On-chip microband array electrochemical detector for use in capillary electrophoresis

Slater, Jonathan M.; Watt, Esther J.

doi:10.1039/an9941902303

Cited by 20 publications

(15 citation statements)

References 21 publications

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“…5,6,8,[22][23][24][25] In this paper, we present a simple chip CE system that employs a novel injection protocol and an electrochemical detector. The injection procedure is fast and reproducible and does not require precise voltage control of electrolyte reservoirs.…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

“…A good signalto-noise ratio is obtained by the unique characteristics of hydrodynamic microelectrodes employed in wall-jet detector configuration.21 Since this approach circumvents the need for a special decoupler device, therefore making the chip easier to manufacture, it has been favored by many groups for CE-EC in the chip format and applied to a variety of analytes. 5,6,8,[22][23][24][25] In this paper, we present a simple chip CE system that employs a novel injection protocol and an electrochemical detector. The injection procedure is fast and reproducible and does not require precise voltage control of electrolyte reservoirs.…”

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confidence: 99%

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A Chip-Based Electrophoresis System with Electrochemical Detection and Hydrodynamic Injection

2002

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A simple capillary electrophoresis system in the planar format that uses a new, hydrodynamic injection principle is described. The system was realized with poly(dimethylsiloxane)-glass chips and microdisk electrodes for amperometric detection. Using a double-tee injector, no precise voltage control of the electrolyte reservoirs was needed, thus making the microchip CE system more user-friendly. The analytical characteristics of chip-based CE-EC were evaluated using ascorbic acid as the model analyte. The reproducibility of migration time and signal height was expressed by relative standard deviations of 1.2% and 5.1%, respectively (n = 5). The limit of detection for ascorbic acid was ~5 μM at a signal-to-noise ratio of 3. Practical application concerning the determination of physiologically relevant compounds such as noradrena-line and L-dopa are discussed.In recent years, there has been a significant interest in miniaturized analytical systems. The downscale in electrophoresis results in a decrease in analysis time and a potential increase in efficiency while the instrument becomes more compact due to integration of essential parts onto a single substrate.1,2 Another attractive feature is the possibility of parallel analysis using multiple separation systems residing on one chip. Capillary electrophoresis (CE) in the planar format has been applied to a variety of analytes, e.g., DNA,3,4 neurotransmitters,5,6 explosives,7 and the bioassay of clinically relevant compounds.8 Fabrication of microfluidic devices in poly(dimethylsiloxane) (PDMS) by soft lithography provides a fast, inexpensive route to devices that can handle aqueous solutions.9 These soft lithographic methods are based on rapid prototyping and replica molding and are easily accessible to chemists and biologists working under benchtop conditions. Crucial in chip-based CE is the reproducible introduction of well-defined sample zones in the separation channel and the detection of narrow bands. While detection schemes, such as electrochemical detection (EC) or laser-induced fluorescence (LIF), can be adapted from conventional CE, sample introduction requires a completely new approach.In microchip CE, injection is usually performed by electro-kinetic methods. The introduction of a well-defined sample plug is achieved through the channel network, i.e., a sample-guiding channel that intersects the separation channel. The first CE chips employed a tee-injector design.10 Due to difficulties in the control of the sample plug, other injector layouts were developed. Improved control of the sample plug can be gained by using a cross11 or double-tee injector,12 which works well for the analysis of DNA using polymer sieving media.3,4 For injection, an electric field is applied across the sample reservoir and the sample waste reservoir. The resulting analyte stream is perpendicular to the separation channel. In the separation phase, an electric field is applied across the separation channel. The sample plug, residing in the channel intersection, is transpor...

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Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

mentioning

confidence: 99%

A Chip-Based Electrophoresis System with Electrochemical Detection and Hydrodynamic Injection

2002

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“…Slater and Watt [83] have described a microfabricated detector that incorporated a platinum microband array electrode and three larger electrodes on a silicon chip. A Na®on-based decoupler was implemented and the detector chip was connected with a conventional separation capillary.…”

Section: Electrochemical Detection For Chip and Channel Electrophoresismentioning

confidence: 99%

End-Column Electrochemical Detection for Capillary Electrophoresis

Matysik¹

2000

Electroanalysis

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Amperometric and voltammetric techniques are very attractive for detection in capillary electrophoresis (CE). The low limits of detection attainable with capillary electrophoresis‐electrochemical detection (CE‐ED) are a definite advantage over the widely used on‐column UV detection for CE. To fully exploit the potential of CE‐ED and to make it accessible to the non‐specialist, robust and user‐friendly detector devices have to be developed. This article reviews recent advances in the field of end‐column CE‐ED which can be operated without the implementation of an electrical‐field decoupler. Emphasis is given on the discussion of methodical aspects and on the design of decoupler‐free detector configurations that are promising to enhance the practicality of CE‐ED.

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“…Nowadays, microfluidics has been widely applied in wearable devices . Microfluidics is a multidisciplinary technology for precise manipulation of minute amounts of fluids in a confined micro space, which has been widely applied in various areas, including but not limited to organ on Chip, drug delivery, cell biology, and electrochemical detection . Microdroplets is another big category in microfluidics and offers a great number of opportunities in chemical and biological research, such as chemistrode and single cell/molecule assays …”

Section: Introductionmentioning

confidence: 99%

Application of Microfluidics in Wearable Devices

et al. 2019

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Wearable devices integrated with various electronic modules, biological sensors, and chemical sensors have drawn large public attention. Due to their inherent advantages of superior stretchability, elaborate microstructure, high integration of multiple functions, and low cost, microfluidics are an excellent candidate and have already been widely used in wearable devices. Well‐designed microfluidic devices can realize excellent multiple functions in wearable devices, including sample collecting, handling and storage, sample analysis, signal converting and amplification, mechanic sensing, and power supplying. Moreover, the microfluidic wearable devices with further integration of wireless modules have exhibited potential applications in healthcare monitoring, clinical assessment, and human and intelligent device interaction. This review focuses on the latest advances on multifunctional wearable devices based on microfluidics, primarily including general functions and designs of microfluidic wearable devices, and their specific applications in physiological signal monitoring, clinical diagnosis and therapeutics, and healthcare.

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On-chip microband array electrochemical detector for use in capillary electrophoresis

Cited by 20 publications

References 21 publications

A Chip-Based Electrophoresis System with Electrochemical Detection and Hydrodynamic Injection

A Chip-Based Electrophoresis System with Electrochemical Detection and Hydrodynamic Injection

End-Column Electrochemical Detection for Capillary Electrophoresis

Application of Microfluidics in Wearable Devices

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