On-Chip Studies of Magnetic Stimulation Effect on Single Neural Cell Viability and Proliferation on Glass and Nanoporous Surfaces

Abstract

Transcranial magnetic stimulation (TMS) is a noninvasive neuromodulation technique, an FDA-approved treatment method for various neurological disorders such as depressive disorder, Parkinson's disease, post-traumatic stress disorder, and migraine. However, information concerning the molecular/cellular-level mechanisms of neurons under magnetic simulation (MS), particularly at the single neural cell level, is still lacking, resulting in very little knowledge of the effects of MS on neural cells. In this paper, the effects of MS on the behaviors of neural cell N27 at the single-cell level on coverslip glass substrate and anodic aluminum oxide (AAO) nanoporous substrate are reported for the first time. First, it has been found that the MS has a negligible cytotoxic effect on N27 cells. Second, MS decreases nuclear localization of paxillin, a focal adhesion protein that is known to enter the nucleus and modulate transcription. Third, the effect of MS on N27 cells can be clearly observed over 24 h, the duration of one cell cycle, after MS is applied to the cells. The size of cells under MS was found to be statistically smaller than that of cells without MS after one cell cycle. Furthermore, directly monitoring cell division process in the microholders on a chip revealed that the cells under MS generated statistically more daughter cells in one average cell cycle time than those without MS. All these results indicate that MS can affect the behavior of N27 cells, promoting their proliferation and regeneration.

Assessment of the corrosion resistance of self-ordered anodic aluminum oxide (AAO) obtained in tartaric-sulfuric acid (TSA)

“…These treatments contrast with TSA industrial anodizing, that requires only one anodization during less than 30 minutes, temperatures above room temperature and give rise to disordered porous anodic layers with 2-7 microns of thickness [42]. To date, ordered porous anodic layers have been applied to numerous fields as such as separation [43], chemical/biological sensing devices [44], cell adhesion [45], catalysis [46], energy storage [47] and drug delivery vehicles [48], being the most remarkable the employment as a template for the synthesis of nanostructures inside their pores [40,49].…”

Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

“…As model surface it was chosen to use glass cover slips as they are widely adopted to investigate cell behaviors on bio-inert surfaces. Glass coverslips are also transparent and can thus be used to observe both live and fixed cells using light or fluorescent microscopy (Islam et al, 2016;Van Dijk et al, 2017a;Che et al, 2018). In addition, we also investigated the effect of Hst1 on cell spreading on titanium SLA surface -a most commonly used surface for dental implants.…”

Topology Optimization of Passive Cell Traps

“…The microfluidic chip provides a platform for analyzing single cells by combining active or passive single-cell capture methods. Active single-cell capture methods, such as dielectric electric [ 2 , 3 , 4 , 5 ], optical [ 6 , 7 , 8 , 9 ], magnetic [ 10 , 11 , 12 , 13 , 14 ], or acoustic field [ 15 , 16 , 17 ], can provide precise cell manipulation and selective cell capture. However, these methods require external devices and need precise control.…”