Oleuropein and Other Polyphenols from Olive (Olea europea) for Protecting the Plant Against Pseudomonas syringae subsp. savastanoi

Capasso, Renato; Cristinzio, Gennaro; Evidente, Antonio; Visca, Carla; Iannini, C.

doi:10.1007/978-94-011-5472-7_24

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Since under other assay conditions olive and oleander isolates could multiply in olive leaf tissues (Surico, 1993), it is not clear why no living cells were recovered just a few days after inoculation. Possibly, under these assay conditions, plant factors such as polyphenols and particularly antimicrobial derivatives of the glucoside oleuropein may have been important (Fleming et al ., 1973; Capasso et al ., 1997).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Pseudomonas syringae ssp. savastanoi strains isolated from ash

1998

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Isolates of Pseudomonas syringae ssp. savastanoi from ash were examined for their ability to produce phytohormones in culture and for pathogenicity, in comparison with isolates from olive and oleander. Nineteen out of 20 ash isolates produced low levels of indole-3-acetic acid and its methyl ester but no cytokinins. In contrast, the remaining isolate, NCPPB3474, accumulated high levels of auxins and cytokinins in culture, comparable to those of olive and oleander strains. Hybridization of DNA preparations with tryptophan mono-oxygenase (iaaM) and isopentenyl transferase (ipt) gene-containing probes showed sequences of DNA homologous to both probes only in isolate NCPPB3474, and in which the iaaM and ipt genes were located on the chromosome and on a plasmid of about 80 kb, respectively. When assayed for pathogenicity on ash, olive and oleander, 19 of the 20 ash isolates caused disease only on ash but NCPPB3474 caused knots on both ash and olive. Oleander isolates infected all three hosts whereas those from olive caused symptoms only on olive and ash. All the cultures were able to multiply in host plant tissues, but the growth rates and final population densities were correlated to the plant species inoculated and the host origin of the isolates. In particular, the highest population densities were reached by isolates capable of causing symptoms on the inoculated host. The phytohormone production shown by ash, olive and oleander isolates of P. syringae ssp. savastanoi was in accordance with the type of symptoms: cankers accompanied by wart-like excrescences on ash and knots on olive and oleander. Furthermore, the pathogenic features of these isolates and, in particular, their growth patterns in the different host tissues, support previous evidence on the existence of three distinct pathovars in P. syringae ssp. savastanoi.

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Section: Discussionmentioning

confidence: 99%

Characterization of Pseudomonas syringae ssp. savastanoi strains isolated from ash

1998

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“…If SA builds up in the olive knot apoplast it could result in antimicrobial activity against Psv (which is likely since Psv cannot convert SA to catechol as evidenced by Passos da Silva et al, 2014 ); this can be potentially alleviated by Et and Pa since these bacteria possess the salicylate hydroxylases gene, which converts SA to catechol. Catechol is one of the most important central intermediates in the aerobic bacterial metabolism of aromatic compounds ( Harwood and Parales, 1996 ) and it is toxic to Psv ( Capasso et al, 1997 ). This phenol may be further degraded and detoxified by the collaborative action of Psv , Et , Eo , and Pa enzymes by ortho cleavage via the 3-oxoadipate pathway, which lead in to the Krebs cycle ( Harwood and Parales, 1996 ; Figure 1 ).…”

Section: Possible Role Of the Resident Microbiotamentioning

confidence: 99%

The olive knot disease as a model to study the role of interspecies bacterial communities in plant disease

