeast Biorefinery: Feedstocks, Processes, Techniques, Bioproducts

Lopes da Silva,

Fontes,

Reis

et al. 2023

Fermentation

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The world climate crisis has triggered the search for renewable energy sources. Oleaginous yeasts are a potential renewable source of biofuels. However, the yeast-derived biofuels cost is still non-competitive with the fossil fuel prices. To improve the sustainability of yeast-derived biofuels, it is necessary to valorize all yeast biomass fractions, an approach based on the biorefinery concept. This review describes the present situation of the oleaginous yeast biorefinery research, emphasizing the feedstock, processes and techniques involved in this concept, as well as on potential bioproducts that can be obtained from oleaginous yeast biomass.

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“…This is because OY has advantages over other OMs, namely the characteristics of single-celled yeasts with high growth ability and coupled with high cellular lipid content. Yeast * Corresponding author: g.l.utama@unpad.ac.id cultivation is also considered easier to improve because it can be processed in a fermenter and does not require light for the growth process (4). OY can also take advantage of cheap or low-cost substrates and even use waste like WCO.…”

Section: Introductionmentioning

confidence: 99%

The potential of Yarrowia lipolytica in converting bioenergy resources: a preliminary review

Puspita Sari,

Lara Utama

2024

E3S Web Conf.

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Yarrowia lipolytica, a yeast species capable of producing oil or oily fatty acids, has the ability to utilize multiple carbon sources, including glycerol, acetic acid, and glucose, allows for the use of inexpensive carbon sources. Waste cooking oil can be utilized as an alternative carbon source while also there is potential in increasing the oil yield due to the presence of glycerol compounds. The study aims to explore the potential of Yarrowia lipolytica in producing lipid based bioenergy from by-product such waste cooking oils. One of the greatest challenges that will affect life is our continued reliance on fossil fuels, which are still derived from petroleum and fossils. Fuel is not only the primary source of energy that has a significant impact on every aspect, but its sustainability remains the primary concern as we search for alternative solutions that can circumvent these issues. Using yeast lipids, specifically Yarrowia lipolytica, has not been investigated, in addition to producie biodiesel, this yeast can use waste cooking oil as a growth medium and produce lipids. The third generation of biodiesel uses microorganism-produced lipids, which is new and worthy of further research to solve the problem of unsustainable and environmentally unfriendly diesel fuel. Yarrowia lipolytica's ability to accumulate lipids, produce wax esters synthase enzymes, and FAEE/FAME still have great potential.

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Oleaginous yeasts: Biodiversity and cultivation

Cited by 23 publications

References 208 publications

Simultaneous Lipid and Carotenoid Production via Rhodotorula paludigena CM33 Using Crude Glycerol as the Main Substrate: Pilot-Scale Experiments

Simultaneous Lipid and Carotenoid Production via Rhodotorula paludigena CM33 Using Crude Glycerol as the Main Substrate: Pilot-Scale Experiments

Oleaginous Yeast Biorefinery: Feedstocks, Processes, Techniques, Bioproducts

The potential of Yarrowia lipolytica in converting bioenergy resources: a preliminary review

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