Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces

Левина, А. С.; Pyshnaya, I. A.; Репкова, М. Н.; Рар, В. А.; Зарытова, В. Ф.

doi:10.1002/biot.200700027

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…It would therefore be desirable to link DNA directly to unmodified glass, preferably by a one-step procedure. Both Kumar et al (8) and Levina et al (14) linked DNA to glass by adding a silane group or a polylysine group at the end of each DNA probe, respectively. Modifying each oligo with different chemical groups is, however, difficult as well as expensive.…”

Section: Introductionmentioning

confidence: 98%

An Inexpensive and Simple Method for Thermally Stable Immobilization of DNA on an Unmodified Glass Surface: UV Linking of Poly(T)10-poly(C)10–Tagged DNA Probes

et al. 2008

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Microarrays printed on glass slides are often constructed by covalently linking modified oligonucleotide probes to a derivatized surface at considerable expense. In this article, we demonstrate that 14-base oligonucleotides with a poly(T)10 - poly(C)10 tail (TC tag), but otherwise unmodified, can be linked by UV light irradiation onto a plain, unmodified glass surface. Probes immobilized onto unmodified glass microscope slides performed similarly to probes bound to commercial amino-silane-coated slides and had comparable detection limits. The TC-tagged probes linked to unmodified glass did not show any significant decrease in hybridization performance after a 20 min incubation in water at 100 degrees C prior to rehybridization, indicating a covalent bond between the TC tag and unmodified glass. The probes were used in thermal minisequencing cycling reactions. Furthermore, the TC tag improved the hybridization performance of the immobilized probes on the amino-silane surface, indicating a general benefit of adding a TC tag to DNA probes. In conclusion, our results show that using TC-tagged DNA probes immobilized on an unmodified glass surface is a robust, heat-stable, very simple, and inexpensive method for manufacturing DNA microarrays.

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Section: Introductionmentioning

confidence: 98%

An Inexpensive and Simple Method for Thermally Stable Immobilization of DNA on an Unmodified Glass Surface: UV Linking of Poly(T)10-poly(C)10–Tagged DNA Probes

et al. 2008

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“…Here we used nanocomposites based on one form of nanoparticles (anatase). PL-DNA conjugates30 were immobilized on anatase to give TiO 2 ·PL-DNA bearing 20 nmol of DNA per 1 mg of anatase.…”

Section: Resultsmentioning

confidence: 99%

High-performance method for specific effect on nucleic acids in cells using TiO2~DNA nanocomposites

Левина

Репкова

Ismagilov

et al. 2012

Sci Rep

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Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO2·PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO2 nanoparticles capable of transferring DNA). These nanocomposites were used for inhibition of human influenza A (H3N2) virus replication in infected MDCK cells. They showed a low toxicity (TC50 ≈ 1800 μg/ml) and a high antiviral activity (>99.9% inhibition of the virus replication). The specificity factor (antisense effect) appeared to depend on the delivery system of DNA fragments. This factor for nanocomposites is ten-times higher than for DNA in the presence of lipofectamine. IC50 for nanocomposites was estimated to be 1.5 μg/ml (30 nM for DNA), so its selectivity index was calculated as ~1200. Thus, the proposed nanocomposites are prospective for therapeutic application.

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“…The authors of this review developed the methods of immobilization of oligonucleotides on commercial silica nanoparticles (10-20 nm) [62]. Polylysine (PL) or polylysine conjugates with an oligonucleotide (PL-ODN) were immobilized on silanized nanoparticles due to the affinity of polylysine to various surfaces [63] (Scheme 8A, reaction 1) PL and PL-ODN were attached to the nanoparticles bearing epoxy groups by the reaction with the amine groups of polylysine (Scheme 8A, reaction 2) Oligonucleotides were immobilized on the amino-containing nanoparticles due to the electrostatic interaction between the amino groups in the nanoparticles and internucleotide phosphate groups (Scheme 8A, reaction 3). It should be noted that in the cases represented by reactions 1a, 1b, 2a, and 3, oligonucleotides or their conjugates with polylysine are attached to nanoparticles noncovalently.…”

Section: Modification Of Commercial Silica Nanoparticlesmentioning

confidence: 99%

Methods of the Synthesis of Silicon-Containing Nanoparticles Intended for Nucleic Acid Delivery

Левина¹,

Репкова²,

Ismagilov³

et al. 2018

Eurasian Chem. Tech. J.

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A promising new approach to the treatment of viral infections and genetic diseases associated with damaged or foreign nucleic acids in the body is gene therapy, i.e., the use of antisense oligonucleotides, ribozymes, deoxyribozymes, siRNA, plasmid DNA, etc. (therapeutic nucleic acids). Selective recognition of target nucleic acids by these compounds based on highly specific complementary interaction can minimize negative side effects, which occur with currently used low molecular weight drugs. To apply a new generation of therapeutic agents in medical practice, it is necessary to solve the problem of their delivery into cells. Silicon-containing nanoparticles are considered as promising carriers for this purpose due to their biocompatibility, low toxicity, ability to biodegradation and excretion from the body, as well as the simplicity of the synthesis and modification. Silicon-containing nanoparticles are divided into two broad categories: solid (nonporous) and mesoporous silicon nanoparticles (MSN). This review gives a brief overview of the creation of mesoporous, multilayer, and other silicon-based nanoparticles. The publications concerning solid silicon-organic nanoparticles capable of binding and delivering nucleic acids into cells are discussed in more detail with emphasis on methods for their synthesis. The review covers publications over the past 15 years, which describe the classical Stöber method, the microemulsion method, modification of commercial silica nanoparticles, and other strategies.

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Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces

Cited by 7 publications

References 32 publications

An Inexpensive and Simple Method for Thermally Stable Immobilization of DNA on an Unmodified Glass Surface: UV Linking of Poly(T)10-poly(C)10–Tagged DNA Probes

An Inexpensive and Simple Method for Thermally Stable Immobilization of DNA on an Unmodified Glass Surface: UV Linking of Poly(T)10-poly(C)10–Tagged DNA Probes

High-performance method for specific effect on nucleic acids in cells using TiO2~DNA nanocomposites

Methods of the Synthesis of Silicon-Containing Nanoparticles Intended for Nucleic Acid Delivery

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