Olfactory perception of herbicide butachlor by GOBP2 elicits ecdysone biosynthesis and detoxification enzyme responsible for chlorpyrifos tolerance in Spodoptera litura

Sun, Zhongxiang; Wang, Rumeng; Du, Yifei; Gao, Binyuan; Gui, Furong; Lǚ, Kai

doi:10.1016/j.envpol.2021.117409

Cited by 21 publications

(22 citation statements)

References 55 publications

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“…Olfactory recognition of the herbicide butachlor induces the biosynthesis of steroid hormone 20E and leads to enhanced chlorpyrifos tolerance in S. litura . This indicates that insecticide tolerance is also regulated by the 20E signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…Ecdysone content was determined using an Insect Ecdysone Assay Kit (Jiangsu Yutong) as previously described . The ecdysone content was expressed as ng of ecdysone per g of protein.…”

Section: Methodsmentioning

confidence: 99%

“…The concentration of dsRNA was quantified at 260 nm and adjusted to 4 μg/μL. An injection-based method was used for dsRNA delivery as previously reported . Briefly, the fourth instar larvae were injected with 8 μg of mixed dsRNA (1 μL of dsEcR and 1 μL of dsUSP, dsMix), while the control larvae were injected with an equivalent volume of dsGFP (2 μL of 4 μg/μL) using a microinjector (High Pigeon Industry and Trade Co., Ltd., Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…A regulatory relationship between the ROS/CncC pathway and the downstream 20-hydroxyecdysone (20E) synthesis was observed in Bombyx mori . Olfactory recognition of the herbicide butachlor triggered 20E biosynthesis and led to chlorpyrifos tolerance in S. litura . These reports suggest that the ROS/CncC signaling pathway may work through 20E signaling cascade to regulate metabolic detoxification associated with insecticide tolerance in response to phytochemicals.…”

Section: Introductionmentioning

confidence: 99%

“…25 Olfactory recognition of the herbicide butachlor triggered 20E biosynthesis and led to chlorpyrifos tolerance in S. litura. 26 These reports suggest that the ROS/CncC signaling pathway may work through 20E signaling cascade to regulate metabolic detoxification associated with insecticide tolerance in response to phytochemicals. However, this hypothesis is not validated, and direct evidence of the signaling cascades and insect hormones underlying phytochemical induction of detoxification genes is needed.…”

Section: ■ Introductionmentioning

confidence: 99%

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Activation of the ROS/CncC and 20-Hydroxyecdysone Signaling Pathways Is Associated with Xanthotoxin-Induced Tolerance to λ-Cyhalothrin in Spodoptera litura

Lǚ

Cheng

et al. 2021

J. Agric. Food Chem.

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Adaptation to phytochemicals in herbivorous insects can influence tolerance to insecticides. However, it is unclear how insects use phytochemicals as cues to activate their metabolic detoxification systems. In this study, we found that dietary exposure to xanthotoxin enhanced tolerance of Spodoptera litura larvae to λ-cyhalothrin. Xanthotoxin ingestion significantly elevated the mRNA levels of 35 detoxification genes as well as the transcription factors Cap ‘n’ collar isoform-C (CncC) and its binding factor small muscle aponeurosis fibromatosis isoform-K (MafK). Additionally, xanthotoxin exposure increased the levels of reactive oxygen species (ROS), while ROS inhibitor N-acetylcysteine (NAC) treatment blocked xanthotoxin-induced expression of CncC, MafK, and detoxification genes and also prevented xanthotoxin-enhanced larval tolerance to λ-cyhalothrin. The 20-hydroxyecdysone (20E) signaling pathway was effectively activated by xanthotoxin, while blocking of 20E signaling transduction prevented xanthotoxin-enhanced larval tolerance to λ-cyhalothrin. Application of 20E induced the expression of multiple xanthotoxin-induced detoxification genes and enhanced λ-cyhalothrin tolerance in S. litura. NAC treatment blocked xanthotoxin-induced 20E synthesis, while the CncC agonist curcumin activated the 20E signaling pathway. These results indicate that the ROS/CncC pathway controls the induction of metabolic detoxification upon exposure to xanthotoxin, at least in part, through its regulation of the 20E signaling pathway.

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Section: Discussionmentioning

confidence: 99%

“…Ecdysone content was determined using an Insect Ecdysone Assay Kit (Jiangsu Yutong) as previously described . The ecdysone content was expressed as ng of ecdysone per g of protein.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Activation of the ROS/CncC and 20-Hydroxyecdysone Signaling Pathways Is Associated with Xanthotoxin-Induced Tolerance to λ-Cyhalothrin in Spodoptera litura

Lǚ

Cheng

et al. 2021

J. Agric. Food Chem.

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Cross‐protection interactions in insect pests: Implications for pest management in a changing climate

Bueno

McIlhenny

Chen

2022

Pest Management Science

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Agricultural insect pests display an exceptional ability to adapt quickly to natural and anthropogenic stressors. Emerging evidence suggests that frequent and varied sources of stress play an important role in driving protective physiological responses; therefore, intensively managed agroecosystems combined with climatic shifts might be an ideal crucible for stress adaptation. Cross‐protection, where responses to one stressor offers protection against another type of stressor, has been well documented in many insect species, yet the molecular and epigenetic underpinnings that drive overlapping protective responses in insect pests remain unclear. In this perspective, we discuss cross‐protection mechanisms and provide an argument for its potential role in increasing tolerance to a wide range of natural and anthropogenic stressors in agricultural insect pests. By drawing from existing literature on single and multiple stressor studies, we outline the processes that facilitate cross‐protective interactions, including epigenetic modifications, which are understudied in insect stress responses. Finally, we discuss the implications of cross‐protection for insect pest management, focusing on the consequences of cross‐protection between insecticides and elevated temperatures associated with climate change. Given the multiple ways that insect pests are intensively managed in agroecosystems, we suggest that examining the role of multiple stressors can be important in understanding the wide adaptability of agricultural insect pests. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Functional characterization of CYP6AE subfamily P450s associated with pyrethroid detoxification in Spodoptera litura

Xiao

Lǚ²

2022

International Journal of Biological Macromolecules

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Olfactory perception of herbicide butachlor by GOBP2 elicits ecdysone biosynthesis and detoxification enzyme responsible for chlorpyrifos tolerance in Spodoptera litura

Cited by 21 publications

References 55 publications

Activation of the ROS/CncC and 20-Hydroxyecdysone Signaling Pathways Is Associated with Xanthotoxin-Induced Tolerance to λ-Cyhalothrin in Spodoptera litura

Activation of the ROS/CncC and 20-Hydroxyecdysone Signaling Pathways Is Associated with Xanthotoxin-Induced Tolerance to λ-Cyhalothrin in Spodoptera litura

Cross‐protection interactions in insect pests: Implications for pest management in a changing climate

Functional characterization of CYP6AE subfamily P450s associated with pyrethroid detoxification in Spodoptera litura

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