Oleic Acid Reduces Oxidant Stress in Cultured Pulmonary Artery Endothelial Cells

Hart, C. Michael; Gupta, Mahesh P.; Evanoff, Van

doi:10.3109/01902149709039235

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…Studies have suggested that saturated and unsaturated fatty acids have different effects on toxicity. For example, there has been evidence indicating that dietary oleic acid can protect endothelial cells against hydrogen peroxide-induced oxidative stress and reduce the susceptibility of LDLs to oxidative modifications (23)(24)(25). Similarly, we found that oleic acid does not induce the same level of cytotoxicity as palmitic acid in HepG2 cells at similar concentrations (12) and the addition of oleic acid reduces the cytotoxicity induced by palmitate (26).…”

Section: Introductionsupporting

confidence: 63%

Experimental and Computational Studies Investigating Trehalose Protection of HepG2 Cells from Palmitate-Induced Toxicity

Leekumjorn

Sum

et al. 2008

Biophysical Journal

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Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress conditions, also protects hepatocytes from palmitate-induced toxicity. Our results suggest that trehalose serves as a free radical scavenger and alleviates damage from hydrogen peroxide secreted by the compromised cells. We also observe that trehalose protects HepG2 cells by interacting with the plasma membrane to counteract the changes in membrane fluidity induced by palmitate. The experimental results are supported by molecular dynamics simulations of model cell membranes that closely reflect the experimental conditions. Simulations were performed to understand the specific interactions between lipid bilayers, palmitate, and trehalose. The simulations results reveal the early stages of how palmitate induces biophysical changes to the cellular membrane and the role of trehalose in protecting the membrane structure.

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Section: Introductionsupporting

confidence: 63%

Experimental and Computational Studies Investigating Trehalose Protection of HepG2 Cells from Palmitate-Induced Toxicity

Leekumjorn

Sum

et al. 2008

Biophysical Journal

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“…Indeed, decreased vascular inflammation and oxidative stress may be the mechanism by which the oil mixture treatment attenuates endothelial dysfunction in aged rats, as it significantly reduced the arterial expression of pro-inflammatory enzymes like COX-2 and prooxidant enzymes like NOX-4. Similarly, other authors have reported that both omega-3 and MUFA fatty acids supplementation exerts antioxidant and anti-inflammatory effects in arterial cells or tissue [ 79 , 83 ].…”

Section: Discussionmentioning

confidence: 70%

A Mixture of Algae and Extra Virgin Olive Oils Attenuates the Cardiometabolic Alterations Associated with Aging in Male Wistar Rats

et al. 2020

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Aging is one of the major risk factors for suffering cardiovascular and metabolic diseases. Due to the increase in life expectancy, there is a strong interest in the search for anti-aging strategies to treat and prevent these aging-induced disorders. Both omega 3 polyunsaturated fatty acids (ω-3 PUFA) and extra virgin olive oil (EVOO) exert numerous metabolic and cardiovascular benefits in the elderly. In addition, EVOO constitutes an interesting ingredient to stabilize ω-3 PUFA and decrease their oxidation process due to its high content in antioxidant compounds. ω-3 PUFA are commonly obtained from fish. However, more ecological and sustainable sources, such as algae oil (AO) can also be used. In this study, we aimed to study the possible beneficial effect of an oil mixture composed by EVOO (75%) and AO (25%) rich in ω-3 PUFA (35% docosahexaenoic acid (DHA) and 20% eicosapentaenoic acid (EPA)) on the cardiometabolic alterations associated with aging. For this purpose; young (three months old) and old (24 months old) male Wistar rats were treated with vehicle or with the oil mixture (2.5 mL/kg) for 21 days. Treatment with the oil mixture prevented the aging-induced increase in the serum levels of saturated fatty acids (SFA) and the aging-induced decrease in the serum concentrations of mono-unsaturated fatty acids (MUFA). Old treated rats showed increased serum concentrations of EPA and DHA and decreased HOMA-IR index and circulating levels of total cholesterol, insulin and IL-6. Treatment with the oil mixture increased the mRNA levels of antioxidant and insulin sensitivity-related enzymes, as well as reduced the gene expression of pro-inflammatory markers in the liver and in cardiac and aortic tissues. In addition, the treatment also prevented the aging-induced endothelial dysfunction and vascular insulin resistance through activation of the PI3K/Akt pathway. Moreover, aortic rings from old rats treated with the oil mixture showed a decreased response to the vasoconstrictor AngII. In conclusion, treatment with a mixture of EVOO and AO improves the lipid profile, insulin sensitivity and vascular function in aged rats and decreases aging-induced inflammation and oxidative stress in the liver, and in the cardiovascular system. Thus, it could be an interesting strategy to deal with cardiometabolic alterations associated with aging.

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“…The consequence was an increase in resistance to derangements caused by oxidized LDL and a reduction in oxidant-mediated dysfunction [159,161]. Massaro et al [162] showed that the incubation of oleate with cytokine-stimulated endothelial cells prevents the depletion of glutathione (GSH) and partially prevents stimuli-induced increase of intracellular ROS.…”

Section: Effects Of Oleic Acidmentioning

confidence: 98%

“…Therefore, an alteration of H 2 O 2 metabolism in vascular cells may contribute to the ability of fatty acids to modulate cellular oxidant susceptibility [159].…”

Section: Effects Of Oleic Acidmentioning

confidence: 99%

The role of virgin olive oil components in the modulation of endothelial function

Perona

Cabello-Moruno

Ruı́z-Gutı́errez

2006

The Journal of Nutritional Biochemistry

243

152

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The endothelium is involved in many of the processes related to the development of atherosclerosis, which is considered an inflammatory disease. Actually, traditional risk factors for atherosclerosis predispose to endothelial dysfunction, which is manifested as an increase in the expression of specific cytokines and adhesion molecules. There are firm evidence supporting the beneficial effects of olive oil, the most genuine component of the Mediterranean diet. Although the effects of olive oil and other oleic acid-rich dietary oils on atherosclerosis and plasma lipids are well known, the roles of minor components have been less investigated. Minor components constitute only 1-2% of virgin olive oil (VOO) and are composed of hydrocarbons, polyphenols, tocopherols, sterols, triterpenoids and other components usually found in traces. Despite their low concentration, non-fatty acid constituents may be of importance because studies comparing monounsaturated dietary oils have reported different effects on cardiovascular disease. Most of these compounds have demonstrated antioxidant, anti-inflammatory and hypolipidemic properties. In this review, we summarize current knowledge on the effects of these compounds contained in VOO on vascular dysfunction and the mechanisms by which they modulate endothelial activity. Such mechanisms involve the release of nitric oxide, eicosanoids (prostaglandins and leukotrienes) and adhesion molecules, in most cases by activation of nuclear factor kappaB by reactive oxygen species.

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Oleic Acid Reduces Oxidant Stress in Cultured Pulmonary Artery Endothelial Cells

Cited by 9 publications

References 30 publications

Experimental and Computational Studies Investigating Trehalose Protection of HepG2 Cells from Palmitate-Induced Toxicity

Experimental and Computational Studies Investigating Trehalose Protection of HepG2 Cells from Palmitate-Induced Toxicity

A Mixture of Algae and Extra Virgin Olive Oils Attenuates the Cardiometabolic Alterations Associated with Aging in Male Wistar Rats

The role of virgin olive oil components in the modulation of endothelial function

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