OmpR, a response regulator of the two-component signal transduction pathway, influences inv gene expression in Yersinia enterocolitica O9

Abstract

The environmental control of invasin (inv) expression in Yersinia enterocolitica is mediated by a regulatory network composed of negative and positive regulators of inv gene transcription. Previously, we demonstrated that OmpR, a response regulator of the two-component signal transduction pathway EnvZ/OmpR, negatively regulates inv gene expression in Y. enterocolitica O9 by direct interaction with the inv promoter region. This study was undertaken to clarify the role of OmpR in the inv regulatory circuit in which RovA protein has been shown to positively regulate inv transcription. Using ompR, rovA, and ompR rovA Y. enterocolitica mutant backgrounds we showed that the inhibitory effect of OmpR on inv transcription may be observed only when RovA is present/active in Y. enterocolitica cells. To extend our research on inv regulation we examined the effect of OmpR on rovA gene expression. Analysis of rovA-lacZ transcriptional fusion in Y. enterocolitica wild-type and ompR background indicated that OmpR does not influence rovA expression. Thus, our results indicate that OmpR influences inv expression directly via binding to the inv promoter, but not through modulation of rovA expression.

Expression of the AcrAB Components of the AcrAB-TolC Multidrug Efflux Pump of Yersinia enterocolitica Is Subject to Dual Regulation by OmpR

“…Gel slices containing the appropriate bands, visualized by Coomassie Blue staining, were excised with a clean scalpel. Prior to the LC-MS/MS analysis, the gel slices were subjected to in-gel trypsin digestion [ 33 ]. LC-MS/MS analysis of proteins was performed at the Mass Spectrometry Laboratory, IBB PAN, Warsaw.…”

“…It was found that OmpR is involved in protecting cells against adverse intracellular conditions experienced following macrophage phagocytosis [ 29 ]. OmpR participates in the expression of OmpC, OmpF and OmpX porins, and also important pathogenicity determinants like the Yop proteins, invasin, adhesin Ail and flagella [ 29 , 30 , 31 , 32 , 33 , 34 ]. Moreover, OmpR appears to modulate the adhesion and invasion abilities of Y .…”

The pathogenic Yersiniae – advances in the understanding of physiology and virulence

“…Flores-Kim and Darwin (2012) describe how these ECS responsive pathways are also important for the control of Yersinia virulence determinants; particularly those embedded in the bacterial envelope such as integral membrane-spanning T3SSs and the well-known surface adhesin invasin. The environmental control of invasin gene expression in Y. enterocolitica is further explored by Brzostkowska et al (2012). They demonstrate that OmpR, a response regulator of the EnvZ/OmpR two-component regulatory system, binds the inv promoter to directly negatively influence invasin expression.…”

The pathogenic Yersiniae—advances in the understanding of physiology and virulence

“…Flores-Kim and Darwin (2012) describe how these ECS responsive pathways are also important for the control of Yersinia virulence determinants; particularly those embedded in the bacterial envelope such as integral membrane-spanning T3SSs and the well-known surface adhesin invasin. The environmental control of invasin gene expression in Y. enterocolitica is further explored by Brzostkowska et al (2012). They demonstrate that OmpR, a response regulator of the EnvZ/OmpR two-component regulatory system, binds the inv promoter to directly negatively influence invasin expression.…”