Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility

Schleicher, Martina; Hansmann, Jan; Elkin, Bentsian; Kluger, Petra J.; Liebscher, Simone; Huber, Agnes J.; Fritze, Olaf; Schille, Christine; Müller, Michaela; Schenke‐Layland, Katja; Seifert, Martina; Walles, Heike; Wendel, Hans-Peter; Stock, Ulrich A.

doi:10.1155/2012/397813

Cited by 19 publications

(28 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14 In addition, a study by Schleicher et al showed that PC exhibits low thrombogenicity and excellent hemocompatibility after oligonucleotide coating. 13 In the current study, we showed that cellular morphology, phenotype, and viability of PVECs and PVICs grown on plasma-treated PC showed similar patterns to cells grown in normal in vitro conditions.…”

Section: Discussionsupporting

confidence: 58%

“…The ability of the scaffold material to adsorb ECM proteins is important in providing such an environment. 13 In addition, surface wettability is an important factor in enhancing cell adhesion and protein adsorption. 23 In this study, we applied two surface modifications to PC: first, we plasma oxidized PC to induce its hydrophilicity, and then we coated the material with a range of concentrations of ECM proteins to provide a favorable environment for cellular adhesion and growth.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies had investigated the biocompatibility of PC with the growth of different cell types, such as fibroblasts (NIH 3T3), hepatocytes, 12 human umbilical vain endothelial cells, 13 and retinal pigment epithelial cells. 14 In addition, a study by Schleicher et al showed that PC exhibits low thrombogenicity and excellent hemocompatibility after oligonucleotide coating.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment Of Parylene C Thin Films For Heart Valve Tissue Engineering

Marei

2013

Qatar Foundation Annual Research Forum Volume 2013 Issue 1

View full text Add to dashboard Cite

Background: Scaffolds are a key component of tissue-engineered heart valves (TEHVs). Several approaches had been adopted in the design of scaffolds using both natural and synthetic resources. We have investigated the suitability of parylene C (PC), a vapor deposited polymeric material, for the use as a scaffold in TEHV. Aims: To evaluate the adsorption of extracellular matrix components onto plasma-activated PC and study the biocompatibility of PC by measuring cellular adhesion, viability, apoptosis, and phenotypic expression of valve endothelial and interstitial cells. Finally, the mechanical properties of PC were compared with those of native aortic valve cusp tissue. Methods: PC slides were plasma activated and then coated with gelatin, type I collagen, or fibronectin. Porcine pulmonary valve endothelial and interstitial cells were then grown on plasma oxidized PC with different types of coatings and their adhesion was observed after 20 h of incubation. Cell viability was tested using the MTS assay, and apoptosis was estimated using TUNEL staining. The mechanical properties of PC and valve tissue were measured using a Bose Mechanical Tester. Finally, cell-seeded PC films were exposed to pulsatile pressure and aortic shear stress, respectively, to test their durability in a dynamic environment. Results: Our findings show that collagen and fibronectin could bind to plasma oxidized PC. Both valve endothelial and interstitial cells adhered to protein-coated ECM. PC had a profile of mechanical stiffness and ultimate tensile strength that were comparable with or in excess of those seen in porcine aortic valve cusps. Cells were still attached to PC films after 3 days of exposure to up to 50 mmHg pulsatile pressure or aortic levels of shear stress. Conclusion: PC is a promising candidate for use as a scaffold in tissue engineering heart valves. Additional studies are required to determine both the durability and long-term performance of cell-seeded PC when in a similar hemodynamic environment to that of the aortic valve.

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment Of Parylene C Thin Films For Heart Valve Tissue Engineering

Marei

2013

Qatar Foundation Annual Research Forum Volume 2013 Issue 1

View full text Add to dashboard Cite

show abstract

“…The aptamer-coated polymeric surfaces were able to promote the selective adhesion of EPCs followed by their differentiation into endothelial cells after ten days. Similarly, in a more recent study, DNA oligonucleotides were immobilized on different vascular grafts made of expanded polytetrafluoroethylene (ePTFE) and polystyrene to promote in situ endothelialization [131]. The coated surfaces allowed adhesion of murine EPCs and showed low thrombogenicity and improved hemato-compatibility with human blood.…”

Section: Cardiovascular Regeneration Using Stem Cell Recruitment Smentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

132

View full text Add to dashboard Cite

A leading strategy in tissue engineering is the design of biomimetic scaffolds that stimulate the body’s repair mechanisms through the recruitment of endogenous stem cells to sites of injury. Approaches that employ the use of chemoattractant gradients to guide tissue regeneration without external cell sources are favored over traditional cell-based therapies that have limited potential for clinical translation. Following this concept, bioactive scaffolds can be engineered to provide a temporally and spatially controlled release of biological cues, with the possibility to mimic the complex signaling patterns of endogenous tissue regeneration. Another effective way to regulate stem cell activity is to leverage the inherent chemotactic properties of extracellular matrix (ECM)-based materials to build versatile cell-instructive platforms. This review introduces the concept of endogenous stem cell recruitment, and provides a comprehensive overview of the strategies available to achieve effective cardiovascular and bone tissue regeneration.

show abstract

“…85 In a recent study, DNA-oligonucleotide coatings were immobilized by adsorption on parylenecoated ePTFE and polystyrene using vapor deposition. 86 Murine EPCs, HUVECs, and adult human ECs were seeded. Dynamic culture conditions were maintained to apply constant shear stress.…”

mentioning

confidence: 99%