Olive (Olea europaea L.) plants transgenic for tobacco osmotin gene are less sensitive to in vitro-induced drought stress

Silvestri, Cristian; Celletti, Silvia; Cristofori, Valerio; Astolfi, Stefania; Ruggiero, Bruno; Rugini, Eddo

doi:10.1007/s11738-017-2535-1

Cited by 28 publications

(18 citation statements)

References 48 publications

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“…Plants mainly require water and nutrients for their survival, growth, and development but, due to extreme climatic changes, agriculture is usually reliant on low resource environments. It is estimated that 30% of arable land will be lost by 2021 due to drought, and by 2050 this percentage could exceed 50% [ 27 ]. In fact, drought is the major factor that contributes towards food security issues.…”

Section: Abiotic Stress Tolerancementioning

confidence: 99%

“…According to [ 30 ], in transgenic calli of rubber trees ( Heveabrasiliensis ) there was a two-fold increase in proline accumulation in response to polyethylene glycol (PEG) treatment as compared to non-transgenic calli. Similarly, in transgenic cotton ( Gossypium hirsutum ) [ 14 ] and olive ( Olea europea ) [ 27 ] plants, tobacco osmotin not only showed proline accumulation and antioxidant enzymes activity but also reduced electrolyte leakage, lipid peroxidation, and hydrogen peroxide production. In carrot ( Daucus carota ), transgenic plants have depicted a slower rate of wilting as compared to wild type plants and showed a faster recovery rate when drought stress level was increased [ 28 ].…”

Section: Abiotic Stress Tolerancementioning

confidence: 99%

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Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Bashir

Silvestri

Ahmad

et al. 2020

Plants

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Research on biologically active compounds has been increased in order to improve plant protection against various environmental stresses. Among natural sources, plants are the fundamental material for studying these bioactive compounds as their immune system consists of many peptides, proteins, and hormones. Osmotin is a multifunctional stress-responsive protein belonging to pathogenesis-related 5 (PR-5) defense-related protein family, which is involved in inducing osmo-tolerance in plants. In this scenario, the accumulation of osmotin initiates abiotic and biotic signal transductions. These proteins work as antifungal agents against a broad range of fungal species by increasing plasma membrane permeability and dissipating the membrane potential of infecting fungi. Therefore, overexpression of tobacco osmotin protein in transgenic plants protects them from different stresses by reducing reactive oxygen species (ROS) production, limiting lipid peroxidation, initiating programmed cell death (PCD), and increasing proline content and scavenging enzyme activity. Other than osmotin, its homologous proteins, osmotin-like proteins (OLPs), also have dual function in plant defense against osmotic stress and have strong antifungal activity.

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Section: Abiotic Stress Tolerancementioning

confidence: 99%

Section: Abiotic Stress Tolerancementioning

confidence: 99%

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Bashir

Silvestri

Ahmad

et al. 2020

Plants

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“…As we demonstrated that an increased level of APX prevents radical formation in transgenic plants, logically, we also found a lower amount of MDA in transgenic plants during salinity (Fig 4). Less MDA, indicating the effect of osmotin on cell membrane protection from damage by lipid peroxidation, has been already reported in transgenic olive plants exposed to drought [55].…”

Section: Resultsmentioning

confidence: 91%

Recombinant expression of osmotin in barley improves stress resistance and food safety during adverse growing conditions

et al. 2019

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Background Although many genetic manipulations of crops providing biofortified or safer food have been done, the acceptance of biotechnology crops still remains limited. We report on a transgenic barley expressing the multi-functional protein osmotin that improves plant defense under stress conditions. Methods An Agrobacterium –mediated technique was used to transform immature embryos of the spring barley cultivar Golden Promise. Transgenic barley plants of the T0 and T1 generation were evaluated by molecular methods. Transgenic barley tolerance to stress was determined by chlorophyll, total protein, malondialdehyde and ascorbate peroxidase content. Methanol extracts of i) Fusarium oxysporum infected or ii) salt-stressed plants, were characterized by their acute toxicity effect on human dermal fibroblasts (HDF), genotoxicity and affection of biodiversity interactions, which was tested through monitoring barley natural virus pathogen–host interactions–the BYDV and WDV viruses transmitted to the plants by aphids and leafhoppers. Results Transgenic plants maintained the same level of chlorophyll and protein, which significantly declined in wild-type barley under the same stressful conditions. Salt stress evoked higher ascorbate peroxidase level and correspondingly less malondialdehyde. Osmotin expressing barley extracts exhibited a lower cytotoxicity effect of statistical significance than that of wild-type plants under both types of stress tested on human dermal fibroblasts. Extract of Fusarium oxysporum infected transgenic barley was not able to damage DNA in the Comet assay, which is in opposite to control plants. Moreover, this particular barley did not affect the local biodiversity. Conclusion Our findings provide a new perspective that could help to evaluate the safety of products from genetically modified crops.

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“…In the case of soybean, the GmOLPb gene was highly induced in leaves but lower in the stem by methyl jasmonate stimulation [ 38 ]. Previous studies showed that osmotin has the ability to protect chlorophyll and photosynthetic machinery, prevent reactive oxygen species accumulation and stimulate more proline accumulation [ 5 , 16 , 55 ]. The amounts of KoOsmotin expression were all induced to a small extent at first 7 d, and the expression levels in leaves were higher than in stems and roots.…”

Section: Discussionmentioning

confidence: 99%

“…Although further investigations are needed, we can infere that KoOsmotin might also possess cold-resistance in transgenic plants. Besides, many scientists have successfully transformed osmotin gene to induce salt, drought and osmotic stress resistance in transgenic plants [ 16 , 19 , 26 , 55 , 57 , 58 ], as well as antifungal activity [ 40 , 59 , 60 ]. These literatures lead us to the conclusion that osmotin is an important PR-protein and is expected to be successfully used in developing plant defense mechanisms in the future.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress

et al. 2021

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Background Low temperature is a major abiotic stress that seriously limits mangrove productivity and distribution. Kandelia obovata is the most cold-resistance specie in mangrove plants, but little is known about the molecular mechanism underlying its resistance to cold. Osmotin is a key protein associated with abiotic and biotic stress response in plants but no information about this gene in K. obovata was reported. Results In this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal signal peptide, which were common signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) model of KoOsmotin, contained one α-helix and eleven β-strands, was formed by three characteristic domains. Database comparisons of the KoOsmotin showed the closest identity (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree also revealed that the KoOsmotin was clustered in the branch of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to both the plasma membrane and cytoplasm by the subcellular localization analysis. Gene expression showed that the KoOsmotin was induced primarily and highly in the leaves of K. obovata, but less abundantly in stems and roots. The overexpressing of KoOsmotin conferred cold tolerance in Escherichia coli cells. Conclusion As we known, this is the first study to explore the osmotin of K. obovata. Our study provided valuable clues for further exploring the function of KoOsmotin response to stress.

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Olive (Olea europaea L.) plants transgenic for tobacco osmotin gene are less sensitive to in vitro-induced drought stress

Cited by 28 publications

References 48 publications

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Recombinant expression of osmotin in barley improves stress resistance and food safety during adverse growing conditions

Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress

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