Olive embryo in vitro germination potential: role of explant configuration and embryo structure among cultivars

Germanà, María Antonietta; Chiancone, Benedetta; Hammami, Sonia; Rapoport, Hava F.

doi:10.1007/s11240-014-0493-5

Cited by 8 publications

(5 citation statements)

References 15 publications

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“…Since olive endosperm is a reserve triploid tissue originated by the fecundation of the polar nuclei in the embryo sac, its analysis is equivalent to analyze embryonic tissue. The in vitro germination of excised embryos has been in depth demonstrated to rapidly break dormancy and shorten the time required to produce seedlings, speeding up olive breeding programs as well as rootstock production (Rugini et al, 1986; Acebedo et al, 1997; Troncoso et al, 2003; Germanà et al, 2014). In the present study, in vitro culture and endosperm analysis has been successfully used to initiate progeny evaluation.…”

Section: Discussionmentioning

confidence: 99%

“…All cultivars used as pollinator seem to exhibit cross-compatibility with the female parent (Coratina), since pure progenies from all the controlled crosses were obtained. The number of empty seeds, as well as other characteristics such as seed weight or stone-less seed weight, can vary considerably among olive cultivars or progenies, thus partially explaining the differences in seed germination capacity and timing (Acebedo et al, 1997; Germanà et al, 2014). The cultivar Coratina has been reported to show a good seed germination percentage by Abdallatif et al (2015).…”

Section: Discussionmentioning

confidence: 99%

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Self-Incompatibility Assessment of Some Italian Olive Genotypes (Olea europaea L.) and Cross-Derived Seedling Selection by SSR Markers on Seed Endosperms

et al. 2019

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The morphology of olive flowers allows either self- or cross-pollination that could partially explain the existence of both reproductive features in this species. However, a high degree of self-incompatibility is reported for many olive genotypes, that could be an important reproductive barrier influencing olive yield. Due to the strong environmental influence, results of compatibility tests are often contradictory, making cultivar classification quite imprecise. In this study, the self-incompatibility value has been determined for four olive genotypes (Bella di Spagna, Coratina, Leccino, and Ogliarola barese) widespread in the Mediterranean basin. Moreover, the incompatibility relationships of cultivar Coratina with some suitable pollinizers (Leccino, Oliastro, and Picholine) have been studied in controlled crosses: the in vitro germination potential of progenies has been evaluated and the selection of cross-derived embryos has been indirectly performed by the molecular characterization of the corresponding endosperm. The results increase knowledge on factors affecting self-compatibility in olive. Moreover, they provide useful information to farmers about the most effective cultivars for the set-up of new olive grove or for graft planning. Finally, they provide a new strategy and procedure based on endosperm analysis by SSRs for an accurate, fast, and relatively cheap screening of embryos/seedlings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Self-Incompatibility Assessment of Some Italian Olive Genotypes (Olea europaea L.) and Cross-Derived Seedling Selection by SSR Markers on Seed Endosperms

et al. 2019

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“…According to our results, the seed germination depends on the growth potential of the embryo. This potential depends especially on the structure of the seed surrounding the embryo (endosperm, pericarp, and glume) (Germanà et al 2014). Other factors such as hormones and environmental factors also affect the development of the embryo (Shu et al 2016).…”

Section: A Tortilismentioning

confidence: 99%

“…The study of the effects of pretreatments in the genus Acacia showed a very significant influence on the rate and mean germination times (Van der Burg et al 2014). In addition, chemical scarification by concentrated sulphuric acid gives very high germination rates compared with scarification by boiling water or sandpaper (Germanà et al 2014;Kheloufi 2017).…”

Section: A Tortilismentioning

confidence: 99%

Maximizing seed germination in five species of the genus Acacia (Fabaceae Mimosaceae)

Kheloufi

Boukhatem

Mansouri

et al. 2019

REFOR

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Seeds of many tree-species possess a hard seed coat which is impervious to water. These seeds often take a long time to germinate, resulting in heterogeneity and a delay in seedlings development which is an inconvenience for reforestation success. The aim of the present work was to determine the possibilities to improve the germination of five leguminous trees of the genus Acacia that have been recorded in the arid and the desert region of Algeria using sulphuric acid. A duration of 30 min of immersion in sulphuric acid improved the seed germination up to 97.5% and 99% for A. albida and A. laeta, respectively. Increasing the time of immersion (from 30 to 90 min) improved the germination percentages for A. ehrenbergiana and A. seyal seeds to 92.5% and 93.7%, respectively. Increasing this duration to 120 min had a positive effect on A. tortilis seed germination, improving the final germination rate up to 97%. Understanding of seed germination requirements is very important for regeneration and successful tree establishment in forest nurseries as well as for direct plantation in arid and semi-arid lands.

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“…In vitro germination of immature embryos might be helpful to shorten breeding cycles and accelerate the breeding process. In vitro cultures, plant embryos are used as an important research tool for rescuing rare hybrids (Li et al, 2014); genetic manipulation (Udomdee et al, 2014); propagation of elite and disease-free germplasm and physiological, morphological and anatomical studies (Abdolmohammadi et al, 2014;German a et al, 2014;Raomai et al, 2014). Immature embryos were also cultured successfully in vitro in several Ilex species (Hu, 1975;Sansberro et al, 2001).…”

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confidence: 99%

Immature Embryo Germination and Its Micropropagation of Ilex crenata Thunb.

Yang¹,

Zhang²,

Li³

et al. 2015

horts

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To shorten Ilex seed germination time and speed up breeding cycles, immature embryos of Ilex crenata ‘Sky Pencil’ seedlings were removed from fruits at their heart-shape stage and cultured in vitro on Murashige and Skoog (MS) medium or Woody Plant Medium (WPM) with 3% sucrose and 0.65% agar. Cultures were incubated at 27 °C for 2 weeks in darkness and subsequently moved to a growth chamber with 14-hour photoperiod (115 μmol⋅m−2⋅s–1). Embryos began to germinate 2–3 weeks after culture. The highest germination rate was 91.67% under 1/4 MS medium. Embryos cultured on MS medium also had high germination rates and produced the longest seedlings to 8.02 mm. Nodal segments with one axillary bud taken from embryo germination seedlings were cultured on MS medium with various concentrations of cytokinins and auxins for micropropagation. Zeatin (ZT; 4-hydroxy-3-methyl-trans-2-butenylaminopurine) increased the number of shoots and shoot lengths significantly more than 6-benzylaminopurine (6-BA). The recommended ZT concentration should be 2.28 µM. Rooting induction could be established on 1/4 MS medium with various concentrations of indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA). IBA at 4.14 µM produced the best rooting percentage (91.67%) and good-root quality. All rooted plantlets were transplanted into a mixture of peatmoss and perlite (1:1 v/v) and acclimatized in a mist system. The average survival rate was 88.8%. The rapid embryo germination protocol for Ilex crenata could save Ilex breeders at least 2 years compared with traditional seed germination.

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Olive embryo in vitro germination potential: role of explant configuration and embryo structure among cultivars

Cited by 8 publications

References 15 publications

Self-Incompatibility Assessment of Some Italian Olive Genotypes (Olea europaea L.) and Cross-Derived Seedling Selection by SSR Markers on Seed Endosperms

Self-Incompatibility Assessment of Some Italian Olive Genotypes (Olea europaea L.) and Cross-Derived Seedling Selection by SSR Markers on Seed Endosperms

Maximizing seed germination in five species of the genus Acacia (Fabaceae Mimosaceae)

Immature Embryo Germination and Its Micropropagation of Ilex crenata Thunb.

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