On-chip signal amplification of magnetic bead-based immunoassay by aviating magnetic bead chains

Jalal, Uddin M.; Jin, Gyeong J.; Eom, Kyu Shik; Kim, Min Ho; Shim, Joon S.

doi:10.1016/j.bioelechem.2017.11.001

Cited by 24 publications

(9 citation statements)

References 27 publications

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“…Such amplification allows the detection of 1 ng/mL NGAL concentration in 5-fold diluted FBS, while this concentration could not be resolved by observing the fluorescent intensity of single beads. Common approaches for beads' accumulation involve the application of magnetic forces [29,30] or DEP forces [18]. The increase in signal that we found to be a consequence of the beads' accumulation is in line with what has been previously reported in literature [29].…”

Section: Immunoassay Performancesupporting

confidence: 92%

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

et al. 2020

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The simplicity of homogeneous immunoassays makes them suitable for diagnostics of acute conditions. Indeed, the absence of washing steps reduces the binding reaction duration and favors a rapid and compact device, a critical asset for patients experiencing life-threatening diseases. In order to maximize analytical performance, standard systems employed in clinical laboratories rely largely on the use of high surface-to-volume ratio suspended moieties, such as microbeads, which provide at the same time a fast and efficient collection of analytes from the sample and controlled aggregation of collected material for improved readout. Here, we introduce an integrated microfluidic system that can perform analyte detection on antibody-decorated beads and their accumulation in confined regions within 15 min. We employed the system to the concomitant analysis of clinical concentrations of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Cystatin C in serum, two acute kidney injury (AKI) biomarkers. To this end, high-aspect-ratio, three-dimensional electrodes were integrated within a microfluidic channel to impart a controlled trajectory to antibody-decorated microbeads through the application of dielectrophoretic (DEP) forces. Beads were efficiently retained against the fluid flow of reagents, granting an efficient on-chip analyte-to-bead binding. Electrokinetic forces specific to the beads’ size were generated in the same channel, leading differently decorated beads to different readout regions of the chip. Therefore, this microfluidic multianalyte immunoassay was demonstrated as a powerful tool for the rapid detection of acute life-threatening conditions.

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Section: Immunoassay Performancesupporting

confidence: 92%

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

et al. 2020

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“…Magnetic beads have been widely used to increase the binding affinity. 46,47 However, it is difficult to incorporate beads into the microfluidic channel as they easily settle down and get stuck in the channel. In vivo detection is also challenging as the beads emit strong auto-fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Bao

et al. 2020

Preprint

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A fully Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) system is developed for viral DNA detection. This powerful system is patterned with high-aspect ratio micropillars to enhance reporter probe binding. After surface modification and probe immobilization, CRISPR Cas12a/crRNA complex is injected into the fully enclosed system. With the presence of double-stranded DNA target, the CRISPR enzyme is activated and non-specifically cleaves the ssDNA reporters initially immobilized on the micropillars. This collateral cleavage releases fluorescence dyes into the assay, and the intensity is linearly proportional to the target DNA concentration ranging from 0.1 to 10 nM. Importantly, this system does not rely on traditional dye-quencher labeled probe thus eliminating the fluorescence background presented in the assay. Furthermore, our one-step detection protocol is performed at isothermal conditions (37°C) without using complicated and time-consuming off-chip probe hybridization and denaturation. This miniaturized and fully packed IMPACT chip demonstrates rapid, sensitive, and simple nucleic acid detection and is an ideal candidate for the next generation molecular diagnostic platform for point-of-care (POC) applications, responding to emerging and deadly pathogen outbreaks.

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“…2 Magnetic objects include not only intrinsically magnetic samples such as magnetotactic bacteria, 3 but also samples that are labelled with magnetic particles. 4 The use of magnetic particles specically conjugated to targets offer great selectivity allowing various applications for separation or detection, including immunomagnetic separation of cells, 5 proteins, 6 and nucleic acids, 7 the detection of pathogens. 7 Besides, with the aid of controllable magnetic forces by designing magnetic elds, cells can guide toward designed spatial arrangements, 8,9 which make it suitable for the organization of cells such as to align and migrate cells into a targeted pattern 10 and to pattern cells at specic points.…”

Section: Introductionmentioning

confidence: 99%

Microscale magnetic field modulation using rapidly patterned soft magnetic microstructures

et al. 2021

RSC Adv.

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On-chip signal amplification of magnetic bead-based immunoassay by aviating magnetic bead chains

Cited by 24 publications

References 27 publications

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Microscale magnetic field modulation using rapidly patterned soft magnetic microstructures

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