Omni-directional actuation of magnetically driven microtool for enucleation of oocyte

Arai, Fumihito; Inomata, Naoki; Kudo, Shogo; Yamanishi, Yoko; Mizunuma, Takehito

doi:10.1109/memsys.2010.5442419

Cited by 8 publications

(2 citation statements)

References 10 publications

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“…For instance, a method featuring a magnetically-driven microtool (MMT) was robotically controlled in a microfluidic chip by using permanent magnets and used for nucleus isolation. 14,15 Similarly, a robotic, microfluidic device for extraction of nuclei from oocytes was reported. 16 However, the yield of viable nuclei was relatively low, and the operation time was lengthy.…”

Section: Introductionmentioning

confidence: 99%

Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform

2016

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The extraction of a cell's nucleus is an essential technique required for a number of procedures, such as disease diagnosis, genetic replication, and animal cloning. However, existing nucleus extraction techniques are relatively inefficient and labor-intensive. Therefore, this study presents an innovative, microfluidics-based approach featuring optically-induced cell lysis (OICL) for nucleus extraction and collection in an automatic format. In comparison to previous micro-devices designed for nucleus extraction, the new OICL device designed herein is superior in terms of flexibility, selectivity, and efficiency. To facilitate this OICL module for continuous nucleus extraction, we further integrated an optically-induced dielectrophoresis (ODEP) module with the OICL device within the microfluidic chip. This on-chip integration circumvents the need for highly trained personnel and expensive, cumbersome equipment. Specifically, this microfluidic system automates four steps by 1) automatically focusing and transporting cells, 2) releasing the nuclei on the OICL module, 3) isolating the nuclei on the ODEP module, and 4) collecting the nuclei in the outlet chamber. The efficiency of cell membrane lysis and the ODEP nucleus separation was measured to be 78.04 ± 5.70% and 80.90 ± 5.98%, respectively, leading to an overall nucleus extraction efficiency of 58.21 ± 2.21%. These results demonstrate that this microfluidics-based system can successfully perform nucleus extraction, and the integrated platform is therefore promising in cell fusion technology with the goal of achieving genetic replication, or even animal cloning, in the near future.

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Section: Introductionmentioning

confidence: 99%

Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform

2016

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“…The MMT driven by a permanent magnet can be applied to wide range of cell manipulations such as loader, sorter, droplet generation, etc. [9][10][11].Enucleation by MMT was conducted previously; MMT driven by the HPD with ultrasonic vibration applied to the microchip could obtain 1.1 μm in position accuracy, and also by fabricated riblet type of MMT, which could achieve high performance in speed [12][13][14]. However it is difficult to handle the oocyte to control its posture and remove only the nucleus part since the oocyte is operated in an unconfined space.…”

Section: Introductionmentioning

confidence: 99%

Continuous enucleation of bovine oocyte by microrobot with local flow distribution control

Feng

Ichikawa

Arai

et al. 2012

2012 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3m-Nano)

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We present a continuous enucleation process of bovine oocytes on a microfluidic chip to achieve the continuous cutting of the oocytes and increasing the potential viability of the enucleated oocyte. By combining microfluidic chip and micororobotics, the flow in a channel can be actively controlled and we achieved successive operations of 1) loading oocyte, 2) control cutting volume and 3) removing nucleus. The magnetically actuated microrobot can control local fluid flow by changing its position like gate valve and control fluid force distributions in a microchip which govern oocyte movement in a chip. The optimally designed microchannel for enucleation enables continuous operation and cutting bovine oocyte with smooth manner by hydrodynamic force. The new system is propose here to show advantages by the means of the continuous operation on oocytes in a short term, cutting precision and great potentiality on continuous enucleation process for clone technology.

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Robotic orientation control and enucleation of cells

Feng¹,

Zhang²,

Gan³

et al. 2023

Robotics for Cell Manipulation and Characterization

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Omni-directional actuation of magnetically driven microtool for enucleation of oocyte

Cited by 8 publications

References 10 publications

Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform

Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform

Continuous enucleation of bovine oocyte by microrobot with local flow distribution control

Robotic orientation control and enucleation of cells

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