Omega-3 fatty acid desaturase (FAD3, FAD7, FAD8) gene expression and linolenic acid content in cowpea leaves submitted to drought and after rehydration

Torres-Franklin, Maria-Lucia; Repellin, Anne; Huynh, Van-Biet; d’Arcy-Lameta, Agnès; Zuily-Fodil, Yasmine; Pham-Thi, Anh-Thu

doi:10.1016/j.envexpbot.2008.12.010

Cited by 68 publications

(32 citation statements)

References 34 publications

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“…Salinity also could motivate the up-regulation of CiFAD3 expression (Zhang et al 2010). The leaves of cowpea increased expression of VuFAD3 in response to mild drought stress (Torres-Franklin et al 2009). In the present study, three fatty acid desaturase genes were differently regulated by high temperature at 42°C, salt stress with 0.15 mM NaCl and drought simulated by 15% PEG.…”

Section: Resultsmentioning

confidence: 99%

Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.)

Zhang

Wang

et al. 2010

Biotechnol Lett

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The critical chilling temperature of cold-sensitive lima bean (Phaseolus lunatus L.) seedlings was determined to be approx. 8°C. The full-length cDNAs of PlSAD, PlFAD2, and PlFAD3 encoding three fatty acid desaturases were isolated from lima bean leaves. The PlSAD open reading frame (ORF) had 1,317 bp and a single intron of 601 bp, encoding a polypeptide of 438 amino acids that showing 86% homology with the plastidial stearoyl-acyl carrier protein desaturase in soybean. The PlFAD2 ORF contained 1,059 bp and was uninterrupted, encoding a polypeptide of 352 amino acids having 85% identity with the microsomal omega-6 desaturase GmFAD2-3 in soybean. PlFAD3 ORF had 1,116 bp and seven introns, encoding a polypeptide of 371 amino acids showing 91% homology with the microsomal omega-3 desaturase FAD3 in cowpea. PlSAD and PlFAD2 were expressed highly in leaves but poorly in roots and stems, while PlFAD3 was expressed in three tissues. All three genes were significantly induced in leaves by drought. PlSAD and PlFAD3 in leaves were down-regulated by high temperature and salinity, whereas PlFAD2 was up-regulated by the two stresses. The expression patterns of the three genes in lima bean leaves under suboptimal temperature, 18°C, were different from those under 8°C. The down-regulation of PlSAD transcript at chilling temperature might be an important factor contributing to chilling susceptibility for lima bean plants.

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Section: Resultsmentioning

confidence: 99%

Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.)

Zhang

Wang

et al. 2010

Biotechnol Lett

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“…5). Cell membrane lipids are strongly influenced by environmental stresses, including flooding, and have been shown to be comprised of high levels of polyunsaturated fatty acids through the fatty acid desaturation activity of SAD under such conditions [61]. The biosynthesis of polyunsaturated fatty acids is initiated by the introduction of a double bond into stearic acid by SAD.…”

Section: Validation Of Differentially Identified Proteinsmentioning

confidence: 99%

Gel-free quantitative proteomic approach to identify cotyledon proteins in soybean under flooding stress

Kamal

Hamid

Sakata

et al. 2015

Journal of Proteomics

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“…Evidence has been accumulating that trienoic fatty acids of thylakoid membrane lipids are required to maintain photosynthetic competence, particularly at low temperature (McConn and Browse, 1998;Routaboul et al, 2000;Vijayan and Browse, 2002;Barkan et al, 2006). In addition, membrane polyunsaturation has been shown to be correlated with drought tolerance as well as with cold and freezing tolerance (Im et al, 2002;Zhang et al, 2005;Mène-Saffrané et al, 2009;Torres-Franklin et al, 2009). Reduction of 18:3 fatty acid in chloroplast membrane by silencing a plastidial v-3 desaturase (FAD7) in tobacco plants causes a decrease in drought/salt tolerance (Im et al, (340293, 340318, 340367, 340378, and 340397), the snrk2.6 mutant, and its null sibling (Null).…”

Section: Snrk26 Overexpression Increased Leaf Fatty Acid Desaturationmentioning

confidence: 99%

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis

et al. 2010

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In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeostasis, the functional significance of the two plant-specific subfamilies SnRK2 and SnRK3 in these critical processes is poorly understood. We show here that SnRK2.6, previously identified as crucial in the control of stomatal aperture by abscisic acid (ABA), has a broad expression pattern and participates in the regulation of plant primary metabolism. Inactivation of this gene reduced oil synthesis in Arabidopsis (Arabidopsis thaliana) seeds, whereas its overexpression increased Suc synthesis and fatty acid desaturation in the leaves. Notably, the metabolic alterations in the SnRK2.6 overexpressors were accompanied by amelioration of those physiological processes that require high levels of carbon and energy input, such as plant growth and seed production. However, the mechanisms underlying these functionalities could not be solely attributed to the role of SnRK2.6 as a positive regulator of ABA signaling, although we demonstrate that this kinase confers ABA hypersensitivity during seedling growth. Collectively, our results suggest that SnRK2.6 mediates hormonal and metabolic regulation of plant growth and development and that, besides the SnRK1 kinases, SnRK2.6 is also implicated in the regulation of metabolic homeostasis in plants.Plants are constantly confronted by biotic and abiotic stresses and nutrient deprivation that disrupt metabolic and energy homeostasis or diminish carbon and energy availability for maintaining cell vitality, growth, and proliferation. It is believed that maintaining energy balance and availability at the cellular and organism levels is critical for optimizing plant growth and development. This underscores the cellular and physiological importance of energy sensors that control energy balance through regulating fundamental metabolic pathways in response to nutritional and environmental stresses.At present, a prevailing view is that energy sensors are evolutionarily conserved in eukaryotes, which are represented by Snf1 (for sucrose nonfermenting-1) in yeast, AMPK (for AMP-activated protein kinase) in

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Omega-3 fatty acid desaturase (FAD3, FAD7, FAD8) gene expression and linolenic acid content in cowpea leaves submitted to drought and after rehydration

Cited by 68 publications

References 34 publications

Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.)

Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.)

Gel-free quantitative proteomic approach to identify cotyledon proteins in soybean under flooding stress

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis

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