On-column UV absorption detector for open tubular capillary zone electrophoresis

Near-infrared laser-induced fluorescence detection in capillary electrophoresis

“…This mixture was then directly injected into the CE column, which consisted of a run buffer of 20 mM borate, pH 9.2. The mass detection limits for the proteins stained with DTTCI were found to range from 1.3 to 3.7 amol, which is approximately three orders of magnitude lower than that reported for UV absorption detection [48,49]. However, it was found that the plate numbers for the separation were low, which was attributed to dissociation of the dye/protein complex during the separation, resulting in a change in the mobility of the complex during migration.…”

Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography

“…Since this cell can be the most significant contributor to extra-column broadening [15], alternate detection modes have also been surveyed. On-column detection has been employed to eliminate extra tubing requirements, but the low path length tends to hurt sensitivity [56,[79][80][81][82][83] and similar measurements using fluorescence require derivatization for many analytes [84][85][86][87]. An alternative option with a low limit of detection and reduced volume is electrochemical detection with a microfiber electrode [49,[88][89][90].…”

Separation of nonsteroidal anti-inflammatory drugs by capillary electrophoresis using nonaqueous electrolytes

“…The use of nonaqueous electrolyte has gained attention in various publications because of the better solubility of hydrophobic analytes [7±23]. Already in 1984, a separation in an acetonitrile buffer containing hydrochloric acid and tetraethylammonium perchlorate was described by Walbroehl, for the analysis of quinoline derivatives [7]. Benson et al used nonaqueous electrolytes containing ammonium acetate and acetic acid in methanol for the CE separation of hydrophobic metabolites of the antitumor drug pyrazoloacridine [8] and of the H 2 antagonist mifentidine [9,10].…”