On a Possible New Kind of Toxic Substance Produced by Bordetella Pertussis

Characterization of the Body Weight-Decreasing Toxicities in Mice by the Lymphocytosis-Promoting Factor and the Heat-Labile Toxin of B. Pertussis and Endotoxin

“…The supernatant of submerged culture of B. pertussis strain 3779B (Kurokawa, Iwasa and Ishida, 1965) in Cohen-Wheeler's liquid medium added with activated charcoal was subjected stepwise to (1) ammonium sulphate precipitation, (2) elution in phosphate buffer, (3) ultracentrif ugation at 100,000 g, and (4) gel-filtration through Sephadex G-100 (Iwasa et al, 1968). Two preparations obtained at the step (2), Prl-14 and Prl-16, and two preparations at the step (4), F39 and 78E, were used. This method of fractionation originally aimed at the purification of the entity re-BY B. PERTUSSIS AND ENDOTOXIN 117 sponsible for the late appearing toxicity in mice (Kurokawa et al, 1965).…”

Attempts at Analysis of Toxicity of Pertussis Vaccine I. Body Weight-Decreasing Toxicity in Mice

“…No significant deviation from this model had been (Pittman & Cox, 1965) or the late appearing toxicity (Kurokawa et al, 1965). This was shown in mice inoculated with certain preparations of pertussis vaccine and some properties of the * The difference between the body weight on the day of vaccine inoculation and that on the ith day after the inoculation was symbolized as BWD1 (i=1, 2•c•c) (Kurokawa et al, 1962 a;Ishida, 1968).…”

“…The substance suggested as the entity responsible for the second valley (Kurokawa et al, 1965) and designated as LPF (Iwasa et al, 1966(Iwasa et al, , 1968 may be ranked as the 4th one.…”

Lymphocytosis-Promoting Factor Produced by Bordetella Pertussis Partial Purification and Characterization

“…Attempts have been made to separate the entity responsible for the lymphocytosis from pertussis cells, but none succeeded in obtaining the material practically free of other known biological activities. Kurokawa, Iwasa and Ishida (1965) reported a toxic factor produced by pertussis bacilli showing such late-appearing toxicities (LAT) as late body-weight loss and late death of mice. During the course of purification of the toxic factor, it was noted that every fraction showing LAT for mice is accompanied by lymphocytosis-promoting activity (LPA).…”

“…pertussis strain, No. 3779B (Kurokawa, Iwasa and Ishida, 1965) was cultured in a modified Cohen-Wheeler's medium containing 0 .04% activated charcoal and 0.0001 % DL-tryptophan at 37 C for 15 to 20 hr on a shaker. The culture was added with 0.01% merthiolate and kept for a few days at room temperature to be sterilized .…”