Oligosaccharidic fractions derived from Triticum vulgare extract accelerate tissutal repairing processes in in vitro and in vivo models of skin lesions

Sanguigno, Luca; Minale, Massimiliano; Vannini, E; G, Arató; Riccio, Rodolfo; Casapullo, Agostino; Monti, Maria Chiara; Riccio, Raffaele; Formisano, Silvestro; Renzo, Gianfranco Di; Cuomo, Ornella

doi:10.1016/j.jep.2014.10.051

Cited by 24 publications

(28 citation statements)

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“…The batch number for the seeds used for the present paper was 12/001-B10148/201/04. Triticum vulgare extract (TVE-DAMOR) is a specific aqueous extract of Triticum vulgare, obtained by a complex and specific process as already described [24]. It was a gift of Farmaceutici Damor (Naples, Italy).…”

Section: Methodsmentioning

confidence: 99%

Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing

Funel

Dini

Janowska

et al. 2020

Mediators of Inflammation

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Matrix metalloproteinases (MMPs) are a large family of ubiquitously expressed zinc-dependent enzymes with proteolitic activities. They are expressed in physiological situations and pathological conditions involving inflammatory processes including epithelial to mesenchymal transition (EMT), neuronal injury, and cancer. There is also evidence that MMPs regulate inflammation in tumor microenvironment, which plays an important role in healing tissue processes. Looking at both inflammatory and neuronal damages, MMP9 is involved in both processes and their modulation seems to be regulated by two proteins: tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6). However other important genes are involved in molecular regulation of transcription factors, protein-kinase B (AKT), and p65. In addition, Triticum vulgare extract (TVE) modulated the biological markers associated with inflammatory processes, including p65 protein. While there are no evidence that TVE might be involved in the biological modulation of other inflammatory marker as AKT, we would like to assess whether TVE is able to (1) modulate phosphorylation of AKT (pAKT) as an early marker of inflammatory process in vitro and (2) affect MMP9 protein expression in an in vitro model. The BV-2 cells (microglial of mouse) have been used as an in vitro model to simulate both inflammatory and neuronal injury pathologies. Here, MMP9 seems to be involved in cellular migration through inflammatory marker activation. We simulate an inflammatory preclinical model treating BV-2 cells with lipopolysaccharide (LPS) to induce proinflammatory activation affecting pAKT and p65 proteins. TVE is revealed to restore the native expression of AKT and p65. Additionally, TVE extract modulates also the protein concentration of MMP9. Nevertheless, immunofluorescence confocal analyses revealed that both AKT and MMP9 are regulated together, synchronously. This work seems to demonstrate that two important genes can be used to monitor the beginning of an inflammatory process, AKT and MMP9, in which TVE seems able to modulate their expression of inflammation-associated molecules.

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Section: Methodsmentioning

confidence: 99%

Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing

Funel

Dini

Janowska

et al. 2020

Mediators of Inflammation

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“…It enhances chemotaxis and fibroblast maturation by synthesizing collagen fibers and glycosaminoglycans. 30 In the daily practice of medicine, this drug is particularly used in cases having skin changes and wounds sec-7tepeklinik ondary to radiation, eczema and psoriasis-like symptoms, burns at every grade, persistent wounds, occupational skin disorders, cracks due to cold, and in cases where renewal of skin tissue slows or changes due to various reasons. 30,31 d) Chamomile Chamomile has been used as a herbal medication for ages.…”

Section: ) Herbal Agents A) Centella Asiatica Extract (Gotu Cola)mentioning

confidence: 99%

Drugs used in the treatment of oral mucosal diseases

Karapinar¹,

Ünür²

2019

Yeditepe J Dent

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The oral mucosa and the surrounding tissues are the entrance to the body, where oral mucosa-specific lesions can be seen, and where symptoms or signs of many systemic diseases can be observed. The etiology of the lesions occurring in the oral mucosa may be trauma, infection, immunological causes or idiopathic. Drug application onto the mucosa covering the oral cavity has long been a field of pharmacology. Lesions that occur as a symptom of systemic diseases are usually seen as ulcerations. The purpose of the treatment is to apply symptomatic treatments that mainly improve the patient's comfort while solving the underlying problem. These are achieved primarily by correcting oral hygiene, regulating the diet, and prescribing medicines in the appropriate manner mentioned in the article. In this review, we aimed to review the drugs used in oral mucosal diseases by the dentists who frequently deal with them.

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“…[5][6][7] Among them, Triticum vulgare has been extensively used in traditional medicine thanks to its properties of accelerating tissue repair. In particular, an aqueous extract of Triticum vulgare, with bioinductive properties, obtained from the whole-germinated plants according to a procedure already described by Sanguigno (2015) and patented (Riccio, 2012), was very helpful in accelerating the wound healing process acting through different mechanisms. 8,9 It was demonstrated that the extract of Triticum vulgare was able to induce the proliferation of fibroblasts and of endothelial cells giving an acceleration to wound repair.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, an aqueous extract of Triticum vulgare, with bioinductive properties, obtained from the whole-germinated plants according to a procedure already described by Sanguigno (2015) and patented (Riccio, 2012), was very helpful in accelerating the wound healing process acting through different mechanisms. 8,9 It was demonstrated that the extract of Triticum vulgare was able to induce the proliferation of fibroblasts and of endothelial cells giving an acceleration to wound repair. [10][11][12][13] This was confirmed by studies on animal models where it was demonstrated that Triticum vulgare extract regenerated skin lesions.…”

Section: Introductionmentioning

confidence: 99%

<p>A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process</p>

Tito¹,

Minale²,

Riccio³

et al. 2020

CCID

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Introduction: Chronic skin lesions represent a problem of increasing occurrence, mostly due to the global ageing of the world population. Research in skin care and dermatology is constantly looking for new non-invasive solutions, preferably those based on the use of natural certified products, able to accelerate the spontaneous skin repair mechanisms and without altering the skin normal appearance and functionality. The wound healing process in the skin is finely regulated by several factors and orchestrated mechanisms, which modulate the progression and the fitting of different consequent phases, including haemostasis, inflammation, cell proliferation and tissue remodelling. It was previously shown that a patented Triticum vulgare aqueous extract was able to trigger the skin repair process by stimulating new tissue growth and reducing the expression levels of inflammatory mediators, such as IL-6, TNFα, prostaglandin E2, and nitric oxide. Methods: Scratch assay was performed in Human Dermal Fibroblasts (HDF). The production of fibronectin was measured by gene expression, protein quantification and localization using specific antibodies in HDF. The polymerization of actin was measured using rhodaminphalloidin in HDF. The epidermal lipid content was estimated in HaCaT (human spontaneously immortalized keratinocytes) using Nile Red staining and the increasing GBA gene expression and activity was demonstrated by RT-PCR and enzymatic activity assay. Results: In the present study, it was demonstrated that the T. vulgare extract enhanced cell migration inducing the synthesis of fibronectin, new actin polymerization and stimulating the expression of the Hyaluronan Synthase 2. Furthermore it improved the restoration of the epidermal barrier stimulating lipid synthesis. Conclusion: In conclusion, we demonstrated that the T. vulgare extract possessed promising potential to be developed as a wound healing promoting agent in skin care and dermatology.

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Oligosaccharidic fractions derived from Triticum vulgare extract accelerate tissutal repairing processes in in vitro and in vivo models of skin lesions

Cited by 24 publications

References 4 publications

Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing

Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing

Drugs used in the treatment of oral mucosal diseases

<p>A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process</p>

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