Oligonucleotides from Yeast Tyrosine Transfer Ribonucleic Acid

Oligonucleotide composition of a yeast lysine transfer ribonucleic acid

“…Up to 12 g of bulk tRNA was dissolved in 800 ml of the ammonium sulfate system and distributed for 225 transfers at 25 = 1 °. The faster moving tR NALys peak was removed from the countercurrent system with CetMe3NBr* 1 (Eastman, technical) as previously described (Madison et al, 1967), except that eight tubes from the countercurrent were combined (640 ml), and CetMe3NBr equal to about ten times the weight of RNA present was added. After the addition of diethyl ether, the gelatinous RNA-methyluridine methyl ester in the first position of the anticodon.…”

“…The columns were run at room temperature. The tubes could be assayed directly for lysine acceptor activity (Madison et al, 1967). The peaks of tRNALys were combined, enough CetMesNBr was added to make the CetMe3NBr :RNA ratio about 150, and the RNA was isolated as above.…”

“…Analyses. Nuclease digestions, isolation of oligonucleotides, and analysis of nucleotide composition have all been described (Madison et al, 1967) except that the chromatography on DEAE-cellulose in 7 m urea was done in the presence of 0.02 m morpholinopropanesulfonic acid (pH 7.0). The columns were run at room temperature with a flow rate of about 15 ml/hr.…”

Localization ofN6-(Δ2-isopentenyl)adenosine among the acceptor species of transfer ribonucleic acid of Lactobacillus acidophilus

“…The sequences underlined (third through seventh base) correspond to a common sequence in some of the tRNAs. The sequences shown are from the following citations: tyrosine (yeast), Madison et al (1967), serine (yeast), Zachau et al (1966); phenylalanine (yeast), Raj-Bhandary et al (1967); tyrosine (E. coli), Goodman et al (1968); phenylalanine {E. coli), Uziel and Gassen (1969); serine (rat liver),…”

Stimulation of incorporation of nucleic acid precursors into HeLa cells caused by provaline

“…of pancreatic ribonuclease in 5ml. of 01IM-tris-HCI buffer, pH7-5 (Madison, Everett & King, 1967). The hydrolysate was shaken with an equal volume of aq.…”