OM‐LV20, a novel peptide from odorous frog skin, accelerates wound healing in vitro and in vivo

Li, Xiaojie; Wang, Ying; Zou, Zhirong; Yang, Meifeng; Wu, Cheng-Shong; Su, Yunshan; Tang, Jing

doi:10.1111/cbdd.13063

Cited by 57 publications

(53 citation statements)

References 34 publications

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“…At this time, the promotion of wound healing appears to be the most promising application for frog skin peptides because of their abilities to prevent microbial invasion, reduce inflammation and induce migration of keratinocytes [ 70 ]. Several candidates have shown therapeutic relevance [ 28 , 71 , 72 ]. Obstacles to such applications include the cytotoxicity of many frog skin peptides for healthy human cells, as well as their short half-lives in circulation.…”

Section: Discussionmentioning

confidence: 99%

The Potential of Frog Skin-Derived Peptides for Development into Therapeutically-Valuable Immunomodulatory Agents

et al. 2017

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The aim of this article is to review the immunoregulatory actions of frog skin-derived peptides in order to assess their potential as candidates for immunomodulatory or anti-inflammatory therapy. Frog skin peptides with demonstrable immunomodulatory properties have been isolated from skin secretions of a range of species belonging to the families Alytidae, Ascaphidae, Discoglossidae, Leptodactylidae, Pipidae and Ranidae. Their effects upon production of inflammatory and immunoregulatory cytokines by target cells have been evaluated ex vivo and effects upon cytokine expression and immune cell activity have been studied in vivo by flow cytometry after injection into mice. The naturally-occurring peptides and/or their synthetic analogues show complex and variable actions on the production of proinflammatory (TNF-α, IL-1β, IL-12, IL-23, IL-8, IFN-γ and IL-17), pleiotropic (IL-4 and IL-6) and immunosuppressive (IL-10 and TGF-β) cytokines by peripheral and spleen cells, peritoneal cells and/or isolated macrophages. The effects of frenatin 2.1S include enhancement of the activation state and homing capacity of Th1-type lymphocytes and NK cells in the mouse peritoneal cavity, as well as the promotion of their tumoricidal capacities. Overall, the diverse effects of frog skin-derived peptides on the immune system indicate their potential for development into therapeutic agents.

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Section: Discussionmentioning

confidence: 99%

The Potential of Frog Skin-Derived Peptides for Development into Therapeutically-Valuable Immunomodulatory Agents

et al. 2017

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“…Cellular wound healing was determined according to earlier research [ 19 ]. In short, HaCaT cells and human skin fibroblasts (HSFs) were cultured in DMEM/F12 medium (BI, Israel) with 10% FBS (BI, Israel), 100 units/ml of streptomycin and 100 units/ml of penicillin, and incubated in a humidified atmosphere of 5% CO 2 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds

Bian

Meng

et al. 2018

Bioscience Reports

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Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and financially. Therefore, it is urgent to develop new drugs to accelerate the healing of wounds. Bioactive peptides, which are relatively low cost, easy to produce, store and transport, have become an excellent choice. In this research, we identified a novel peptide OA-GL21, with an amino acid sequence of ‘GLLSGHYGRVVSTQSGHYGRG’, from the skin secretions of Odorrana andersonii. Our results showed that OA-GL21 exerted the ability to promote wound healing of human keratinocytes (HaCaT) and human fibroblasts in a dose- and time-denpendent manner. However, OA-GL21 had no significant effect on the proliferation of these two cells. Significantly, OA-GL21 showed obvious ability to promote wound healing in the full-thickness skin wound model in dose- and scar-free manners. Further studies showed that OA-GL21 had no direct antibacterial, hemolytic, and acute toxic activity; it had weak antioxidant activities but high stability. In conclusion, this research proved the promoting effects of OA-GL21 on cellular and animal wounds, and thus provided a new peptide template for the development of wound-repairing drugs.

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“…Peptide fingerprinting of 21 characteristic peptides in APs was performed using liquid chromatography-mass spectrometry (LC-MS/MS). The molecular weights of APs were in the range 1512.83 Da to 2241.05 Da (amino acid residue [10][11][12][13][14][15][16][17][18][19][20][21]. Two peptide fragments of Gln-Leu and Asp-Leu recurred in the 21 characteristic peptide sequences.…”

Section: Basic Composition Analysis Of Apsmentioning

confidence: 99%

“…However, pharmaceutical companies face major challenges in drug production, cost, research and development [12]. Despite the challenges, marine bioactive peptides, especially small peptides with high activity, specificity and stability have continued to attract research attention compared with drugs with higher cost, less safety and therapeutic delivery problems [13]. Marine-derived active peptides have functionality in wound healing, such as the effect of orally administered Alaska Pollock low molecular weight peptide on skin wound healing [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

et al. 2019

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Skin wound healing, especially chronic wound healing, is a common challenging clinical problem. It is urgent to broaden the sources of bioactive substances that can safely and efficiently promote skin wound healing. This study aimed to observe the effects of active peptides (APs) of the mantle of Pinctada martensii on wound healing. After physicochemical analysis of amino acids and mass spectrometry of APs, the effect of APs on promoting healing was studied through a whole cortex wound model on the back of mice for 18 consecutive days. The results showed that APs consisted of polypeptides with molecular weights in the range 302.17–2936.43 Da. The content of polypeptides containing 2–15 amino acids accounted for 73.87%, and the hydrophobic amino acids accounted for 56.51%. Results of in vitro experimentation showed that mice in APs-L group which were fed a low dose of APs (0.5 g/kg bw) had a shortened epithelialization time due to a shortening inflammatory period (p < 0.05). Mechanistically, this relied on its specific ability to promote the proliferation of CD31, FGF and EGF which accelerated the percentage of wound closure. Moreover, the APs-L group mice had enhanced collagen synthesis and increased type III collagen content in their wounds through a TGF-β/Smad signaling pathway (p > 0.05). Consequently, scar formation was inhibited and wound healing efficiency was significantly improved. These results show that the APs of Pinctada martensii promote dermal wound healing in mice and have tremendous potential for development and utilization in skin wound healing.

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OM‐LV20, a novel peptide from odorous frog skin, accelerates wound healing in vitro and in vivo

Cited by 57 publications

References 34 publications

The Potential of Frog Skin-Derived Peptides for Development into Therapeutically-Valuable Immunomodulatory Agents

The Potential of Frog Skin-Derived Peptides for Development into Therapeutically-Valuable Immunomodulatory Agents

OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds

Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

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