Olfactory cleft mucus proteome in chronic rhinosinusitis: a case‐control pilot study

Abstract

Background Mechanisms of smell loss in chronic rhinosinusitis CRS are still unclear and likely multifactorial Little attention has been given to olfactory cle OC mucus proteins involved in odorant binding and metabolizing enzymes and their potential role in smell loss Methods Mucus from the OC was sampled from patients with CRS n = and controls n = Liquid chromatography and mass spectrometry were performed followed by data processing so that protein groups could be identified quantified and compared Hierarchical clustering and bioinformatic analysis were performed on significantly different proteins to explore for enrichment in known biologic pathways Results A total of proteins were found in OC mucus from all subjects Significant differences in protein abundance were found between CRS and controls including both CRSsNP n = proteins log fold change range to and CRSwNP n = proteins log fold change range -to -Significant differences were found between patients with normosmia and those with dysosmia n = log fold change range -to -and across groups of interest for a number of odorant binding proteins and metabolizing enzymes Conclusion OC mucous in CRS displays a rich and abundant array of proteins many of which have been implicated in odorant transport and metabolization in animal studies Significant differences in the olfactory mucus proteome were seen between CRS subtypes and controls as well as between those with normal and abnormal olfaction Further study should confirm these findings and explore the role individual proteins play in odorant transport and metabolization © ARSAAOA LLC © 2020 ARS-AAOA, LLC.

Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

“…1 Understanding the etiopathology of CRSsNP as well as developing an effective treatment remains challenging, although recent advantages have been made. [25][26][27][28][29][30] Current therapies are based on patient phenotype, and subtypes based on significant underlying immunologic differences are not considered. 31,32 Accordingly, these patients' clinical responses on medical therapy can be highly variable [33][34][35] and relapse surgery is often required.…”

Pro‐inflammatory markers associated with COVID‐19‐related persistent olfactory dysfunction

“…Olfactory cleft mucosal sampling under nasal endoscopy was performed in the clinic and a sterile filter membrane (Leukosorb, Pall Corporation) was placed into the OC bilaterally under direct visualization, as previously published. [19][20][21] After 2 min, the membrane was removed, placed directly on ice, and centrifuged for 10 min at 13,000 rpm in 4 • C. The mucus from both sides were combined, 1× protease inhibitor (Halt, Thermo Fisher) added, and stored at −20 • C prior to assay conduction.…”

Recent advances in mass spectrometry-based proteomics and metabolomics in chronic rhinosinusitis with nasal polyps

“…studied OC mucus proteins from CRSwNP, CRSsNP, and controls. They found that Lipocalin-1 and Lipocalin-15 which are thought to play a role in odorant binding, and glutathione S-transferases (GSTs), alcohol dehydrogenases, and other oxidizing enzymes, which are thought to play a role in odorant metabolism were downregulated in CRSwNP ( 28 ). Their findings suggest OC mucus dysregulation may indeed be associated with olfactory dysfunction in CRS patients.…”