Oligosaccharides induce changes in protein patterns of regenerating spruce protoplasts

Kákoniová, D.; Hlinková, E.; Lišková, Desana; Kollárová, Karin

doi:10.2478/s11535-010-0018-0

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…Galactoglucomannan oligosaccharides (GGMOs) derived from plant cell walls galactoglucomannan influence growth, developmental processes, and defence reactions in plant cells [ 16 – 18 ]. GGMOs showed inhibition effect on elongation growth of pea and spruce stem segments induced by auxins and gibberellin at very low concentrations [ 19 , 20 ] and their inhibitory effect depended on their chemical structure [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Growth and Anatomical Parameters of Adventitious Roots Formed on Mung Bean Hypocotyls Are Correlated with Galactoglucomannan Oligosaccharides Structure

Kollárová

Zelko

Henselová

et al. 2012

The Scientific World Journal

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The effect of galactoglucomannan oligosaccharides (GGMOs) compared with chemically modified oligosaccharides, GGMOs-g (with reduced number of D-galactose side chains) and GGMOs-r (with reduced reducing ends) on mung bean (Vigna radiata (L.) Wilczek) adventitious roots formation, elongation, and anatomical structure have been studied. All types of oligosaccharides influenced adventitious root formation in the same way: stimulation in the absence of exogenous auxin and inhibition in the presence of exogenous auxin. Both reactions are probably related with the presence/content of endogenous auxin in plant cuttings. However, the adventitious root length was inhibited by GGMOs both in the absence as well as in the presence of auxin (IBA or NAA), while GGMOs-g inhibition was significantly weaker compared with GGMOs. GGMOs-r were without significant difference on both processes, compared with GGMOs. GGMOs affected not only the adventitious root length but also their anatomy in dependence on the combination with certain type of auxin. The oligosaccharides influenced cortical cells division, which was reflected in the cortex area and in the root diameter. All processes followed were dependent on oligosaccharides chemical structure. The results suggest also that GGM-derived oligosaccharides may play an important role in adventitious roots elongation but not in their formation.

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Section: Introductionmentioning

confidence: 99%

Growth and Anatomical Parameters of Adventitious Roots Formed on Mung Bean Hypocotyls Are Correlated with Galactoglucomannan Oligosaccharides Structure

Kollárová

Zelko

Henselová

et al. 2012

The Scientific World Journal

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“…2a-d). Qualitative and quantitative differences between Glu indicate on the changes in the enzymes activity connected with different intensity of biosynthesis of saccharides and oligosaccharides [22]. Biosynthesis pathways of saccharides and oligosaccharides are connected with activity of additional enzymes as b-glucosidases, manases, galactosidases and galactomanases ( Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…b-1,3-glucanases were identified directly on the slab gels [17]. Peroxidases were identified according to [14,22]. SDS (sodium dodecyl sulphate)-PAGE was done according to Laemmli [18].…”

Section: Protein Isolation and Separationmentioning

confidence: 99%

Influence of the Environment on the Morphological and Biochemical Characteristics of Different Aspergillus niger Wild Type Strains

et al. 2012

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The present work studied the differences in accumulation, transformation and volatilization of different heavy metals ions on molecular and macromorphological features of Aspergillus niger wild type strains. Four different strains of A. niger (An) were used. Three strains (An-P, An-N, An-S) were isolated from acid and ultra acid mining regions with higher concentration of As and Sb. The fourth strain (An-G) was used as the comparative one. Environmental burden strongly affected biochemical, macro and micromorphological characteristics of studied strains. The RAMP profiles showed 90 % similarity among the studied strains. The strain An-S showed its own characteristic RAMP profile, different to the others ones. Analyzed strains can be clustered into two groups on the basis of the changes in gene expression and morphological parameters. Differences were found in both acid b-1,3-glucanases and peroxidases. Main quantitative and qualitative differences by A-PAGE and SDS-PAGE were registered for proteins with Mr * 50; 34; 28-27 and 11 kDa. Presence of living mutants of A. niger strains in old environmental burden indicate on the adaptation and mutation processes of soil microorganisms from the point of long-term effect.

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“…Galactoglucomannan-derived oligosaccharides are signaling molecules in plant cell elongation and differentiation. They positively influenced protoplast viability and regeneration (Kakoniova et al 2010). Zingiber officinale SC Liquid 10-50 P Guo et al (2007b) Polyamines are involved in a variety of growth and developmental processes in higher plants, and in stress responses.…”

Section: Rationalization Of Regenerationmentioning

confidence: 99%

Progress in plant protoplast research

Eeckhaut¹,

Lakshmanan

Deryckere³

et al. 2013

Planta

135

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Oligosaccharides induce changes in protein patterns of regenerating spruce protoplasts

Cited by 7 publications

References 36 publications

Growth and Anatomical Parameters of Adventitious Roots Formed on Mung Bean Hypocotyls Are Correlated with Galactoglucomannan Oligosaccharides Structure

Growth and Anatomical Parameters of Adventitious Roots Formed on Mung Bean Hypocotyls Are Correlated with Galactoglucomannan Oligosaccharides Structure

Influence of the Environment on the Morphological and Biochemical Characteristics of Different Aspergillus niger Wild Type Strains

Progress in plant protoplast research

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