OmpR, a Central Integrator of Several Cellular Responses in Yersinia enterocolitica

Brzostek, Katarzyna; Skorek, Karolina; Raczkowska, Adrianna

doi:10.1007/978-1-4614-3561-7_40

Cited by 22 publications

(20 citation statements)

References 50 publications

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“…In the reports of the European Food Safety Authority (EFSA), Y. enterocolitica is listed as the third most common enteropathogen after Campylobacter and Salmonella (Zadernowska et al, 2014). Y. enterocolitica is widely distributed in the environment and can be found in soil, water, animals and various food products (Bari et al, 2011;Rahman et al, 2011), where constant changes in physicochemical conditions, including osmolarity, pH, temperature, light intensity, medium viscosity, and nutrient availability often threaten bacterial survival (Bottone, 1997;Brzostek et al, 2012). These challenges are met by rapid adaptation of the pathogen to varying growth conditions provided by two-component regulatory systems (TCSs), also called phosphorelays, as they sense the extracellular signals and perform cascade phosphorylation in response, thus regulating the expression of genes related to flagellar synthesis, biofilm formation, and virulence (Clarke, 2010;Clarke and Voigt, 2011;Brzostek et al, 2012;Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Osmoregulated Periplasmic Glucans Transmit External Signals Through Rcs Phosphorelay Pathway in Yersinia enterocolitica

Meng

Huang

et al. 2020

Front. Microbiol.

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Fast response to environmental changes plays a key role in the transmission and pathogenesis of Yersinia enterocolitica. Osmoregulated periplasmic glucans (OPGs) are known to be involved in environmental perception of several Enterobacteriaceae pathogens; however, the biological function of OPGs in Y. enterocolitica is still unclear. In this study, we investigated the role of OPGs in Y. enterocolitica by deleting the opgGH operon encoding enzymes responsible for OPGs biosynthesis. Complete loss of OPGs in the opgGH mutant resulted in decreased motility, c-di-GMP production, biofilm formation and smaller cell size, whereas the overproduction of OPGs through restoration of opgGH expression promoted c-di-GMP/biofilm production and increased antibiotic resistance of Y. enterocolitica. Gene expression analysis revealed that opgGH deletion reduced transcription of flhDC, ftsAZ, hmsT and hmsHFRS genes regulated by the Rcs phosphorelay system, whereas additional deletion of rcs family genes (rcsF, rcsC, or rcsB) reversed this effect and restored motility and c-di-GMP/biofilm production but further reduced cell size. Furthermore, disruption of the Rcs phosphorelay increased the motility and promoted the induction of biofilm and c-di-GMP production regulated by OPGs through upregulating the expression of flhDC, hmsHFRS, and hmsT. However, deletion of genes encoding the EnvZ/OmpR phosphorelay downregulated the flhDC, hmsHFRS and hmsT expression, leading to the decreased motility and prevented the induction of biofilm and c-di-GMP production regulated by OPGs. These results indicated that Rcs phosphorelay had the effect on OPGs-mediated functional responses in Y. enterocolitica. Our findings disclose part of the biological role of OPGs and the underlying molecular mechanisms associated with Rcs system in the regulation of the pathogenic phenotype in Y. enterocolitica.

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Section: Introductionmentioning

confidence: 99%

Osmoregulated Periplasmic Glucans Transmit External Signals Through Rcs Phosphorelay Pathway in Yersinia enterocolitica

Meng

Huang

et al. 2020

Front. Microbiol.

