On-demand, parallel droplet merging method with non-contact droplet pairing in droplet-based microfluidics

Lee, Sang‐Hyun; Kim, Hojin; Won, Dong‐Joon; Lee, Jaehyung; Kim, Joonwon

doi:10.1007/s10404-015-1676-z

Cited by 126 publications

(34 citation statements)

References 33 publications

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“…The mean width was 232 µm (SD = 25 µm), 427 µm (SD = 16 µm), and 652 µm (SD = 41 µm) for the nominal width of 200, 400, and 600 µm respectively. The deviation in width we obtained is smaller compared to 67.8 µm reported by a characterization test performed on parts printed by an FDM 3D printer . Optical image depicting cross‐section of microchannel after removal of mold showed morphology of the removed mold (Figure c).…”

Section: Resultscontrasting

confidence: 52%

“…Recent advances in additive manufacturing technologies have paved a way to fabricate microfluidic devices with 3D design of channels . Examples of additive manufacturing include selective laser sintering, stereolithography (SLA), laminated object manufacturing, inkjet printing of photopolymer, and fused deposition modeling . Generally, these additive manufacturing technologies require a 3D computer‐aided design (CAD) model of interest transformed into a computer code describing the model sliced in layers.…”

Section: Introductionmentioning

confidence: 99%

“…The codes are used to print the 3D object layer‐by‐layer in vertical dimension. At present, the entire microfluidic channel has been fabricated by SLA, polymer jetting, and FDM . 3D printing has exhibited prospects to be methods for fabrication of microchannels alternative to softlithography.…”

Section: Introductionmentioning

confidence: 99%

“…3D printing has exhibited prospects to be methods for fabrication of microchannels alternative to softlithography. Potential drawbacks of 3D printing are surface roughness, optical clarity, biocompatibility, and selection of materials available for printing …”

Section: Introductionmentioning

confidence: 99%

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Fabrication of 3D Microfluidic Channels and In‐Channel Features Using 3D Printed, Water‐Soluble Sacrificial Mold

Goh

Hashimoto

2018

Macro Materials & Eng

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Recent advent of additive manufacturing potentiates the fabrication of microchannels, albeit with limitations in resolution of printed structures, freedom of geometry, and choice of printable materials. Herein, a method is developed by sacrificial molding to fabricate microchannels in various polymer matrices and geometries. This method allows for rapid fabrication of 3D microchannels and channels harboring intricate in‐channel features. The method uses commercially available fused deposition modeling 3D printer and filament made of polyvinyl alcohol (PVA). Mechanically stable molds are fabricated for 3D microchannels that can be completely removed in water. Importantly, the PVA mold is stable and resilient in hydrogels despite being hygroscopic. Perfusion channels are fabricated in biocompatible substrates such as gelatin and poly(ethylene glycol) diacrylate. Fabrication of the network of 3D multilayer microchannels is demonstrated by preassembling sacrificial molds from modular pieces of molds. Intricate staggered‐herringbones grooves (SHGs) are also fabricated within microchannels to produce micromixers. The versatility and resilience of the method developed here is advantageous for biological and chemical applications that require 3D configurations of microchannels in various matrices, which would not be compatible with fabrication by direct 3D printing and softlithography.

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Section: Resultscontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of 3D Microfluidic Channels and In‐Channel Features Using 3D Printed, Water‐Soluble Sacrificial Mold

Goh

Hashimoto

2018

Macro Materials & Eng

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“…The mandrels were designed by the means of computer aided design (CAD) and then 3D printed with PolyJet (Stratasys, Eden Prairie, MN, USA) [53]. To systematically study the effect of mandrel rotation speeds on the average fiber dimensions, a cylindrical mandrel with a diameter of 30 mm was adopted in this work.…”

Section: Methodsmentioning

confidence: 99%

Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method

Tan

Yeong

et al. 2016

Materials

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Polymeric fibrous scaffolds for guiding cell growth are designed to be potentially used for the tissue engineering (TE) of tubular organs including esophagi, blood vessels, tracheas, etc. Tubular scaffolds were fabricated via melt-drawing of highly elastic poly(l-lactide-co-ε-caprolactone) (PLC) fibers layer-by-layer on a cylindrical mandrel. The diameter and length of the scaffolds are customizable via 3D printing of the mandrel. Thickness of the scaffolds was varied by changing the number of layers of the melt-drawing process. The morphology and tensile properties of the PLC fibers were investigated. The fibers were highly aligned with a uniform diameter. Their diameters and tensile properties were tunable by varying the melt-drawing speeds. These tailorable topographies and tensile properties show that the additive-based scaffold fabrication technique is customizable at the micro- and macro-scale for different tubular tissues. The merits of these scaffolds in TE were further shown by the finding that myoblast and fibroblast cells seeded onto the scaffolds in vitro showed appropriate cell proliferation and distribution. Human mesenchymal stem cells (hMSCs) differentiated to smooth muscle lineage on the microfibrous scaffolds in the absence of soluble induction factors, showing cellular shape modulation and scaffold elasticity may encourage the myogenic differentiation of stem cells.

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Patient‐Specific Organoid and Organ‐on‐a‐Chip: 3D Cell‐Culture Meets 3D Printing and Numerical Simulation

et al. 2021

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The last few decades have witnessed diversified in vitro models to recapitulate the architecture and function of living organs or tissues and contribute immensely to advances in life science. Two novel 3D cell culture models: 1) Organoid, promoted mainly by the developments of stem cell biology and 2) Organ‐on‐a‐chip, enhanced primarily due to microfluidic technology, have emerged as two promising approaches to advance the understanding of basic biological principles and clinical treatments. This review describes the comparable distinct differences between these two models and provides more insights into their complementarity and integration to recognize their merits and limitations for applicable fields. The convergence of the two approaches to produce multi‐organoid‐on‐a‐chip or human organoid‐on‐a‐chip is emerging as a new approach for building 3D models with higher physiological relevance. Furthermore, rapid advancements in 3D printing and numerical simulations, which facilitate the design, manufacture, and results‐translation of 3D cell culture models, can also serve as novel tools to promote the development and propagation of organoid and organ‐on‐a‐chip systems. Current technological challenges and limitations, as well as expert recommendations and future solutions to address the promising combinations by incorporating organoids, organ‐on‐a‐chip, 3D printing, and numerical simulation, are also summarized.

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On-demand, parallel droplet merging method with non-contact droplet pairing in droplet-based microfluidics

Cited by 126 publications

References 33 publications

Fabrication of 3D Microfluidic Channels and In‐Channel Features Using 3D Printed, Water‐Soluble Sacrificial Mold

Fabrication of 3D Microfluidic Channels and In‐Channel Features Using 3D Printed, Water‐Soluble Sacrificial Mold

Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method

Patient‐Specific Organoid and Organ‐on‐a‐Chip: 3D Cell‐Culture Meets 3D Printing and Numerical Simulation

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