The development of imaging, diagnosis, prognosis and early detection of diseases has been greatly impacted by nanotechnology by enhancing already existing clinically applicable technologies. With the help of their capacity to alter nanoparticles for molecular‐level specificity, tissue‐specific diagnosis is made possible gratitude to the unique biophysical features of the nanoparticles that enable contrast augmentation will boost biomedical imaging. The unique prospect of multiplexing is possible by the fact that minute changes in the nanoparticles’ size or composition can have significant effects on their optical, magnetic, or electrical capabilities. This article examines nanotechnology's function in biomedical imaging. In this article, the fundamentals and applications of biomedical imaging, pharmaceutical application of microbial surfactants, intelligent drug delivery systems, and green metallic nanoparticle manufacturing are all examined. The biomedical applications comprising organic (carbon nanotubes, metal oxide and liposomes) and inorganic (metal oxide, metal) nanoparticles, and materials with nanopatterns in diagnostics, biosensing, and bioimaging devices, along with drug delivery systems, are the main topics of discussion. studies conducted in vitro and in vivo have demonstrated that different nanoparticles can be utilized to detect seizures early and precisely along with to treat them successfully. To decrease the possible toxicity and enable improved target specificity, respectively, more development in the synthesis and functionalization of adaptable nanotechnologies is required.