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“…), and biofilm (Brzostek et al . ). The five virulence genes cover early and late stages of infection and include chromosomal and plasmid‐encoded genes.…”

Section: Resultsmentioning

confidence: 97%

Acetoacetate and ethyl acetoacetate as novel inhibitors of bacterial biofilm

Horne

Schroeder

Murphy

et al. 2018

Lett Appl Microbiol

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Bacterial biofilms are communities of bacteria that form on surfaces and are extremely difficult to remove by conventional physical or chemical techniques, antibiotics or the human immune system. Despite advanced technologies, biofilm still contributes to 60 to 80% of human bacterial infections (NIH and CDC) and cause problems in many natural, environmental, bioindustrial or food processing settings. The discovery of novel substances that inhibit biofilm without increasing the virulence of the bacteria opens doors for countless applications where a reduction of biofilm is desired.

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“…There is now a considerable body of evidence indicating that OmpR is involved in the regulation of fundamental physiological properties in Yersinia species (Gao et al, 2011;Gueguen et al, 2013;Reboul et al, 2014;Bontemps-Gallo et al, 2019). Our research on Y. enterocolitica strain Ye9N (bio-serotype 2/O:9) suggests that OmpR performs a variety of functions, some of which differ from those described in other bacteria (Brzostek et al, 2012). We have shown that OmpR plays a regulatory function in modulating the abundance of outer membrane proteins, including virulence factors like invasin Inv and adhesin YadA (Brzostek et al, 2007;Skorek et al, 2013;Nieckarz et al, 2016).…”

Section: Introductionmentioning

confidence: 84%

Urease Expression in Pathogenic Yersinia enterocolitica Strains of Bio-Serotypes 2/O:9 and 1B/O:8 Is Differentially Regulated by the OmpR Regulator

et al. 2020

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Yersinia enterocolitica exhibits a dual lifestyle, existing as both a saprophyte and a pathogen colonizing different niches within a host organism. OmpR has been recognized as a regulator that controls the expression of genes involved in many different cellular processes and the virulence of pathogenic bacteria. Here, we have examined the influence of OmpR and varying temperature (26 • C vs. 37 • C) on the cytoplasmic proteome of Y. enterocolitica Ye9N (bio-serotype 2/O:9, low pathogenicity). Differential label-free quantitative proteomic analysis indicated that OmpR affects the cellular abundance of a number of proteins including subunits of urease, an enzyme that plays a significant role in acid tolerance and the pathogenicity of Y. enterocolitica. The impact of OmpR on the expression of urease under different growth conditions was studied in more detail by comparing urease activity and the transcription of ure genes in Y. enterocolitica strains Ye9N and Ye8N (highly pathogenic bio-serotype 1B/O:8). Urease expression was higher in strain Ye9N than in Ye8N and in cells grown at 26 • C compared to 37 • C. However, low pH, high osmolarity and the presence of urea did not have a clear effect on urease expression in either strain. Further analysis showed that OmpR participates in the positive regulation of three transcriptional units encoding the multi-subunit urease (ureABC, ureEF, and ureGD) in strain Ye9N, but this was not the case in strain Ye8N. Binding of OmpR to the ureABC and ureEF promoter regions was confirmed using an electrophoretic mobility shift assay, suggesting that this factor plays a direct role in regulating the transcription of these operons. In addition, we determined that OmpR modulates the expression of a ureR-like gene encoding a putative regulator of the ure gene cluster, but in the opposite manner, i.e., positively in Ye9N and negatively in Ye8N. These findings provide some novel insights into the function of OmpR in adaptation strategies of Y. enterocolitica.

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OmpR, a Central Integrator of Several Cellular Responses in Yersinia enterocolitica

Cited by 22 publications

References 50 publications

Osmoregulated Periplasmic Glucans Transmit External Signals Through Rcs Phosphorelay Pathway in Yersinia enterocolitica

Osmoregulated Periplasmic Glucans Transmit External Signals Through Rcs Phosphorelay Pathway in Yersinia enterocolitica

Acetoacetate and ethyl acetoacetate as novel inhibitors of bacterial biofilm

Urease Expression in Pathogenic Yersinia enterocolitica Strains of Bio-Serotypes 2/O:9 and 1B/O:8 Is Differentially Regulated by the OmpR Regulator

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