Omega-3 Fatty Acid Lipid Emulsion Reduces LPS-Stimulated Macrophage TNF-<i>α</i> Production

Babcock, Tricia A.; Helton, W. Scott; Hong, David S.; Espat, N. Joseph

doi:10.1089/109629602760105817

Cited by 68 publications

(54 citation statements)

References 11 publications

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“…The results from the present study showing increased TNF-a secretion by RAW 264.7 macrophages incubated with omega-3 PUFA compared with that when incubated without fatty acids or with omega-6 PUFA are in disagreement with results from previous studies [2,22,25]. In these studies, RAW 264.7 macrophages incubated with EPA or with Omegaven, a fatty acid emulsion consisting of various fatty acids, including EPA and DHA, secreted less TNF-a than cells incubated without EPA [22] or with Lipovenos, an omega-6 PUFA emulsion [25], respectively.…”

Section: Discussioncontrasting

confidence: 99%

The Effects of Omega‐3 Polyunsaturated Fatty Acids on TNF‐α and IL‐10 Secretion by Murine Peritoneal Cells In Vitro

Skuladottir

Petursdottir

Harðardóttir

2007

Lipids

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Omega-3 polyunsaturated fatty acids (PUFA) affect immune response, partly by affecting cytokine secretion. Omega-3 PUFA decrease tumor necrosis factor (TNF)-alpha secretion by RAW 264.7 macrophages but increase TNF-alpha secretion by primary elicited peritoneal macrophages in vitro. In this study, the effects of omega-3 and omega-6 PUFA on lipopolysaccharide induced TNF-alpha and interleukin (IL)-10 secretion by murine primary resident and elicited peritoneal macrophages and by RAW 264.7 macrophages, were examined in vitro using an enzyme-linked immunosorbent assay. In addition, the effects of dietary omega-3 PUFA on the number of cells secreting these cytokines were examined with enzyme-linked immunospot assay. All cell types secreted more TNF-alpha but similar amounts of IL-10 when incubated with the omega-3 PUFA, eicosapentaenoic acid or docosahexaenoic acid, compared with that when incubated with the omega-6 PUFA, linoleic acid or arachidonic acid. Dietary fish oil did not affect the number of TNF-alpha secreting resident peritoneal macrophages but decreased the number of macrophages secreting IL-10 ex vivo. These results show that dietary omega-3 PUFA and omega-3 PUFA added to cells in vitro increase TNF-alpha secretion by resident peritoneal macrophages, probably by a direct effect on the cells. In contrast, omega-3 PUFA did not affect IL-10 secretion by the cells but decreased the number of cells secreting IL-10 ex vivo, possibly by affecting cell recruitment, maturation or proliferation.

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Section: Discussioncontrasting

confidence: 99%

The Effects of Omega‐3 Polyunsaturated Fatty Acids on TNF‐α and IL‐10 Secretion by Murine Peritoneal Cells In Vitro

Skuladottir

Petursdottir

Harðardóttir

2007

Lipids

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“…Equivalent FA concentrations for the ω-3 and ω-6 emulsions were prepared by dilution for experiments as previously and optimal concentrations for macrophage TNF-α inhibition were previously determined by dose-response studies. 8,19 Briefly, the ω-3 FA emulsion contains 2.1g/100 ml EPA was added in concentrations of 1.44 ul · ml −1 corresponding to 100 µM EPA. Control experiments were carried out with a lipid emulsion based on soybean oil (ω-6 FA) at the same dilutions to exclude a non-specific effect of lipid emulsion on the cells and cell viability assays were also performed and found no increase in toxicity with these emulsions at these concentrations (4).…”

Section: Experimental Designmentioning

confidence: 99%

Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Inducible Nitric Oxide Synthase and Nitric Oxide Production Is Decreased by an Omega-3 Fatty Acid Lipid Emulsion

Aldridge

Razzak

Babcock

et al. 2008

Journal of Surgical Research

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Background-Omega-3 FA (ω-3 FA) have been demonstrated to have anti-inflammatory properties; postulated to occur through several principal mechanisms, including 1) displacement of arachidonic acid from the cellular membrane, 2) shifting of PGE 2 and LTB 4 production and 3) molecular level alterations including decreased activation of NF-κB and AP-1. An additional regulator that is likely associated is the production of nitric oxide (NO) by nitric oxide synthetase. NO is a short-lived free radical involved in many biological functions. However, excessive NO production can lead to complications, suggesting that decreased NO production is a potential target for some inflammatory diseases. We hypothesized that pretreating with an ω-3 FA lipid emulsion would decrease the production of NO in macrophages and that this effect would occur through alterations in inducible nitric oxide synthetase (iNOS).

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“…Finally, both EPA and DHA give rise to a newly discovered family of anti-inflammatory mediators called resolvins (Serhan et al, 2002). Most of these activities directly target monocyte/macrophage defensive functions by modulating phagocytosis (Halvorsen et al, 1997), chemotaxis (Schmidt et al, 1992) and proinflammatory cytokine expression (Babcock et al, 2002). While still not completely understood, the main biological effect of x3 long chains fatty acids has been ascribed to their incorporation in cell membrane phospholipids, thus affecting eicosanoid synthesis and altering membrane fluidity (Das, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

Lecchi

Invernizzi

Agazzi

et al. 2013

Research in Veterinary Science

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a b s t r a c tThe present study investigated the in vitro effects on caprine monocytes of two x-3 PUFAs, namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on lipid droplet formation, an emerging process of fundamental importance in innate immunity regulation. The mRNA abundance of PAT protein family (PLIN1, PLIN2 and PLIN3), involved in the formation and trafficking of the droplets, was also assessed. The effects of EPA and DHA on monocyte apoptosis were studied as well. The number of lipid droplets per cell was found to be dependent on both type and concentration of fatty acid. x-3 PUFAs upregulated PLIN3 and PLIN2 gene expression, as well as apoptosis rate. The present findings suggest that PUFA might modify innate immune functions of goat monocytes by interfering with the formation of lipid droplets and by upregulating proteins belonging to PAT protein family.

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Omega-3 Fatty Acid Lipid Emulsion Reduces LPS-Stimulated Macrophage TNF-α Production

Cited by 68 publications

References 11 publications

The Effects of Omega‐3 Polyunsaturated Fatty Acids on TNF‐α and IL‐10 Secretion by Murine Peritoneal Cells In Vitro

The Effects of Omega‐3 Polyunsaturated Fatty Acids on TNF‐α and IL‐10 Secretion by Murine Peritoneal Cells In Vitro

Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Inducible Nitric Oxide Synthase and Nitric Oxide Production Is Decreased by an Omega-3 Fatty Acid Lipid Emulsion

Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

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