On-Chip Immunoassay for Determination of Urinary Albumin

Laiwattanapaisal, Wanida; Songjaroen, Temsiri; Maturos, Thitima; Lomas, Tanom; Sappat, Assawapong; Tuantranont, Adisorn

doi:10.3390/s91210066

Cited by 28 publications

(17 citation statements)

References 33 publications

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“…Although a similar microchip-based system has been implemented in our group 17,18 , the fluorescence experiment to examine the mixing capability had yet to be investigated. To verify the results acquired from the fluorescence image acquisitions, it had to be assured that the mixing results would be identical to those of the computational simulation.…”

Section: Simulation and Evaluation Of Mixing Efficiencymentioning

confidence: 99%

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

et al. 2011

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The ability for low reagent consumption and minimum waste production in a miniaturised system has generated great interest in the green chemistry field. Herein, a microfluidic system for calcium assays using the arsenazo III method has been developed. The reaction between arsenazo III and calcium to form a blue-purple coloured complex is measured by an embedded miniature fibre optic spectrometer through absorbance increments at 650 nm. A linear range was obtained from 0.2 to 3 mg dL -1 with a detection limit of 0.138 mg dL -1 (S/N=3). The method exhibited good reproducibility based on low and high calcium tests with control serums, the within-run coefficient of variation (CVs) (4.10% and 3.91%), and the run-to-run CV (4.6%) were obtained. The carry-over effect of the method was also 1.98%, which is acceptable for the current system. When compared to a conventional spectrophotometric method, this portable, microfluidic method correlated highly when evaluating serum samples (r 2 =0.985; n=15). This similarity suggests that our proposed system could be used for determining the amount of calcium in serum samples.

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Section: Simulation and Evaluation Of Mixing Efficiencymentioning

confidence: 99%

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

et al. 2011

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“…The short optical path length limits the applications of this method because of micron-or nano-sized channel. Laiwattanapaisal et al (2009) performed an immunoassay on a portable microfluidic device to measure urinary albumin by monitoring the absorbance changes. For increasing the Fig.…”

Section: Absorbance Detectionmentioning

confidence: 99%

Microfluidic whole-blood immunoassays

Jiang

Weng

2010

Microfluid Nanofluid

100

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Immunoassay is one of the most widely used biomedical diagnostic methods due to its sensitivity and specificity. Microfluidic lab-on-a-chip technology has the advantages of portability, integration, and automation. The combination of these two technologies leads to a pathway for point-of-care diagnostics using the unprocessed samples such as the whole blood. This article reviews the recent advancement and the major development in the microfluidic-based whole-blood immunoassays. After a survey of the recent studies on microfluidic whole-blood immunoassays, an in-depth review about the detection methods that can be miniaturized and integrated in the immunoassay chips is provided. Point-of-care diagnostics applications require developing a fully integrated, disposable, low-cost, and handheld microfluidic device for the whole-blood immunoassay. In this regard, some comments and suggestions for future research are given.

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“…has developed an on-chip immunoassay for detecting urinary albumin with performance comparable to conventional spectrophotometric method. 65 However, the immunoassay-based protein detection often means tedious procedure and high reagent costs. In order to overcome these limitations, the Lee group developed non-immunological urinary albumin sensors, either based on electrochemical detection 66 or specific dye binding 67 .…”

Section: Microfluidic Detection Of Nutritional Biomarkersmentioning

confidence: 99%

Microfluidic opportunities in the field of nutrition

Kiehne

Sinoway

et al. 2013

Lab Chip

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Nutrition has always been closely related to human health, which is a constant motivational force driving research in a variety of disciplines. Over the years, the rapidly emerging field of microfluidics has been pushing forward the healthcare industry with the development of microfluidic-based, point-of-care (POC) diagnostic devices. Though a great deal of work has been done in developing microfluidic platforms for disease diagnoses, potential microfluidic applications in the field of nutrition remain largely unexplored. In this Focus article, we would like to investigate the potential chances for microfluidics in the field of nutrition. We will first highlight some of the recent advances in microfluidic blood analysis systems that have the capacity to detect biomarkers of nutrition. Then we will examine existing examples of microfluidic devices for the detection of specific biomarkers of nutrition or nutrient content in food. Finally, we will discuss the challenges in this field and provide some insight into the future of applied microfluidics in nutrition.

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On-Chip Immunoassay for Determination of Urinary Albumin

Cited by 28 publications

References 33 publications

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

Microfluidic whole-blood immunoassays

Microfluidic opportunities in the field of nutrition

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