Omegaven Improves Skin Morphometric Indices in Diabetic Rat Model Wound Healing

Abstract

The global prevalence of diabetes is estimated to be 200 million people, and it is likely to increase to 333 million people by 2025. Different cells, are known to participate in three main phases of wound healing. Omega-3 fatty acids influence cytokines and growth factors which affects the presence of inflammatory cells in wound area as well, but how this event specifically influences the role of fibroblasts, macrophages and angiogenesis in wound healing is not obviously understood. In this experimental study seventy male Wistar rats after induction of diabetes type-one by streptozotocin (STZ) (55 mg/kg) were divided into two groups, Experimental group receiving omegaven intraperitoneally and control group which underwent the injection of mineral oil. Streptozotocin was used for the induction of diabetes type 1. Diabetic male wistar rats were scarified at 1, 3, 5, 7 and 15 days after the excision was made. To estimate orphometric indices, histological sections were provided by stereological methods. It was found that wound area significantly decreased on day 7 in experimental group by omega-3 fatty acids. The number of fibroblasts increased significantly on days 5 and 7 in the experimental group. The number of neovascular significantly decreased on day 7 in the experimental group. This study implied that it seems omegaven is able to improve morphometric indices during wound healing and make healing faster.

Exploring Skin Wound Healing Models and the Impact of Natural Lipids on the Healing Process

“…Intraperitoneal administration of a fish-oil emulsion rich in EPA and DHA improved excisional skin wound healing in diabetic male rats [102].…”

“…Additionally, DHA enhanced TGFβ expression and the keratinocyte differentiation marker, involucrin, promoting tissue repair. In a separate investigation, diabetic male Wistar rats induced by streptozotocin underwent excisional wounds on their dorsal skin and received an intraperitoneal administration of Omegaven, a fish-oil emulsion containing EPA and DHA [102]. This intervention resulted in faster wound healing and improved skin morphometric indices.…”

Metabolic reprogramming in skin wound healing

“…In diabetic rat skin wound healing models, omega-3 PUFAs have been found to alter proinflammatory cytokine production, which substantially decreased the number of grade three mast cells on days 3 and 5 and the wound area by day 7 [ 61 ]. The number of fibroblasts capable of repair was significantly increased in the wound areas of omega-3 PUFA-treated rats, whereas that of macrophages was decreased, thereby accelerating healing [ 62 ].…”

Efeitos Da Microcorrentes Na Cicatrização De Feridas Em Ratos Diabéticos

“…O software Excel 2010 foi utilizado para entrada dos dados e confecção das tabelas. Além disso, estudos demonstram que o diabetes mellitus causa efeitos deletérios para o processo de cicatrização, tendo em vista que essa patologia gera redução na organização do colágeno e do suprimento sanguíneo, aumento da viscosidade do sangue e atrasos na infiltração de células inflamatórias (13,14) .…”