Oleic acid in Angus and Hanwoo (Korean native cattle) fat reduced the fatty acid synthase activity in rat adipose tissues

Abstract

This study aimed to determine the blood lipid profiles, fatty acid composition, and lipogenic enzyme activities in rat adipose tissues as affected by the Angus beef fat (ABF) and Hanwoo beef fat (HBF) containing high oleic acid (OA) content. We assigned 60 Sprague Dawley rats with a mean bodyweight of 249 ± 3.04 g to three groups (n = 20 each) to receive diets containing 7% coconut oil (CON), 7% ABF, or 7% HBF. The OA content was highest in the HBF (45.23%) followed by ABF (39.51%) and CON (6.10%). The final body weight of the HBF-fed group was significantly increased, probably due to increased feed intake, indicating the palatability of the diet. The HBF and ABF significantly increased high-density lipoprotein cholesterol (HDL-C), decreased triglyceride (TG) and total cholesterol (TC) levels, and also tended to attenuate glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in the bloodstream of the rats compared to CON. As compared to CON, lauric, myristic, and palmitic acids were significantly lower, and those of OA and α-linolenic acid (ALA) were significantly higher in the adipose tissues of HBF and ABF-fed groups. The HBF and ABF also reduced lipogenesis as induced by depleted fatty acid synthase (FAS) activity in rat adipose tissues. Nevertheless, between the two fats, HBF showed high feed intake due to its high palatability but reduced lipogenic enzyme activity, specifically that of FAS, and increased HDL-C, decreased TC and TG levels in the bloodstream, reduced saturated fatty acids (SFA), and increased oleic and ALA contents in rat adipose tissues indicating that HBF consumption does not pose significant risks of cardiovascular disease.

Orally administered octacosanol improves liver insulin resistance in high-fat diet-fed mice through the reconstruction of the gut microbiota structure and inhibition of the TLR4/NF-κB inflammatory pathway

“…39 Srebp-1c plays a central role in lipogenesis, enhancing the transcription of genes required for fatty acid syntheses, including fat acid synthase (Fas) and acetyl-CoA carboxylase 1 (Acc1). 40 Fas is a key enzyme controlling the rate of fatty acid synthesis, 41 while Acc1 is the rate-limiting enzyme in the biogenesis of long-chain fatty acids and regulation of fatty acid synthesis. 42 Our results showed that compared to the HFD mice, the Octa-treated mice showed significantly altered mRNA expressions of the above-mentioned genes, revealing the possibility of altered lipid metabolism through changes in the lipogenic genes caused by Octa in the HFD-fed mice.…”

Effects of Dietary Protein Concentration on Lipid Metabolism Gene Expression and Fatty Acid Composition in 18–23-Month-Old Hanwoo Steers

“…On the other hand, high total fatty acids and oleic acid contents are positively correlated with flavor [ 26 ], customer preference over imported beef [ 27 ], and favorable health characteristics for consumers [ 28 , 29 ]. Rats that eat Hanwoo beef fat show high feed intake but reduced lipogenic enzyme activities and increased high-density lipoprotein cholesterol, indicating a lower risk for cardiovascular disease compared to consuming Angus beef fat [ 30 ].…”