et al. 2015

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There is an increasing interest in studying interspecies bacterial interactions in diseases of animals and plants as it is believed that the great majority of bacteria found in nature live in complex communities. Plant pathologists have thus far mainly focused on studies involving single species or on their interactions with antagonistic competitors. A bacterial disease used as model to study multispecies interactions is the olive knot disease, caused by Pseudomonas savastanoi pv. savastanoi (Psv). Knots caused by Psv in branches and other aerial parts of the olive trees are an ideal niche not only for the pathogen but also for many other plant-associated bacterial species, mainly belonging to the genera Pantoea, Pectobacterium, Erwinia, and Curtobacterium. The non-pathogenic bacterial species Erwinia toletana, Pantoea agglomerans, and Erwinia oleae, which are frequently isolated inside the olive knots, cooperate with Psv in modulating the disease severity. Co-inoculations of these species with Psv result in bigger knots and better bacterial colonization when compared to single inoculations. Moreover, harmless bacteria co-localize with the pathogen inside the knots, indicating the formation of stable bacterial consortia that may facilitate the exchange of quorum sensing signals and metabolites. Here we discuss the possible role of bacterial communities in the establishment and development of olive knot disease, which we believe could be taking place in many other bacterial plant diseases.

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“…cosmetics (Proserpio & Malpede, 1995;Tanaka, 2001;Che et al, 2006), cosme-nutraceuticals (Cristoni & Morazzoni, 2003;Rull Prous et al, 2005;Mazzio, 2007), functional food (Osakabe, 2006;Yamada, 2007) and medicine (Ishimaru & Shimomura, 1995;Capasso et al, 1997;Metz, 2000;Liu & Henkel, 2002).…”

Section: Conclusion and Perspectives Of Plantago In Vitro Culturesmentioning

confidence: 99%

Culture ofPlantagospecies as bioactive components resources: a 20-year review and recent applications

Fons¹,

Gargadennec²,

Rapior³

2008

Acta Botanica Gallica

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Abstract.-Most of species from the worldwide distributed Plantago genus are greatly used as herbal medicines. Phytochemical investigations of various Plantago organs (leaves, stems) reveal their high potential to produce a wide array of bioactive secondary metabolites. So, in vitro cultures of Plantago species are managed in order to deliberately restrict the ecological factors and then to control the culture conditions. Experimental culture parameters of Plantago species, i.e. seed germination, temperature, relative humidity, light, substrates and additional nutritive solutions as well as the in vitro culture media and growth regulators are reviewed. The expensive optimizations of in vitro culture and biotransformation processes are reported. Changes in the concentration and the pattern of bioactive polyphenol compounds synthesized from in vitro cultures are discussed in the examined species of the genus Plantago in order to conclude about their potential interest as future drug candidates.Key words : plantain -Plantaginaceae -in vitro culture -polyphenol -biotransformation -medicine.Résumé.-La plupart des espèces du genre cosmopolite Plantago sont largement utilisées en médecine traditionnelle. Des études phytochimiques sur les différents organes des plantains (feuilles, tiges) révèlent leur fort potentiel à produire un large spectre de molécules biologiquement actives. Les techniques de cultures in vitro des espèces du genre Plantago se sont alors développées afin de restreindre les facteurs écologiques et de maîtriser les conditions culturales. Les paramètres expérimentaux de culture tels que la germination des graines, la température, l'humidité relative, la lumière, la nature des substrats et des solutions nutritives ainsi que la composition chimique des milieux de culture in vitro et les régulateurs de croissance additionnels ont été passés en revue. Les coû-teuses méthodes d'optimisation des cultures in vitro et les procédés de biotransformation ont été rapportés. Les variations qualitatives et quantitatives des biomolécules de nature polyphénolique synthétisées par cultures in vitro de Plantago ont été argumentées, espèce par espèce, afin de définir l'intérêt théra-peutique potentiel de chacune.Mots clés : plantain -Plantaginaceae -culture in vitro -polyphenol -biotransformation -médecine.

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Oleuropein and Other Polyphenols from Olive (Olea europea) for Protecting the Plant Against Pseudomonas syringae subsp. savastanoi

Cited by 4 publications

References 6 publications

Characterization of Pseudomonas syringae ssp. savastanoi strains isolated from ash

Characterization of Pseudomonas syringae ssp. savastanoi strains isolated from ash

The olive knot disease as a model to study the role of interspecies bacterial communities in plant disease

Culture ofPlantagospecies as bioactive components resources: a 20-year review and recent applications

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